A study of primary ossification centers in the hind limbs of Awasi sheep fetuses by double stains method and radiography.
AbstractForty Local Awasi sheep fetuses were collected from several areas in Ninevah Governorate, further than local slaughterhouse. Their estimated ages calculated depending on crown-rump length with the aid of Richardson formula. The study aimed to detect the site and the time of appearance of ossification centers in the hind limb bones of Awasi sheep fetuses by using modified double staining method to demonstrate cartilage and bone , or by using radiography. The first ossification centers appeared in the hind limb bones was the primary center of the tibia and femur ,metatarsus with ilium then ischium at 45 , 46 , 48, 50-52 days of fetal age respectively. The ossification centers of pes phalanges appeared in the following sequence ; distal phalanx ,followed by the proximal phalanx and end by middle phalanx , at 58 ,60, 64 days respectively. Ossification centers of tarsal bones began to appear in calcaneus at 64 day , talus at 77 day , central & 4th tarsal bones at 96 day ,, followed by centers of 3rd & 2nd tarsal bones at 100 day while, the center of 1st tarsal bone appeared lastly at 105 day.Femur , tibia and metatarsus studied statistically by follow up the relative increase of their total lengths and length of their ossified part of diaphysis , the results show high significant variance in their growth and ossification of the three bones in first studied period (46-60 days) in comparison with the next sixth successive fortnightly periods
- The first primary ossification center appears in diaphysis of tibia which represent the longest bone of hind limb of Awasi sheep fetus.
- Ossification centers of phalanges begin to appear in distal phalanx at 58 day and end in middle phalanx at 64 days.
- The first tarsal bone begins to ossify is the calcaneus at 64 days, while the 1st tarsal bone is the last one, begin to ossify at 105 days.
- There was significant relative increase of the ossification through the diaphysis of three long bones femur, tibia and metatarsus, which seem very high at 7th and 8th weeks of gestation.
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