TY - JOUR ID - 167932 TI - Morphological and molecular identification of Parabronema skrjabini of camels (Camelus dromedary) in Najaf province JO - Iraqi Journal of Veterinary Sciences JA - IJVS LA - en SN - 1607-3894 AU - A-Fatlawy, Haider H. AU - Al-Fatlawi, Monyer A. AD - Department of Veterinary Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq AD - Department of Microbiology, College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah, Iraq Y1 - 2021 PY - 2021 VL - 35 IS - 3 SP - 507 EP - 512 KW - Camelus dromedary KW - Parabronema skrjabini KW - Najaf KW - Iraq DO - 10.33899/ijvs.2020.127101.1459 N2 - The current study was conducted during the period from September 2019 to December 2019, the number of examined samples 150 abomasums isolated from males 127 and females 23 to identify the species of Parabronema skrjabini that infected one-humped camel slaughtered in Al-Ashraf Najaf slaughterhouse. The microscopic examination of the worms was properties compared to other worms of the digestive system, and then confirmed using genetic markers with the polymerase chain reaction (PCR). Finally, the use of sequencing and phylogenetic analysis technologies relative to those that are predominant in world regions registered in the Gene bank. The results of the microscopic examination showed that Parabronema skrjabini distinguished by a red color, females are curved dorsally and longer than males with a vertically curved and head features that resemble a horseshoe for both sexes. The number of infected samples is 53 (35.33%) and the non-infected samples 97 (64.64%). The highest rate of infection during December month 63.41%. On the other hand, the prevalence rate has been reported 65.21% females and 29.92% males with significant differences. In this research, PCR technique was used the molecular examination with the selection of the highest DNA samples, which were 10 samples to determine the alignment range according to the ITS2 gene, all samples were well suited to primer in length 783 bp and confirmed the diagnosis of these nematodes. UR - https://www.vetmedmosul.com/article_167932.html L1 - https://www.vetmedmosul.com/article_167932_b7b1540350f99225f01630b2d08cbdf6.pdf ER -