Molecular identification of Diarrheagenic Escherichia coli (DEC) isolated from meat and meat products
AbstractThe aim of study is to diagnose diarrheagenic Escherichia coli from meat and their products by conventional polymerase chain reaction technique using uidA gene for confirmation the bacterial isolated as E. coli and used multiplex PCR technique to detect the virulence genes of these bacteria by using two groups of primers (stx1, stx2, aggR, esth, eae) and (invE, daaC, estp, elt, bfpA). In this study these primers applied on a total of 100 E.coli strain isolated from 782 samples of meat and meat products included fresh meat , minced meat ,burger , pastterma and chicken in the period of February to November 2020.
The results showed that all isolates of Escherichia coli were positive to gene uidA and showed bands with a molecular weight of 147 base pairs. also in this study, we detect 95 ( 12.15%) pathogenic species related to virulence genes by using multiplex PCR . The highest percentage of pathotype is ETEC , at a rate of 46.32% and the lowest percentage is due to the DAEC type at a rate of 1.05% , while the percentage of the other pathotypes were 20.05, 14.74, 6.32, 6.32 and 5.26% for each of STEC , EHEC , aEPEC , EAEC and EIEC respectively. The high contamination rate with DEC that was seen in this study is associated by poor hygiene conditions during the slaughter process, and poor food storage in the shops, which may pose societal health risks to people when consumption of these products .
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