This study was conducted to isolate and diagnose mycobacteria species from local freshwater fish samples collected from different markers in Ninevah province. Seventy samples were examined by staining and study of cultural characteristic of colonial growth, two mycobacteria species were isolated from 10 (14.3%) samples, seven (10%) of them were M. fortuitum, while 3 (4.3%) were M. marinum from different species of fishes belongs to family Cyprinidae which includes the genus of Barbus, B. sharpeyi (2%), B. grypus (3%), B. xantnopterus (2%) and family siluridae includes the genus Silurus glanis (1%), and the genus of Carp, Cyprinus carpio (2%).