Anticlastogenic properties of Quercus infectoria galls extract against DMBA induced genotoxicity in bone marrow cells of mice in vivo
AbstractThis study aimed to evaluate the aqueous extract of Quercus infectoria galls extract (QIGE) as anticlastogenic. The effect of QIGE was tested in mice (5 groups for each test) treated with 7, 12-dimethylbenz (a) anthracene (DMBA), the strong site-specific carcinogenic agent. In this study, the QIGE show no signs of toxicity, a single dose of DMBA (50 mg/kg) was injected intraperitoneally to Swiss albino mice caused a great increase in number of chromosomal aberrations, micronucleated polychromatic erythrocytes (MnPCEs) and reduction in the percentage of mitotic index (MI) (cytogenetic markers). Oral pre-treatment and post-treatment of QIGE for 14 days at dose 2 gm/kg b.w. daily to DMBA-treated animals greatly reduced in number of micronucleus formation, chromosomal abnormalities such as chromosomal break, chromatid breaks, ring chromosome, dicentric chromosome and fragments. Besides, mitotic index frequency increased comparing with the positive control. The data suggest that QIGE has potent anti-clastogenic effect against DMBA-induced genotoxicity in bone marrow cells of albino male mice and it may have a protective effect against the mutagenicity of the polynuclear aromatic hydrocarbons (PAH).
1.This study aimed to evaluate the anticlastogenic effect of the aqueous extract of Quercus infectoria galls extract (QIGE) against 7, 12-dimethylbenz (a) anthracene (DMBA).
2.Different QIGE doses of Quercus infectoria galls extract (QIGE) 2, 4, 6, 8, 10 and 12 gm/kg b.w. show no signs of toxicity; the recorded data suggested that the aqueous extract of QIG is safe.
3.A single dose of DMBA (50 mg/kg) caused a great increase in number of chromosomal aberrations, micronucleate polychromatic erythrocytes (MnPCEs) and reduction in the percentage of mitotic index (MI) in bone marrow cells of albino mice.
4.The present study found that the Oral pre-treatment and post-treatment of QIGE for 14 days at the dose 2 gm/kg b.w. daily to DMBA-treated animals reported inhibitory and protective effects on the cytotoxic and DNA damage induced by DMBA.
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