Using T cell Lymphokines to enhance the immune response against Newcastle disease in vaccinated broiler chickens under the effect of Mycotoxin
Iraqi Journal of Veterinary Sciences,
The current study aimed to reduce the toxic effect of Mycotoxins and enhance the immunity against Newcastle disease in broiler chickens by using lymphokines from hyperimmunized birds with salmonella typhymurium. The study included three stages, the first phase included isolating salmonella typhymurium from the infected birds and then determined the infected dose by injecting five groups of broiler chicks, each group included 5 chicks, the dose was 108, the second phase included after isolating the bacteria and determine the infected dose, was taken two groups of broiler chicks, each group included 20 chicks at one day, the first group was drinking orally three doses (7, 14 and 21) days. The second group not taken anything as considered a control group. At 35 days of age, T cell cells were collected from spleen of the infected bird, after it was destroyed and filtered (1500) rpm with centrifuge, and then grow T cell on RBM media and added Co-A to stimulate T cell to secretion the Lymphokines. Third and final phase of the study involved 250 broiler chicks at one day, divided into five groups each group 50 chicks, treated as follow: the first group was injected 0.5 ml intraperitoneal with Lymphokines at one day, after 30 minutes vaccinated with Newcastle (ND) vaccine (La Sota strain) through drinking water, the injection and the vaccine was repeated at 10 days.
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