Propagation of Salmonella enterica serovar typhimurium in embryonated chicken egg
Iraqi Journal of Veterinary Sciences,
2019, Volume 33, Issue 2, Pages 81-86
AbstractThe study included the injection of Salmonella enterica serovar typhimuriumisolated from starlings bird in embryonated chicken eggs. The eggs were divided into eight groups, each group contain 6 eggs. The G1 and G2 groups were injected with the sterile normal saline solution in the choriaollantoic membrane (CAM) and yolk sac as negative control. The group G3 and G4 injected by bacterial suspension at a concentration of 104 cfu/ml in the CAM and yolk sac while the G5 and G6 injected with bacterial concentration 106 cfu/ml in the CAM and yolk sac respectively. Finally, the G7 and G8 groups were injected with 108 cfu/ml of bacterial suspension in the CAM and yolk sac respectively. The results showed that the highest percentage of death in eggs embryos was 100% in the sixth group after 96 hours of injection. There was also a significant increase in the number of bacteria in correlation with time of incubation. The highest rate of bacterial isolate was 8,19log10, 8,26log10 after 96 and 144 hours in the sixth group, while the highest number of bacterial isolates was 7.04log10 and 6.31log10 in the third and fourth groups after 48 and 96 hours of injection respectively. The results of the statistical analysis showed a significant difference in the number of bacterial isolate after 24 hours of injection in both concentrations compared to other incubation times. A significantrelationship was also found between the amount of the dose used and the bacterial disease. this study concluded that Salmonella enterica serovar typhimurium isolated from starlings can cause pathological changes and effect on hatchery percentage in embryonated chicken eggs.
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