Molecular detection of pathogenic Entamoeba spp. and Genotyping of Entamoeba histolytica in Hemorrhagic diarrhea samples of humans, cows and sheep.
The present study was carried out to detect the genotype of Entamoeba histolytica that found in human fecal specimens and animals feces with Haemorrgic diarrhea by amplifying the SREHP gene, using Real Time PCR technique, Cyber green dye and by fusion curve analysis. The study also included molecular detection of amoebic parasite species using PCR technology, this technique based on 18S rRNA genes responsible for the diagnosis of Entamoeba histolytica; Entamoeba dispar and Entamoeba bovis. The study recorded presence of parasites (Entamoeba histolytica; Entamoeba dispar; Entamoeba bovis) with total infection rates (82.9% ; 26.8 % ;4.9 % ), respectively. The study revealed the presence of Entamoeba histolytica parasite in five different genotypes (I, II, III, IV, V) with rate presence (9.75% ; 53.65 % ; 19.5% ; 9.75% ; 7.3%), respectively. In conclusion, There are five genotype of Entamoeba histolytica , in human and animals , most of these genotype may be infect any host , Entamoeba bovis was record in sheep and cows .
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