Iraqi Journal of Veterinary Sciences

Abstract

Through this study 122 individual fecal samples was collected from dogs , with different ages ,sexes, and breeding management ( stray and pet house hold ). some dogs were with healthy appearances while others were suffered from diarrhea ,anorexia ,depression , dehydration. Infection was investigated by light microscope after flotation technique by using Sheather's sugar solution. if the sample gives positive to oocyst (H. heydorni or other relative group),were mixed with potassium dichromate solution a rate of 2.5% for the stimulation of sporulation. DNA extraction was done using 2% sarcosyl ,pronase E followed by phenol/chloroform extraction then precipitation attempt using ethanol. DNA amplification was attempt using H. heydorni primers JS4 and JS5 n microscope examination give 78 sample positive to the presence of oocyst , while polymerase chain reaction showed total percentage of infection with H.hydorni was 34.6% ( 27 case give positive reaction to PCR). Dogs less than 6 months of age show high infective rate (17.9%), dogs which suffer from gastrointestinal tract troubles (diarrhea , anorexia and dehydration) gave high infective rate (24.4%). Stray dogs gave high prevalence of infection to H .hydoeni (25.6%) when compared with pet house hold dogs which recorded (9%) percentage of infection. This study is the first recorded H.hydorni in dogs in Mosul city.