Issue 2

A gross anatomical and histological study of pancreas in adult Kestrel (Falco tinnunculus)

Ammar Ghanim Al-Haaik

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 175-180
DOI: 10.33899/ijvs.2019.162960

Fifteen Kestrel were used in this study regardless to their sex. The results study showed that the pancreas of adult Kestrel (Falco tinnunculus) lies under the right side of the peritoneal cavity, and situated between the descending part and ascending part of the duodenum and has pale pinkish to white pinkish colored and consisted of 3 lobes dorsal (lobus pancreatic dorsalis), middle (middle pancreatic lobus) and ventral (lobus pancreatis ventralis) with well-developed interlobar connections made it difficult to distinguish between the 3 lobes. Histologically the pancreas of adult Kestrel (Falco tinnunculus) was covered by very thin layer of connective tissue with mesothelial cells, Connective tissue septa extended from capsule into parenchyma of pancreas dividing it in to many lobules. These septa were very thin and some of them carried blood vessels distributed all over the pancreas. The exocrine portion was constituted from serous acini with pyramidal shaped cells which had dark rounded nucleus in the middle part of the cell. Acidophilic granules found in the apices of cells. Centro acinar cells found as one or two nuclei in the center of the pancreatic acini. The endocrine portion (islets of Langerhans) was formed from clusters of endocrine cells in shape of oval or rounded pale structures with different sizes. The small one has mean diameter of 40.02±0.9 µm while the large one has diameter of 126.3±3.8 µm. Few endocrine cells were seen distributed as single cells among the pancreatic acini.

Prevalence of nematodes infestation in Clarias gariepinus from El-Burullus Lake and Lake Nasser, Egypt

Sh.S. Sorour; A.H. Hamouda

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 181-188
DOI: 10.33899/ijvs.2019.162963

A total number of 400 Clarias gaiepinus were collected alive from several and various locations at El-Burullus Lake and Lake Nasser, to investigate the prevailing nematodes that infect this fish species from the two locations. Nearly all the examined fish were positive for one or more nematodes; four spp. of two families were identified from Clarias gaiepinus at El-Burullus Lake: Procamallanus laeviconchus, Paracamallanus cyathopharynx, Neocamallanus spp. (recovered from the gall bladder for the first time) and Terranova spp. larvae (recorded for the first time in Kafr El-Sheikh Governorate representing new locality record), one nematode from Clarias gaiepinus at Lake Nasser: 3rd stage  larvae (L3) of Contraceacum spp. (has zoonotic importance). The prevalence of Procamallanus laeviconchus, Paracamallanus cyathopharynx, Neocamallanus spp. and Terranova spp. larva were 37.5%, 44%, 0.5% and 10% respectively, meanwhile the prevalence of L3 larvae of Contraceacum spp. were 100%. This study was planned to compare between the nematodes infecting Clarias gaiepinus from the two lakes evaluating clinical signs, postmortem examinations, parasitological examinations, seasonal prevalence and histopathological investigations of infected fish with different nematodes infestation. Clarias  gaiepinus must be eviscerated as soon as possible after catching to prevent 3rd stage larvae of Contraceacum spp. in the abdominal cavity from liberating from their sheaths and attacking the fish musculature (the edible part in the fish) as well as,prevent Terranova spp. larvae in the gills from attacking another parts of the fish and thus prevent the transmission of the nematodes of zoonotic importance to the consumers.

Study of histopathological and biochemical effect of Punica granatum L. extract on streptozotocin -induced diabetes in rabbits

E.R. Sarhat; S. A. Wadi; Th. R. Sarhat; N.A. Jasim

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 189-194
DOI: 10.33899/ijvs.2019.125523.1045

This study was undertaken to determine the antidiabetic effects of oral administration of Punica granatum L. extract on serum and tissues of streptozotocin induced diabetic rabbits at 100 mg/kg. The present study was carried out at the Faculty of veterinary Medicine, Tikrit University, from February to August 2017 for 10 weeks. For this purpose, 30 rabbits were randomly separated into three groups, each containing 10 animals: Group 1, healthy control rabbits; Group 2, diabetic rabbits received streptozotocin (STZ, 65 mg/kg); Group 3, diabetic rabbits treated with PS extract (the 100 mg PS+1 ml DW) for 21 days. At the end of experiment, blood samples were taken for measuring serum biochemical parameters. For histopathological evaluation, sections of kidneys were fixed in 10% buffered formalin and 5micron thick sections with H&E stain were prepared using routine histopathological techniques. The treatment revealed that PSE extract significant decreased serum glucose throm-bospondin-1, nitric oxide, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase alkaline phosphatase, and C-reactive protein in diabetic treated rabbits as compared to diabetic rabbits. Histopathology of kidney showed lesions similar to human glomeruloscleroses, glomerular membrane thickening, arteriolar hyalinization and tubular necrosis. From the above one can conclude that PSE extract possess nephroprotective effect in experimentally induced diabetic rabbits.

Isolation and identification of Salmonella typhimurium bacteria with detection of type-1 fimbriae coding gene by polymerase chain reaction (PCR) technique

Zeena Foaad Saleh; B.M. Al-Muhana; Kh. Hamdan; M.S. Jawad; S.F. Klaif

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 195-199
DOI: 10.33899/ijvs.2019.162961

Two hundred faeces sample were collected from cattle with different age and sex in Al- Diwaniyah Province. The study was conducted in the period between November 2016 and November 2017. Salmonella typhimurium bacteria identified by routine methods such as culturing on selective media, biochemical test and agglutination test using monovalent and multivalent antisera. PCR was can detection type-1 fimbriae gene coding for fimC of Salmonella typhimurium. Results showed that Salmonella isolates were 14.5% in the bovine fecal samples. Also, the serotyping of isolates by using monovalent and polyvalent antisera revealed that all Salmonella isolates in cows were S. typhimurium. The PCR technique was used for detection of type-1 fimbriae coding gene by specific primer for fimC gene. All S. typhimurium isolates in cows appeared to be contained this gene show one distinct band MW.289 bp when electrophoresed on agarose gel. The results of this score indicated that the PCR technique potentate a loud specify in the disclosing of S. typhimurium especially the serotype that encoded to fimC gene type-1 fimbriae isolated from cows in comparison to other routine diagnostic tests.

Mixed thymic tumor in a rooster: gross and histological features

Saevan Saad Al-Mahmood; Ahmed Saad AL-Gadhi; Radhwan R. Al-Ajeli; layth Alkattan

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 201-204
DOI: 10.33899/ijvs.2019.125536.1058

A 2-year-old rooster local breed suffered from anorexia, emaciation with abnormal large mass in lateral side in cervical region was attended to Veterinary Teaching Hospital belong to College of Veterinary Medicine, Mosul University. After physical examination a movable and friable mass were recognized in cervical region. The case sends for surgical intervention. Gross examination of removed mass showed a friable, pale, will capsulated with hemorrhagic areas. Microscopically, the mass included three types of tumors. Squamous cell thymic carcinoma characterized by presence of keratin pearl. Lymphoma that characterized by atypical polymorphic lymphocytes, vesicular nucleus, and pyknotic nucleoli, while cytoplasm appears eosinophilic to granular with ill- define borders. Lipoma which appear as irregular shape of adipocytes that lack their nucleus, with scattered infiltration of pyknotic lymphocytes. This is the first pathological report of mixed tumor of thymus gland in rooster that composed from squamous cell thymic carcinoma, lymphoma and lipoma.

Early prediction of some kid body dimensions from maternal traits

Abdullah Hameed Salim; Ahmed Abdulridha Mnati; Yassen Taha Abdul-Rahman

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 205-207
DOI: 10.33899/ijvs.2019.125537.1059

This study was conducted in the Iraqi Ministry of Agriculture, Ruminant Research Station, west of Baghdad. It included 447 goats born of both sexes belonging to local, Shami and their crosses. The body dimensions of kids at birth and the dam's weight and age at birth, as well as the dependence of weaning weight on the birth weight of the kids by the use of simple linear regression equations. The results presented that coefficient of regression of most studied dimensions on the traits of maternal weight at birth and weight of the kid at birth has significant effect, except the regression of the body length at birth on both the maternal weight at birth and the kids weight at birth, which was not significant, The results showed the possibility of dependence on the studied maternal characteristics and kids birth weight to predict the dimensions of the goat kids body at birth.

Molecular identification of allelic genotypes of pyrethroid-insecticide resistance in housefly, Iraq

Monyer Abdulameir Abd Alfatlawi; Mansour Jadaan Ali; Hassan Hachim Naser

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 209-212
DOI: 10.33899/ijvs.2019.125538.1060

According to global-wide presence of insecticides resistance to pyrethroids, the current study identified the purpose to detect the allelic genotypes regarding this issue in house flies in Iraq. From the governorate of Al-Qadisiyah, Iraq, 60 morphologically and molecularly recognized house flies were caught from 6 different regions. Using a technique called polymerase chain reaction (PCR) amplification of specific allele (PASA), PCR was employed to reveal the presence of allele-genetic variations in the para-type sodium channel (para) gene to recognize knockdown resistance (kdr) mutation from the homozygous-wild type of complete susceptibility (sus/sus) to the mutated-homozygous type of complete resistance (kdr/kdr) or to the mutated-heterozygous type (kdr/sus). Here, these genotypes were targeted using specific primers to identify these genetic variations. The results have declared the presence of the sus/sus at 100%-frequency rate in all flies, and none of the other genotypes were detected (0%) in all flies. This valued piece of result indicates the reality of resistance persistence due to lack of insecticide-spraying programs in the governorate. This study provides high-quality information about the current status of insecticide resistance in house flies in Iraq about supporting the fact of genetic-base development of such resistance via frequent use of insecticides.

Effect of arginine on physiological and reproductive performance of Awassi ewes

Thair Mohammed; Ahmed Al-Ani; Alaa Munther

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 213-220
DOI: 10.33899/ijvs.2019.162878

The objective of the current study was to determine the effect of injected amino acid arginine on reproductive performance in ewes and on the levels of some hormones. Twenty-one non pregnant Awassi Turkish ewes were used. These ewes were divided into three equal groups. The first group were not treated which serve as control group (T1), and injected with 5-10 ml of normal saline in intramuscular. Ewes of second group (T2) were treated by injected with a 155µmol/kg arginine I.V. The third group (T3) was treated by injected with 155µmol/kg arginine intramuscular. All treated ewes were inseminated by rams for 7 days. Blood samples were taken from jugular vein on the 1st and 2nd days of arginine injection and then insert CIDR and on the 3rd, 5th, 7th, 10th,13th, and 16th days of this treatment to measure the change in the levels of some hormones during treatment. The results showed arithmetic difference in the time of the estrus where the treatment of intramuscular arginine injection came first and it was followed by intravenous arginine injection and then the control. Arginine treated groups significantly the control group in the estrus appearance rate. The estrus appearance estrus rate in intravenous injection group, intramuscular injection group and control group were 71.43, 57.14, and 42.85% respectively, also there is significant increase in fertility rates were 71.43, 57.14 and 42.85% respectively. There was a significant increase in pregnancy rates were 71.43, 57.14 and 28.85% respectively. However, birth rates were 71.43, 57.14 and 28.57% respectively. Intravenous treatment showed the lowest than other treatments. The results of current research revealed no significant difference between groups in the level of progesterone hormone, furthermore there is no significant change in the hormone during all periods of the experiment except the 7th period in IV arginine treated group. It was also observed a significant decrease in the estrogen concentration level in arginine intravenous (14.62 ±1.02) and intramuscular (15.00 ±1.98) injected groups in compared with control group (16.82 ±1.25) respectively during the first and 7th periods of injection after the insert of CIDR as compared with the other periods. There were no significant differences in the level of cortisol between the treated groups and the control group, and also between all periods of the experiment. It was concluded from this study that injection of amino acid arginine improved the reproductive performance of Awassi Turkish ewes, taking into consideration the superiority of intravenous injector as compared with intramuscular injection which gave higher rates of estrus, fertilization, fertility, pregnancy and birth and gave the lowest proportion of barrenness as compared with control group. However, arginine had no significant effects on the level of estrogen, progesterone and cortisol.

Effect of probiotic (Poultrystar®) and heat stress on some blood parameters in common carp (Cyprinus carpio L.)

Rawnaq H. Ismail; Alaa Alhamdani

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 221-225
DOI: 10.33899/ijvs.2019.125539.1061

This study was conducted to determine the effect of the heat stress on some blood parameters and role of probiotics (Poultrystar®) to reduce these effects, 120 common carp (Cyprinus carpio) with mean weight 150±10g were obtained from collage of Agriculture and forestry /University of Mosul. Fish were acclimated for at least 7 day in the laboratory and fed daily, the water temperature was maintained at 25 ±2°C. Three experiments have been done, each of 40 fish which have been divided randomly into four groups 10 for each. First experiment the four groups exposed to different water temperature (control 25, 30, 32, 34 °C), second experiment the four groups treated with (Poultrystar®) in a dose 1 g/ kg body weight by using stomach tube with exposed to heat stress (control 25, 30, 32 and 34 °C). In the third experiment the four groups have been treated with (Poultrystar®) in the same dose of the previous experiment for 7 days then exposed to heat stress. The results show there were significantly increase in hemoglobin concentration in three experiments, and also the Packed cell volume was significantly increase in three experiments, but the stress index was significantly decrease in three experiments, and the probiotics (Poultrystar®) improved the blood picture especially in the second and third experiment because no probiotic used in the first experiment.

Variations in the viability and macromolecules concentration of E. granulosus protoscolices isolated from ruminants consequence treatment with Nigella sativa seed's oil (In vitro study)

Maimonah Q. Yahya

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 227-234
DOI: 10.33899/ijvs.2019.162880

The aim of the present study was to investigate the scolicidal activity of Nigella sativa seed's oil concerning the viability in vitro and biomolecules content (carbohydrates, proteins and nucleic acids) ofEchinococcus granulosus protoscolices. Protoscolices were aseptically aspiratedfromcysts of livers and lungs (naturally infected) which eradicated from goats and sheep that had been slaughtered at Mosul local abattoir / Nineveh / Iraq from September 2017 to May 2018. Various concentrations of N. sativa seed's oil (20 to 60 µl/ml) were applied on special time interval (5-40min). Viability of protoscolices was checked using vital stain (0.1% aqueous eosin). Concentration of carbohydrates, proteins and nucleic acids (DNA and RNA) were estimated after treating the protoscolices with LC50 of the seed's oil (40 µl/ml) comparing with untreated group. It is revealed that mortality rate of protoscolices, those were treated for 10 minutes with 40 µl/ml and 60 µl/ml of Nigella sativa oil, were approximately 50% and 100% respectively. Viability % of protoscolices treated with 20% hypertonic saline solution for 5 minutes was 43%, whereas, 16% of protoscolices were viable when treated with 60 µl/ml of seed's oilat the same time. The experiments detect concentration and time-dependent scolicidal effect of N. sativa seed's oilon the E. granulosus protoscolices. Mean concentrations of carbohydrates, proteins and nucleic acids (DNA and RNA) were significantly higher at P≤ 0.05 in control group (62.6µg/ml, 31.0 mg/dl and (23.4 and 82.9 µg/ml, respectively) than that found in LC50 treated protoscolices (58.3 µg/ml, 15.3 mg/dl and (18.19 and 64.48 µg/ml, respectively).The study showed that oil extract of N. sativa seedshas a significant (P≤ 0.05) clear impact in reducing viability of E. granulosus protoscolices, as well as, mean concentrations of its biomolecules which may open away for further experiments about scolicidal validity of N. sativa seeds oil in vivo.

Comparative pharmacokinetic study of theaflavin in healthy and experimentally induced liver damage rabbits

Sarhan Rashid

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 235-242
DOI: 10.33899/ijvs.2019.162962

This current work aimed to study the pharmacokinetics of theaflavin in healthy and hepatotoxic rabbits for comparison. Aspartate aminotransferase (AST), alkaline phosphatase (ALP) and alanine aminotransferase (ALT) were significantly raised (P<0.05) after administration of 0.2 mg/kg body weight (BW) Carbone tetrachloride (CCL4) subcutaneously. Pharmacokinetic parameters calculated following administration of theaflavin intravenously and orally at 30 mg/kg and 500 mg/kg respectively to both healthy animals and those with damaged liver. Theaflavin concentration in blood measured by HLPC at various time intervals. Pharmacokinetic results showed that theaflavin concentration when given orally reached its maximum concentration after 5 hours in healthy rabbits. While in hepatotoxic group, theaflavin concentration achieved the highest level in blood after three hours. Theaflavin bioavailability in hepatotoxic animals was significantly high and almost double its bioavailability in healthy animals. Results revealed that the area under curve (AUC) value in rabbits with damaged liver was significantly greater than in healthy group (P<0.05). t ½ of theaflavin after intravenous administration was 6.3±0.82 hour in damaged liver group which is significantly higher than that in healthy group (P<0.05). Theaflavin mean concentration in hepatotoxic group required more than 3 hours to decline to 352±19.4 ng/ml when compared to its concentration in healthy group which is required only 45 minutes to decrease to 310± 9.5 ng/ml. In conclusion liver has critical impact on the pharmacokinetics of theaflavin especially bioavailability and biotransformation and this research recorded reasonably large differences between healthy and liver damaged groups regarding theaflavin pharmacokinetic parameters which may result in negative influences on its biological efficacy when used in the treatment of various diseases.

Experimental detection of antioxidant and atherogenic effects of grapes seeds extracts in rabbits

Shihaab Al-Bajari; Moataz Al- Akash; Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 243-249
DOI: 10.33899/ijvs.2019.162881

The aim of this study was to determine the ability of Grapes seeds (Vitis vinifera) aqueous, proteinous, non- proteinous and alcoholic extracts as antioxidant and antiatherogenic in rabbits after exposure to 0.5% H2O2 in drinking water for 30 days. The results illustrated capability of 0.5% H2O2 to initiate oxidative stress via significant decrease in Glutathione (GSH), glutathione peroxdiase (GPx), Catalase (CAT) and superoxide dismutase (SOD) level in heart and liver concomitant with significant increase in malondialhyde (MDA) in addition to decrease in high density lipoprotein- cholesterol (HDL-c) and increase low density lipoprotein- cholesterol (LDL-c) in serum of treated only with H2O2 rabbits. While rabbits treated with H2O2 associated with Grapes seeds (aqueous, proteinous, non- proteinous and alcohol) extracts for 15 days showed a significant increase of Glutathione (GSH), glutathione peroxdiase (GPx), superoxide dismutase (SOD) concentration in the heart and liver tissue as well as increase HDL-c, decrease in LDL-c and VLDL-c. While, the histolopathological examination of heart and liver tissue demonstrated that H2O2 induces, degeneration, necrosis and few inflammation foci in the liver and myocardial muscle. While the administration of (Vitis vinifera) aqueous, proteinous, non-proteinous and alcoholic extracts cause improvement in the histological picture of the liver and the myocardial muscle. It was concluded that the (Grapes extracts) aqueous, proteinous, non-proteinous and especially the alcoholic extracts can reduce the damage caused by H2O2 and this will pave the way to investigate the protective effects of the natural substances in the diseases caused by oxidative stress.

Microscopic identification, molecular and phylogenetic analysis of Babesia species in buffalo from slaughter house in Al-Najaf city of Iraq

Rashaa Ateaa; Mansour Ali Alkhaled

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 251-258
DOI: 10.33899/ijvs.2019.162882

Babesia is one of hemoprotozoan parasite transmitted by arthropod vectors which responsible for causing of Babesiosis disease in bovine worldwide. The present study was designed for microscopic identification, molecular, and phylogenetic analysis of Babesia species in buffalo from slaughter house in Al-Najaf city of Iraq. The study performed in three months of summer season (August into September 2017) and animals ages and sex were included in this study. The direct microscopic prevalence results were show highest prevalence of haemoprotozoa prevalence at Babesia sp. 45.74%. The prevalence of Babesia sp. related to animal sex, were show in male 43.48% and female was 52%, with non-significant differences. The Prevalence of Babesia sp. related to age were show 12.50%, 92.86% and 30% in young, adult and old age respectively with significant differences (P<0.05). The prevalence of Babesia sp. related to month of study were show. 28.57%, 62.50% and 42.86 in August, September and October respectively and with non-significant differences. Molecular study results were based on PCR and DNA sequencing method by phylogenetic tree analysis (MEGA 6.0) and NCBI-BLAST Homology Sequence Identity to differentiation Babesia species typing. The Babesia species prevalence results were show identified two Babesia species, high prevalence of Babesia bovis (38.30%) were closed related to NCBI-Blast Babesia bovis (HQ264126.1) with homology sequence identity 97-100% and Babesia bigemina 7.45% were closed related to NCBI-Blast Babesia bigemina (KU206291.1) with homology sequence identity 95-99%, then 43 Babesia species includes (B. bovis and B. bigemina) were submitted into NCBI-Genbank and provided accession numbers (MH503811-MH503853). In conclusion, this study concluded that Phylogenetic tree and homology sequences identity was show accurate in differentiation of Babesia species, and these species can be isolated at from local water buffalo from slaughter house in Al-Najaf city, of Iraq.

Application of magnetic technology in local quail house and hatchery on performance, reproductive and physiological traits under heat stress

Mahbuba Mustafa

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 259-266
DOI: 10.33899/ijvs.2019.162897

Using magnetic technology has shown major potentials in different fields especially agriculture. Two experiments were conducted to investigate the using of magnetic technology application in local quail house and hatchery on productive and physiological traits under heat stress condition. Results revealed that quail when served 0.2 T magnetically treated water (MTW) were significantly (P≤0.05) better in most productive, reproductive characteristics in the 1st experiment: eggs production, water consumption, egg weight, shell strength, estrogen conc., also improved FCR in females, all semen quality and testosterone conc. in males. Also, total RBCs count, thrombocyte, blood pH, blood viscosity, plasma viscosity, blood iron (Fe), copper (Cu), calcium (Ca), thyroxin hormone and total count of Lactobacillus spp. in small intestine. However, reduction in dead sperm, abnormal acrosome, corticosterone conc., body temperature, blood clotting time, E. coli and fungi counts in both sexes. In the 2nd experiment using of magnetic technology on embryonic development traits of local quails incubating eggs that collected from experiment 1 were significantly (P≤0.05) higher in the most characteristics at the group of 0.2 T MTW as fertility, hatchability, chicks weight, immune status of hatched chicks. While reduction in dead embryo and heterophil-to- lymphocyte (H/L) ratio. Finally, the results of 0.2 T MTW achieved superiorly gains compared with the other groups.

Analyses of mycotoxins in broiler’s local and imported feeds

N.M. Bibani; Z.K. Khidhir; A.S. Shaker; S.M. Kirkuki; S.M. Abdulateef

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 267-271
DOI: 10.33899/ijvs.2019.162994

In the present study 40 samples of broiler feeds that obtained from commercial feed manufacturing company (concerning 21 from Iraqi manufactures and 19 from the Iranian manufactures) were analyzed for the incidence of Aflatoxins, Ochratoxin, T-2, Fumonisins, and Zearalenone mycotoxins. The result has shown that AF was detected in 16 samples 76.19% of the Iraqi manufactures and 16 samples 84.21% from the Iranian manufactures, in concentrations ranging from 1.00-23.00 μg/ kg, and 1.00-2.00 μg/ kg respectively. Prevalence of AF contamination revealed a significant association (P<0.05) between the Iraqi and Iranian manufactures, the highest prevalence was observed in Iranian samples 84.21%, whereas it least in Iraqi samples 76.19%. OCHRA toxin was detected in 21 samples 100% of the Iraqi manufactures and 19 samples 100% from the Iranian manufactures, in concentration ranging from 3.00-4.00 μg/ kg, and (2.00 – 4.00) μg/ kg respectively. prevalence of OCHRA contamination revealed a non-significant (P>0.05) between the Iraqi and Iranian manufactures. T2 toxin was detected in 21 samples (100%) of the Iraqi manufactures and 19 samples (100%) from the Iranian manufactures, in concentration ranging from (2.00 – 16.00) μg/ kg, and (2.00 – 23.00) μg/ kg respectively. Prevalence of T2 contamination revealed a significant association (P<0.05) between the Iraqi and Iranian manufactures FUM3 toxin was detected in 21 samples (100%) of the Iraqi manufactures and 19 samples (100%) from the Iranian manufactures, in concentration ranging from (0.05 – 5.00) μg/ kg, and (0.10 – 2.50) μg/ kg respectively. Prevalence of FUM3 contamination revealed a significant association (P<0.05) between the Iraqi and Iranian manufactures, ZEAR3 toxin was detected in 15 samples (71.42%) of the Iraqi manufactures and 19 samples (100%) from the Iranian manufactures, in concentration ranging from (2.00 – 76.00) μg/ kg, and (1.00 – 41.00) μg/ kg respectively. Prevalence of ZEAR3 contamination revealed a significant association (P<0.05) between the Iraqi and Iranian manufactures, the highest prevalence was observed in Iranian samples (100%), whereas it least in Iraqi samples (71.42%).

Analgesic effect of silymarin in chicks

Ahmed Salah Naser; yasser albadrany

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 273-276
DOI: 10.33899/ijvs.2019.162906

There were no studies about the analgesic effect of silymarin in the chicken. This study examined antinociceptive effect of silymarin given intraperitonially in 7-9 day-old chicks. The median effective dose of silymarin for the induction of analgesia to electric stimulation in the chicks was 65.3 mg/kg. Silymarin at 60, 120 and 240 mg/kg revealed analgesic effect to electric stimulation in chicks in dose dependent manner in comparison with the control group. The analgesic effect of silymarin at 120 and 240 mg/kg started at 15 min after injection and lasted after over 120 min of injection were as silymarin at 60 mg/kg the analgesic effect started at 15 min after injection and declined before 120 min of injection. The peak of analgesic effect for 60, 120 and 240 mg/kg were at 60 min after injection. These results indicate that silymarin have an analgesic property in the chicks model.

Structural study of uterine tubes of the rabbit (Oryctolagus cuniculus) at different postnatal periods

Faeq Jabar Al-Saffar; Massarat Almayahi

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 277-288
DOI: 10.33899/ijvs.2019.162911

This study was designed to investigate the histomorphological changes in the uterine tubes of the local rabbits during three different periods of ages that were kitten, immature and mature stages of life. For such study, twenty-four female rabbits were used. Procedure of routine histological technique was performed to prepare histological sections by using general and special histochemical stains. Gross findings revealed the presence of bilateral uterine tubes which comprised thick short coiled preampulla, thin long ampulla and the isthmus which was shortest and straight region joined to the uterus. Microscopically three regions were identified according to the differences in their histological characteristic features. Data revealed that the wall of uterine tubes in kittens was not fully developed, whereas in immature does, global histological and histochemical changes were identified compared to kittens while mature does indicated critical role of the post weaning period on the development of the animals. Changes were specially recognized by histochemical procedure which showed strong positive reactions especially isthmus which obviously reacted with PAS, Ab, combined PAS-AB. In conclusion, current data showed unique differences of the uterine tubes in the local rabbits compared to other mammals especially those of rodentia species.

A comparative study between kessler suture versus polypropylene mesh implantation to repair tenotomized common calcaneal tendon in rabbits

Saddam Humadi

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 289-296
DOI: 10.33899/ijvs.2019.162907

The present study is assigned to throw the light on the benefit of using Kessler suture compared with polypropylene mesh implantation in healing of induced tenotomy of common calcaneal tendon in a rabbit model based on clinico-histopathological evaluation. Twenty adults New Zealand white male rabbits were used for this clinical prospective study divided equally and randomly into two groups. The first group, (suture group n=10) and the second group (mesh group n=10). Under the effect of general anesthesia, longitudinal skin incision over the common calcaneal tendon was made, and the skin flap was reflected. The tendon was isolated by blunt dissection from the surrounding tissue, with small curved forceps. Simulation of a rabbit’s common calcaneal tendon rupture was made by sharp transvers incision with scalpel. The first group repair with (Kessler Suture) using polypropylene suture size 4.0. In contrast, the second group repair with (polypropylene mesh implantation) after initial suturing of the cut ends of tendon by two simple interrupted stitches using polyglyconate suture size 4.0. The surgical skin wounds were re-stitched by simple interrupted pattern with 3-0 silk. After clinical follow-up of the treatment rabbits, certain secondary health problems were encountered represented by swelling of operative site and lameness. Grossly, adhesions were noticed between the tendon and subcutaneous tissue in rabbits of both groups. Microscopical examination reflected presence of inflammatory cells perivascular edema and few capillaries at two months. At the end of experiment there were perfect orientation and organization of collagen fibers in mesh group in comparing with suture group. Based on the results obtain from this study, it seemed that both groups reflected best outcome in healing of operated tendons with superiority of mesh group in comparing with suture group.

Assessment the level of some heavy metal in vital body fluids and blood parameters in lactating Awassi ewes

Ashwaq Ahmed Hassan; Rana amer asim Al Suleman agha; ALI SAEED HAMMOODI AL-CHALABI

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 297-301
DOI: 10.33899/ijvs.2019.162908

The aim of the study was to estimate the level of cadmium, zinc and oxidative stress parameters in serum and milk as well as the blood constituents in local at the age of 3-4 years during the spring and summer seasons. Blood and milk samples were collected from 30 ewes (15 each season). The blood samples were allocated to into two tubes, for complete blood profile study and for biochemical analysis. Milk was used for biochemical tests. Blood analysis showed a significant difference between some blood values during the two seasons represented by significant increase of total white blood number, haemoglobin concentration and packed cell volume in circulating blood of lactating ewes during summer season compared with spring season. Red blood cells number and mean corpuscular haemoglobin concentration did not change among the two seasons. Both mean corpuscular volume and mean corpuscular haemoglobin value of lactating ewe increased significantly during spring season with respect to the summer season. The serum glutathione level showed a significant elevation during spring season compared with the summer season, while, the level of malonaldehyde and peroxynitrite free radicle remain constant during the study period. There were no significant changes in the level of cadmium in serum and milk of examined ewes, while the serum level of zinc significantly higher during the spring compared to the summer season and milk zinc level remain stable during both seasons. The study concluded variations in haematological, oxidative stress biomarkers, serum and milk concentration of Zn and Cd according to lactation season in ewes.

Detection on ectoparasites on small ruminants and their impact on the tanning industry in Sulaimani province

Bahzad Mustafa

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 303-309
DOI: 10.33899/ijvs.2019.162995

The study was carried out through the clinical examination on the skins of sheep and goats to identify the quality and processed in the tanneries. Parasitic skin diseases caused by ectoparasites such as mange mites, lice, and ticks are among these threats resulting in a serious economic loss to the tanning industry and the country as a whole. Out of 38066 sheep and 9889 goats examined in Sulaimani slaughterhouse in spring season 2017, shows statistically significant P<0.05 between sheep and goats prevalence; 9.35% and 7.43% respectively with one or more ectoparasites. Three different of ectoparasites were infested in both sheep and goats namely; hard tick, lice and mite. Five species of hard tick (Ixodidae) were identified in sheep and goats as follows; Boophilus spp 3.68% and 14.16%, Rhipicephalus sanguineous 12.47% and 28.63%, Rhipicephalus turanicus 7.50% and 15.96%, Hyalomma a. anatolicum 11.90% and 31.30%, Hyalomma marginatum 9.26% and 9.95%) respectively. Two species of lice were identified in sheep namely, Damalinia ovis 17.74% and Linognathus stenopsis 13.63%, and D. caprae 10.97% and L. stenopsis 6.22% on goats. Highly infested were identified with mite on sheep include Psarcoptic scabiei 13.83% and Psoroptic ovies 9.98%, while only one species founded in this study in goats namely, Psarcoptic scabiei 22.79%. The overall number of skins refused to tanning industry 164 (4.61%) in sheep and 116 (15.78%) in goat. The difference in the prevalence of skin disease infestation between refused skin in both sheep and goats were statistically significant in ruminants (P<0.05). This paper deals with major skin defects in occurrence by ectoparasites sheep and goats in Sulaimani province.

The histological changes induced by cytarabine on rabbits kidneys (with and without vitamin E administration)

Saif Al-Jammas; ayad Al-Sarraj

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 311-316
DOI: 10.33899/ijvs.2019.162910

The present study was designed to determine the histological changes induced by Cytarabine drug on the structure of rabbits kidneys and the protective effects of vitamin E on these histological changes. The treated group with daily intraperitoneal dose of (50 mg/kg body weight) of Cytarabine alone, showed a massive histological changes represented by renal tubular necrosis, glomerular atrophy and enlarged urinary spaces (widening of Bowman’s spaces), infiltration of lymphocytes and macrophages within interstitium of the cortex, formation of hyaline cast in some of the tubular lumens as well as fibrosis and hemorrhage in the cortex were also observed. While the group that received a protective (800 IU of vitamin E) prior to each Cytarabine injection showed a significant improvement that represented by focal regions in the cortex with a normal renal tubules except for a cloudy cell swelling which is a reversible injury, also neither cortical hemorrhage nor hyaline cast formation were seen, in addition to presence of focal areas of normal glomeruli. The present study suggests that vitamin E is an effective chemo-protective agent against nephrotoxicity when used prior to each Cytarabine administration.

A comparison between two different biomaterials for treatment of tracheal defect in dogs

Osama Al-Hyani; Abd Al-Haleem Al-Hasan

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 317-327
DOI: 10.33899/ijvs.2019.162883

This study was designed to evaluate the healing process of tracheal cartilage defect after implantation of two different biomaterials in dogs. Eighteen adult dogs from both sexes were used. They were divided into 2 equal groups. A window shape defect of about 3cm x 2cm was induced in the cervical part of three adjacent tracheal rings with their annular ligaments. In group one, the defect was closed with a small strip of ear cartilage (auto graft) from the same animal, while in the second group, calf's pericardium scaffold (xeno graft) was used to close the defect. All the animals were clinically monitored postoperatively, while gross pathological changes and biopsy collections for all animals was performed at 15, 30, 60 postoperative days. No postoperative clinical complications were recorded and all were looking healthy and resuming food and water intake normally. The gross pathological results revealed rapid occlusion to the trachea especially in first group and animals survived without any respiratory complications. The histological features in both groups were represented by newly granulation tissue formation and hyaline cartilage regeneration. The new hyaline cartilage cells formation was showed faster in group 1 rather than group 2. In conclusion, both grafts can be used for closing a tracheal defect in dogs with the priority of ear cartilage implantation.

Different vectors used to transform and clone of nonstructural NS1 gene of Influenza B in Escherichia coli

Ali Adel Dawood

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 329-333
DOI: 10.33899/ijvs.2019.162964

Flu is a highly contagious and common illness caused by influenza A, B, and C viruses. The aim of the present study was to investigate the transformation and cloning of NS1B gene with pET-32a, pET-32b and pQE-81L in Escherichia coli BL21(DE3) and DH5α. pUC57-NS1B synthetic gene was transform and clone in Escherichia coli BL21(DE3). Isolation, single digestion and ligation with pET-32b using HindIII restriction enzyme. Amplification of recombinant DNA was done with conventional PCR after transformation. Screening with IPTG of colonies. Gel electrophoresis was done for each step of cloning after isolation. Isolation, double digestion and ligation with pET-32a and pQE-81L using SacI, PstI and HindIII respectively.Recombinant DNA was attempted to be transformed into E. coli strains BL21 (DE3) and DH5α. pUC57 plasmid carrying NS1B gene was successful transformed and isolated from E. coli BL21 (DE3). Designed primers used for PCR of NS1B showed successful amplification. First screening of pET-32b-NS1B colonies using white/blue method, cloning NS1B into pET-32b using single restriction digestion with HindIII, pET-32a using double restriction digestion with SacI and HindIII and pQE-81L using double restriction digestion with PstI and HindIII gave unexpected result. This result may relate to re-ligation of digested vector for single digestion and uncompleted digestion for vectors of double restriction digestion. Current study has suggested that recombinant NS1B gene can be cloned using single digestion with other expression vectors.

Histological and antimicrobial effects of tramadol infiltration on incisional oral mucosal wound healing in rabbits

F.A. Al-Mashhadane; E.A. Mustafa; G.A. Taqa

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 335-340
DOI: 10.33899/ijvs.2019.162996

The present study compared effects of tramadol with lidocaine on healing processes of incisional intraoral mucosal wounds in rabbits and to study the antimicrobial effect of tramadol. For this purpose, fifteen apparently healthy male rabbits of 5-6 months old were used. They were classified into three groups, first group treated with 0.5 ml distilled water infiltration as control (n=5), second group treated with 0.5 ml tramadol infiltration in dose of 100 mg/2 ml (n=5), and third group treated with 0.5 ml of 2% vasoconstrictor-free lidocaine infiltration, then an incision was done to the buccal mucosa of all animals and they slaughtered on day 10 and their wounds were evaluated by histopathologic for presence of edema, vascularity, and inflammatory cells. For antimicrobial effect, swabs from tramadol group were taken from sites of wounds before incision and other swabs were taken after surgical incision on the first day, third day and seventh day, then the colony forming units (CFU)/ml of microorganisms were counted. The present study found that the infiltration with tramadol in dose of 100mg/2ml result in new epithelialization and wound healing occurred faster than infiltration with lidocaine 2% group also this study found that tramadol has significant decrease in the means of colony forming units of oral microorganisms. This study demonstrated that tramadol accelerated stages of buccal wound healing faster than lidocaine in rabbits.

Nad1 gene analysis of Echinococcus granulosus from sheep in Aqrah city, Iraq

R.N. Hamoo; nashaat Ghalib Mustafa; S.A. Abdulraheem

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 341-345
DOI: 10.33899/ijvs.2019.162965

Echinococcus granulosus (E. granulosus) is a dog tapeworm cestoda; it is larval stage responsible to cystic echinococcosis, one of the most common and dangerous worldwide zoonotic parasitic disease. The aim of this study was the molecular identification of the local strain of E. granulosus isolated from sheep liver slaughtered in the principal abattoir of Aqrah city, Northern of Iraq during Jun-Nov. 2017. In this study, 37 sheep liver infected by E. granulosus, 12 of high DNA purity fertile (have protoscolices) cyst of them were considered. A molecular study conducted on the mitochondrial NADH dehydrogenase 1 (nad1) gene. Results demonstrated that E. granulosus isolates were sheep strain (G1) genotype, with fascinating highly corresponding 95% and 96% to global isolates, particularly to north African and Mediterranean countries, by employing phylogenetic tree analysis. So, the isolates of our project were deposited in Genbank (accession No. MG792129). This study findings provide that the local isolates of E. granulosus from sheep liver in Aqrah city, Northern of Iraq are loyally equivalent to global strains and isolates, in addition, nad1 gene considers a perfect biomarker in a molecular identification and phylogenetic study of this parasite.

Detection of ectoparasites in different birds

Manal Hammadi Hasan

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 37-41
DOI: 10.33899/ijvs.2019.162896

The study was conducted to detect the ectoparasites infestation in chickens, Domestic pigeons Columba livia domestica and turkeys in different areas of Mosul city by survey examining of 80 birds of chickens, 85 birds of pigeons and 50 birds of turkeys, of both sexes. Results show the infestation rates with ectoparasites in chickens, pigeons and turkeys, were 41.3%,37.6% and 36% respectively. Results show that the chickens were infested with three types of ectoparasites, two species of lice are Menacanthus stramineus, Gniocotes gallinae and soft ticks Argas persicus the infestation rates were 63.6%, 24.2% and 36.4% respectively. The highest infestation rate with lice in chicken was 70% in Spring season and the lowest rate 5% in Summer season while the infestation rates in Autumn and Winter were convergent, there was 44.4% and 45.5% respectively. The results show that the pigeons were infested with two types of ectoparasites, one species of lice is Columbicola columbae and soft ticks is Argas persicus, the rates were 87.5%,18.8% respectively. The infestations were individual and double. In turkeys the results record one lice species Goniocotes gallinae, the infestation rates were 36%. The male and female were infested with rates 27.3% and 42.9% respectively.