Iraqi Journal of Veterinary Sciences

Abstract

The aim of this study was to determine the ability of Grapes seeds (Vitis vinifera) aqueous, proteinous, non- proteinous and alcoholic extracts as antioxidant and antiatherogenic in rabbits after exposure to 0.5% H₂O₂ in drinking water for 30 days. The results illustrated capability of 0.5% H₂O₂ to initiate oxidative stress via significant decrease in Glutathione (GSH), glutathione peroxdiase (GPx), Catalase (CAT) and superoxide dismutase (SOD) level in heart and liver concomitant with significant increase in malondialhyde (MDA) in addition to decrease in high density lipoprotein- cholesterol (HDL-c) and increase low density lipoprotein- cholesterol (LDL-c) in serum of treated only with H₂O₂ rabbits. While rabbits treated with H₂O₂ associated with Grapes seeds (aqueous, proteinous, non- proteinous and alcohol) extracts for 15 days showed a significant increase of Glutathione (GSH), glutathione peroxdiase (GPx), superoxide dismutase (SOD) concentration in the heart and liver tissue as well as increase HDL-c, decrease in LDL-c and VLDL-c. While, the histolopathological examination of heart and liver tissue demonstrated that H₂O₂ induces, degeneration, necrosis and few inflammation foci in the liver and myocardial muscle. While the administration of (Vitis vinifera) aqueous, proteinous, non-proteinous and alcoholic extracts cause improvement in the histological picture of the liver and the myocardial muscle. It was concluded that the (Grapes extracts) aqueous, proteinous, non-proteinous and especially the alcoholic extracts can reduce the damage caused by H₂O₂ and this will pave the way to investigate the protective effects of the natural substances in the diseases caused by oxidative stress.