Detection of Listeria monocytogenes in common carp (Cyprinus carpio) Fish in Iraq
AbstractAim: There is alittle reports are available for detection of L. monocytogenes in fish in Iraq, however, the current study was undertaken to investigate the potential role of Listeria sp. in common carp fish in Baghdad province /Iraq.
Materials and Methods: A fresh raw common carp (Cyprinus carpio), thirty fish in total were purchased from fish sellers of various markets in Baghdad city. The viscera was removed and heamognized perior the bacterial isolation and identification that conducted by conventional culture method using Listeria selective media, biochemical tests and Vitek 2 Gram-positive (GP). Pathogenicity of isolates was in vivo applied by inoculated mice, targeting virulence associated genes used to detected the virulence genes and to confirmation the L. monocytogenes isolates. The L. monocytogenes isolates were tested for antimicrobial susceptibility by disk diffusion method, twelve types of antibiotics were chosen.
Results: Two isolates (6.67%) L. monocytogenes were identified from common carp fish visceras and the isolates were pathogenic in mice, L. monocytogenes virulence associated genes were detected in both isolates while L. innocua virulence associated genes not detected except Lin0372 was found in one isolate. The isolates were sensitive to 6 out of 13 antibacterial drugs including Chloramphencol, Methicillin, Imipenem, Ciprofloxacine, Amikacin and Ofloxacim.
Conclusion: our results suggesting that L. monocytogenes isolates have the potential role in public health hygienic in humans.
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