Microscopic and PCR-based detection of Babesia bovis and Babesia bigemina in camel females from Al-Diwaniyah Province, Iraq
Iraqi Journal of Veterinary Sciences,
This study was carried out to identify the etiological pathogens responsible of protozoal-like disease conditions occurred in camel females from Al-Diwaniyah Province, Iraq. For this reason, 125 camel females (one blood sample per animal) that showed signs of weakness and pale mucus membranes were considered for the study. The samples of blood were explored microscopically and via the use of a polymerase chain reaction (PCR) method that the targeted glutamine-dependent carbamoyl phosphate synthase (CPSII) gene for identifying Babesia bovis and 18S rRNA gene for detecting B. bigemina. The results of the microscopic technique uncovered the occurrence of babesia spp. in 76 (60.8%) of the examined samples, which encourage the use of the PCR to identify the protozoal species. The PCR findings demonstrated that B. bovis and B. bigemina were detected in 8 (8.9%) and 11 (12.22%), respectively, of the positive microscopic samples. The study findings reveal that weakness and paleness of mucus membranes in camel females can be attributed to the presence of infections by blood protozoa, mainly Babesia bovis and Babesia bigemina.
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