Application of species-specific polymerase chain reaction technique depending on cytochrome b gene for beef authentication
Iraqi Journal of Veterinary Sciences,
2012, Volume 26, Issue Suppl. III, Pages 193-196
AbstractThe study was conducted on imported beef meat (Indian, Brazilian and Australian), and spacement of minced beef meat (Al-jnobi, Al-sharabi) and Al-frezian collected from local grocery stores for beef authentication to differentiate them from others meats in order to protect consumer economically through applying Species-specific Polymerase Chain Reaction technique using n cytochrome b gene. Results of this study indicated that Species-specific PCR technique was very sensitive and highly specific for the identification of the meat type, also it was found that the designed primer on mitochondrial cyt b gene of beef proved practically proved to be applied on local and imported types of meat of different breeds, since gives the same molecular weight 365 bp in all the same bands. From five minced beef samples, one sample was pure beef, other one contained no beef at all, while the remaining three samples were mixed meat of beef, buffalo, goat and mutton.
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