TY - JOUR ID - 166834 TI - Bronchodilator activity of ethyl acetate extract of Nigella sativa JO - Iraqi Journal of Veterinary Sciences JA - IJVS LA - en SN - 1607-3894 AU - Mohammed Ali, Ihsan Husain AU - Abdullah, Qasim Hasso AU - AL-Habib, Omer AD - Department of Physiology and Pharmacology, College of Medicine, University of Duhok, Duhok, Iraq AD - Dean, College of Medicine, University of Duhok, Duhok, Iraq AD - College of Science, Nawroz University, Duhok, Iraq Y1 - 2021 PY - 2021 VL - 35 IS - 1 SP - 145 EP - 149 KW - N. sativa KW - Ethyl acetate extract KW - Bronchodilation DO - 10.33899/ijvs.2020.126455.1333 N2 - This study aims to investigate the mechanism(s) included in the bronchodilation effect exerted by Nigella sativa. Ethyl acetate extract (NS.EA) was prepared using a maceration method. Adult albino rats were recruited for thoracotomy and removal of the trachea. After cutting into pieces, the tissue was set in organ bath. The influence of cumulative concentrations of ethyl acetate extract was examined on contractile responses of isolated trachea to acetylcholine using different blockers such as Nifedipine (Ca2+channel blocker), Tetraethylammonium (Ca2+-activated K+ channel blocker), 4-aminopyridine (voltage-dependent K+ channel blocker), Glibenclamide (ATP-sensitive K+ channel blocker), BaCl2 (inward rectifier K+ channel blocker), methylene blue (soluble gaunylate cyclase inhibitor) and indomethacin (non-selective cyclooxygenase inhibitor). Significant inhibition of bronchodilation was observed when tracheal rings were pretreated with indomethacin and BaCl2 with (P<0.001), and with methylene blue and nifedipine with (P<0.05). The IC50s were (5.635, 6.9, 7.86 and 4.987 mg/ml) respectively. Conversely, 4-AP, GLIB and TEA showed no significant changes in the bronchodilation induced by the extract. Therefore, The Emax value for indomethacin significantly reduced from 101.34 to 73.28%, BaCl2 from 53.62 to 30.31%, methylene blue from 55.78 to 38.94% and nifedipine from 101.34 to 80.88%. On the other hand, the Emax for 4-AP and GLIB were non-significantly reduced from 53.62 to 40.14 and 40.13% respectively; and TEA more or less unchanged to 54.34%. In general, ethyl acetate extract of N. sativa induces bronchodilation through four mechanisms (activation of Kir channel, non-selective cyclooxygenase and to lesser extent the soluble guanylate cyclase, and blockade of Ca2+ channel). UR - https://www.vetmedmosul.com/article_166834.html L1 - https://www.vetmedmosul.com/article_166834_9fe125bed6c2d2056f26c8d5fc8db2f4.pdf ER -