HISTOCHEMICAL STUDY OF THE HEPATIC METACESTODES IN INFECTED SHEEP WITH HYDATIDOSIS
Abstract
This study aimed detection the liver histopathological changes of hydatid cysts sheep infestation. Seventeen infested liver's sheep with hydatid cysts were gathered from the local butchers. The specimens sectioned, processed, and stained with hematoxylin and eosin and some special stains such as Gomori's aldehyde fuschin, Van Gieson, Toluidine blue, Alcian blue, Periodic Acid Schiff (PAS) and Mansso's trichrome. The results of hematoxylin and eosin stain of infected groups revealed presents of the laminated membrane of hydatid cyst in a different area of hepatic tissue surrounding by infiltration of inflammatory cells, and increase fibrous tissue and between severe necrotic hepatic tissue. The special stains firstly as in Gomori's aldehyde fuschin stain revealed the protoscolex in green colour and elastic fibres of connective tissue surrounding hydatid cyst with its laminated membrane in purple colour. Van Gieson stain showed dark brown-purple colour of the elastic fibres reduplication of connective tissue surrounding hydatid cyst, while Toluidine blue stain sections revealed the dark blue stain of acidic components of hydatid cyst laminated membrane and necrotic hepatocytes. Alcian blue stain revealed the blue colour reactivity of the proteoglycans of the connective tissue surrounding hydatid cyst. PAS reaction stain revealed the magenta colour reaction of the intact hepatocytes in the control group comparing with depletion pf magenta colour in necrotic hepatocytes surrounding hydatid cyst. Masson's trichrome stain revealed increased collagen fibres of the connective tissue. The findings revealed that infection with hydatid cysts causes a variety of histopathological alterations that appeared through several tissue stains
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