In vitro study of Curcumin Calcium Carbonate Phosphate Nanoparticles (Curcumin-NPs) impacts on the Meriz goat’s coccidian Oocysts
AbstractNanoparticles biosynthesis have an essential and increased role in the delivery of medical compounds.
Calcium carbonate phosphate nanoparticles (CaCO3-NPs) were prepared as a stabilized amorphous and incorporated with herbal curcumin extract as an anticoccidial agent In Vitro. CaCO3 – NPs was tested against local Meriz goat coccidian oocysts.
Concentrations were used (2, 4, 8, 16, 30 and 50 mg/ml) shows oocysticidal effects (Sporulation inhibition) and sporocystidal effects (concentration of 100, 200, 400, 800 and 1000µg).
Sporulation inhibition assay was used for 24 and 48 hours. Results of significant oocysticidal effect (P=0.001) seen in concentration of 30 - 50 mg/ml and able to inhibited the sporulation of Meriz coccidian parasite oocysts at a rate of 92.54 ± 3.51%.
The sporocysticidal effect also significant (P=0.001) with curcumin nanoparticles concentration of 400-1000 µg/ml with rate of 98.1 ± 2.11%.
Stability of prepared curcumin nanoparticles was examined against various pH levels (4.01, 7 and 9.21) at multiple temperatures (4, 25, 60 and 100 oC). Investigation after 1, 6, 12 and 24 hrs. of treatment. Stability was assessed by spectrophotometric indicated significant reductions for pH 4 and 9 after one hour of treatment (P=0.001) as well as at the temperature of 60 oC and 100 oC after 12-24 hours of treatment (P=0.001).
These results reflect promising hopes of exploiting CaCO3 curcumin nanoparticles for the eradication of coccidiosis as they are composed of and prepared from natural substances.
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