Evaluation the safety and synergistic effect of NiFe2O4 Nanoparticles with antibiotic against pseudomonas aeruginosa
AbstractAntimicrobial resistance currently impedes and threatens effective prevention and treatment of the continually expanding range of infections caused by bacteria. This study aimed to identify the bacterial causes of wound infection among animals and using of antibiotic/nanoparticles mixture as a new attempt for the treatment of wound infection induced in rats. For this purpose, 112 wound infection cases in different animal types (36 sheep, 21 goats, 12 cows, 4 horses, 8 dogs, 9 rabbits, 7 genies pigs, 15 rats) were studied for bacterial isolation. The predominant bacterial isolate (Pseudomonas aeruginosa) were tested for its sensitivity to antibiotics and nanoparticles (CoFe2O4 and NiFe2O4) in vitro using MIC method. Also wound infection induced in rats and the effect of nanoparticles/antibiotics mixture were tested in vivo.
The results showed that pseudomonas aeruginosa was the predominant bacterial type that caused wound infection. The MIC of NiFe2O4 and CoFe2O4 nanoparticles were 32 µg /ml and 16 µg /ml respectively. A clear synergistic effect of antibiotic/ nanoparticles as antibacterial were noticed which appear as decrease in MIC and increase of inhibitory diameter zone. According to result of RAPD test, nanoparticles effect on genetic material of P. aeruginosa observed as appearance/disappearance of bands, increase in thickness and clarity of bands.
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