Uses of direct and indirect immuno-fluorescent techniques for demonstration of nematodes infection in sheep in Nineveh government
Iraqi Journal of Veterinary Sciences,
Volume 34, Issue 1, Pages 17-22
AbstractThis study was conducted for comparison between two different modern methods for the diagnosis of the nematode worms' infection in sheep we used acridine orange fluorochrome and specific staining kit in fecal samples. We examined 50 sheep fecal samples; the total infection rate was about 96% when was used lectin - fluorescein kit while the total infection rate was 74% when we used acridine orange fluorochrome technique. In conclusion of this study indicated that lectin - fluorescein kit is more effective than acridine orange fluorochrome in the diagnosis of nematode worms' eggs. Also, we conclude from this study the importance of using fluorescence - lectin kit technique in the diagnosis of nematodes eggs because they are distinguished by their speed, efficiency and accuracy, as they compare with the fecal culture technique to cultivate the larvae from eggs in order to diagnosed the type of nematodes eggs, the lectin - fluorescein kit technique is the first in Iraq.
- Bowman DD, Lynn RC, Eberhard ML. Georgi's parasitology for veterinarians. 8th ed. USA: Sanders Elsevier Science; 2003. 153-229p.
- Gessner T, Mayer U. Triarylmethane and diarylmethane dyes in Ullmann's encyclopedia of industrial chemistry. New York: Wiley-VCH Weinheim; 2002. http:// ki.se/ sites/ default /files/ gorankronvall as staining mini–review.pdf. doi: 10-1002/14356007.a27179
- Hemavani N, Chitinis D, Dixit DS, Asolkar MV. Acridine orange stained blood wet mounts for fluorescent detection of malaria. Indian J Pathol Microbiol. 1999;42:125-128.
- Keiser J, Utzinger J, Premji Z, Yamagata Y, Singer BH. Acridine orange for malaria diagnosis: Its diagnostic performance, its promotion and implementation in Tanzania, and the implications for malaria control. Ann Trop Med Parasiotol. 2002;957:643-654. DOI: 10.1179/000349802125001834
- Gainer JH. Demonstration of Anaplasma marginale with the fluorescent dye, acridine orange, comparisons with the complement fixation test and wright's stain. Am J Vet Res. 1961;22;882-886.
- Kawamoto F. Rapid diagnosis of malaria by Fluorescence microscopy. Lancet. 1991; 337:200-202.doi.org/10.1016/0140-6736(91)92159-Y
- Fripp PD, Mason PR, Super HA. Method for the detection of Trichomonas vaginalis using acridine orange. J Parasitol. 1975;61:966-967.
- Levett PN. A comparison of five methods for the detection of Trichomonas vaginalis in clinical specimens. Med Lab Sci. 1980;37:85-88.
- Shute GT, Sodeman TM. Identification of malaria parasites by fluorescence microscopy and acridine orange staining. Bull World Heal Org. 1973;485:591-596.
- Kong HH, Chung D. Comparison of acridine orange and giemsa stains for malaria diagnosis. Korean J Parasitol. 1995;334:391-394.
- Yoon E, Vail E, Sann L, Brass J. New staining technique for diagnosing Babesia species. Amer J Clin Pathol. 2015;144(Suppl 2,1):228. doi.org/10.1093/ajcp/144.suppl2.228
- Salih DA, El Hussein AM, Singla LD. Diagnostic approaches for tick-borne haemoparastic diseases in livestock. J Vet Med Anim Heal. 2015;72:45-56.DOI:10.5897/JVMAH2014.0345.
- Suleiman EG. Morphological and molecular study of Babesia spp. and isolation and diagnosis of tick vector in infected cattle in Mosul city [PhD dissertation]. Mosul: University of Mosul, College of Veterinary Medicine; 2018. 124-126 p.
- Suleiman EG, Altaee AF. The possibility of using acridine orange compared to giemsa stain in the diagnosis of parasite Babesia spp. in cattle. J Vet Med Sci. 2019;33(2):1-7. doi.org/10.33899/ijvs.2019.153870
- Silvestre A, Humbert JF. A molecular tool for species identification and benzimidazole resistance diagnosis in larval communities of small ruminant parasites. Exp Parasitol. 2000;95:271-276. doi.org/10.1006/expr.2000.4542
- Hamid R, Masood A. A dietary lectin as disease causing toxicants. Pakistan J Nutri. 2009;3:293-303.
- Colditz IG, Le LF, Hosse R. Use of lectin binding characteristics and identify gastrointestinal parasite eggs in faeces. Vet Parasitol. 2002;109:219-227doi.org/10.1016/S0304-4017(02)00013-4
- Foreyt WG. Veterinary parasitology: Reference manual. 5th ed. New York: Blackwell; 2001. 81-91 p.
- Thienpont D, Rochette F, Vanparijs O. Diagnosing helminthiasis through coprological examination. Belgium: Janssen Foundation Beerse; 1986. 30p.
- Lauer BA, Reller LB, Mirrett S. Comparison of acridine orange and gram stains for detection of microorganisms in cerebrospinal fluid and other clinical specimens. J Clin Microbiol. 1981;142:201-205.
- Umair S, McMurtry LW, Knight JS, Simpson HV. Use of fluorescent lectin binding to distinguish eggs of gastrointestinal nematode parasites of sheep. Vet Parasitol. 2015;217:76-80. https://doi.org/10.1016/j.vetpar.2015.12.029
- Ravindran R, Lakshmanan B, Sreekumar C, Dohn L, Gomathinayagam S, Mishra A, Tewar AK, Raod R. Acridine Orange staining for quick detection of blood parasites. J Vet Parasitol. 2007;211:85-86.
- Goldsmid JM, Rogers S. Preliminary report on the use of acridine orange O for the detection of Babesia canis in the blood. TST Cent Afr. 1977;232:35-36.
- Tarimo DS, Mpembeni R, Kawawa H, Mshana TC. Appraisal of the acridine orange method for rapid malaria diagnosis at three Tanzanian district hospital. East Afr Med J. 1998;75:504-507.
- Gay F, Boubacar T, Zanoni D, Danis M, Balnc AF. Direct acridine orange fluorescence examination of blood slides compared to current techniques for malaria diagnosis. Trans R Soc Trop Med Hyg. 1996;90:516-518.
- Hillrichs K, Schnieder T, Forbes AB, Simcock DC, Pedley K, Simpson HV. Use of fluorescent lectin binding to distinguish Teladorsagia circumcincta and Haemonchus contortus eggs third stage larvae and adult worm. Parasitol Res. 2012;110:449-458.
- Nordbring B. Action of a nematode-trapping fungus shows lectin-mediated host-microorganism interaction. Nature. 1979;281:477-479.
- Chaney A. Identification of internal parasites of sheep and goat [MSc thesis]. Houston: Honor College; 2012. 26 p.
- Article View: 120
- PDF Download: 172