Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province
Iraqi Journal of Veterinary Sciences,
This study were conducted in Al-Diwaniya province, in south Iraq during the period from February to July (2019) to determine the rate of infection of Cryptosporidium parvum in domestic chicken, study the effect of some epidemiological factors such as sex and months on the rate of infection, addition to the molecular Identification of Cryptococcus parvum by amplification HSP70 gene by conventional PCR for first time in Iraq. Number of collected fecal sample was (210) from domestic chicken and processed by Ziehl-Neelsen method. The results of the microscopic examination showed that 108 (51.4 %) out of 210 fecal samples were infected with Cryptosporidium spp. The statistical analysis founded no marked difference in prevalence of infection between sexes. Significant difference (P<0.05) was recorded between infection rate during the months of the study and higher prevalence of infection rate was observed in March (11.9%), while lowest infection rate was observed in July (5.23%). and June (5.23%). Genomic DNA was Genomic DNA was extracted from 108 fecal samples and HSP70 gene for C. parvum was amplified by polymerase chain reaction (PCR). PCR technique is showed that out of 108 fecal samples (21.3%) were positive for C.parvum.
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