Detection of hbl complex genes in Bacillus cereus isolated from cow raw milk in northwest of Iran
Iraqi Journal of Veterinary Sciences,
Bacillus cereus is a gram positive and spore-forming bacterium which is widely distributed in nature. It also has been known as a major food borne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type. The aim of this study was detection of hbl complex genes in B. cereus isolated from cow raw milk in northwest of Iran. 120 B. cereus strains isolated from cow raw milk that already had been identified phenotypically, were assessed for molecular confirmation by PCR method. Then, they were evaluated for determination of presence of hbl genes complex by specific primers. Of 120 B. cereus strains, 117 strains were confirmed molecularly. The frequency of hblA, hblC and hblD genes were reported 105(89.7%), 102(87.1%) and 102(87.1%), respectively. 99 strains (84.6%) harboured 3 tested genes simultaneously. 12 B. cereus strains (10.25%) lacked these genes. The results of current study showed that B. cereus strains isolated from raw milk have high potential in causing diarrhea poisoning and therefore the use of procedures to reduce the bacterial contamination during the processing of dairy product is required.
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