Molecular identification of new circulating Hyalomma asiaticum asiaticum in the Sheep and goats in Duhok Province-Iraq
Ticks are obligate ectoparsite that infest both animal and human and causing many diseases, due to they act as a vector of some pathogens including: protozoa, bacteria and virus. There are about 877-878 tick species; most of these are under the two famous families including: Ixodidae and Argasidae. Because there was no such study done on identification of tick species by PCR technique in Kurdistan and particularly in Duhok Governorate, therefore present study was done to identify tick species by using molecular study by using of 16S rRNA and DNA sequencing. About 1000 ticks were collected from both sheep and goat respectively (500 and 500), form Duhok Governorate including: Barwaria, Zakho, Sumeil, Mangeshik, Sersin, Shekhan and Akre, Iraqi Kurdistan, between May and June 2016, between April and June 2017. The result found during this study were six species under two genera of the hard ticks were identified by molecular study and sequencing including: three species were under the genus Hyalomma and three species were under the genus Rhipiciphalus that infect small ruminants in Duhok Governorate from these species a new species under the Hylomma genra (Hyalomma asiatium asiaticum) with accession number (MN594484), was first time reported in Duhok Governorat, Iraqi and in Kurdistan. Also phylogenetic tree was constructed depend on the 16S rRNA. More advanced molecular study are need such as the PCR – RFLP method will be more accurate molecular tool for ticks differentiating of Duhok and will be helpful in epidemiological and geographical distribution .
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