Main Subjects : Tumor Pathology


Chemopreventive effect of Quercus infectoria galls on DMBA induced mouse skin tumorigenesis

Sherzad Ibrahim Mustafa; Bushra M. Mohammed

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2019.126267.1281

Chemoprevention is an important strategy to control the process of carcinogenesis and a number of plants with anti-cancer properties are being researched, some of which have shown promising results. Quercus infectoria galls is a well-known medicinal plant which has been used in medicine as larvicidal, anti-inflammatory, antibacterial, anti-fungal, antioxidant, and antidiabetic properties. The present study was carried out to evaluate the anti-tumor activity of the aqueous extract on the two stage process of skin carcinogenesis, consisting of initiation with a single topical application of a carcinogen 7, 12 - dimethylbenz (a) anthracene (DMBA) followed by a promoter (croton oil) three times in a week were employed. A significant reduction in tumor incidence, tumor burden, tumor yield, and cumulative number of papilloma was observed, along with a significant increase in average latent period in mice treated orally with 2 gm/kg of Quercus infectoria galls extract as compared to the positive control group treated with DMBA plus croton oil administered. Furthermore, Histopathological alterations in the carcinogen-treated control animals were also observed in the form of epidermal hyperplasia, keratinized pearl formation, and acanthosis in skin and tumors, whereas these were found to be reduced significantly before and after galls extract oral administration. The results thus concluded that Quercus infectoria galls extract exhibits significant anti-tumor activity and may serve in future drug development programs for the cancer prevention of skin cancer.

Incidence of cutaneous and subcutaneous tumors of dogs from Baghdad city: Clinical, cytological and histopathological features

Inam J. Lafta; Huda H. Alabbody

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 129-137
DOI: 10.33899/ijvs.2019.125624.1111

The present study was conducted to estimate the incidence, clinical findings, cytological and histopathological characteristics of spontaneously occurring skin neoplasms in dogs. A total of 40 grossly suspected cases of cutaneous and subcutaneous tumors were gathered during the period from July 2016 to August 2018 from male and female dogs in Baghdad city. Dogs with skin neoplasia revealed various clinical signs, and their ages were older than 5 years to 15 years. German shepherd 30% followed by Terrier dogs 25% were more influenced than other breeds. Concerning tumor features, the majority of neoplasms had solitary lesion 70%, regular shapes 65% with black color 55%. The tumors frequently occurred on fore-limbs and abdomen, and 80% of them lasted for 1-6 months. Cytological examination was valuable for initial evaluation of benign and malignant tumors. Its results were confirmed by the histopathology, in which the majority of neoplasms sized more than 5 cm and grade I. Nine different types of skin neoplasms were recognized, while 65% of them were malignant, the others were benign. Squamous cell carcinoma and adenocarcinomas were the most common comprising 35% and 25%, respectively.

Mixed thymic tumor in a rooster: gross and histological features

Saevan Saad Al-Mahmood; Ahmed Al-Qadhi; Radhwan Al-Ajeli; layth Alkattan

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 201-204
DOI: 10.33899/ijvs.2019.125536.1058

A 2-year-old rooster local breed suffered from anorexia, emaciation with abnormal large mass in lateral side in cervical region was attended to Veterinary Teaching Hospital belong to College of Veterinary Medicine, Mosul University. After physical examination a movable and friable mass were recognized in cervical region. The case sends for surgical intervention. Gross examination of removed mass showed a friable, pale, will capsulated with hemorrhagic areas. Microscopically, the mass included three types of tumors. Squamous cell thymic carcinoma characterized by presence of keratin pearl. Lymphoma that characterized by atypical polymorphic lymphocytes, vesicular nucleus, and pyknotic nucleoli, while cytoplasm appears eosinophilic to granular with ill- define borders. Lipoma which appear as irregular shape of adipocytes that lack their nucleus, with scattered infiltration of pyknotic lymphocytes. This is the first pathological report of mixed tumor of thymus gland in rooster that composed from squamous cell thymic carcinoma, lymphoma and lipoma.

Incidence rate of breast cancer, clinical and ultrasound approaches to diagnosing the same in dogs

V. Salautin; V. Gorinsky; A. Molchanov; G. Demkin; N. Pudovkin; S. Salautina

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 2, Pages 155-159
DOI: 10.33899/ijvs.2019.153843

Mammary gland tumors in dogs rank second among all newly diagnosed tumors in this species. Research goalwas to study clinical/morphological characteristics of breast cancer in dogs with the use of ultrasound investigation. 34 dogs of different breeds and age groups with spontaneously emerging neoplasms of mammary glands were chosen for the study. To classify malignant tumors, the histological classification was applied. Complete examination of breast tumors was carried out with the help of clinical observation, ultrasound, radiologic, hematological and ultrasonographic methods applied. The largest percentage (23.5%) of animals with mammary gland neoplasms are mongrel female dogs. The breed predisposition was detected in dogs of the following breeds: Miniature Schnauzer - 11.9%, Standard Dachshund - 11.9%, American Bulldog, American Cocker Spaniel and Miniature Poodle - 8% each. Breast cancer was diagnosed in 29 animals (85.3%), 100% of those cases in the nodular form with microcalcifications in the tumor parenchyma. The horizontal orientation of growth is more often typical of breast cancer in the mammary gland pairs I and II at the initial stage of carcinogenesis, and is characterized by an inhomogeneous hypoechoic structure with sufficiently pronounced boundaries detected during the ultrasound investigation. The vertical orientation of growth is mainly typical of breast cancer in the mammary gland pairs III, IV and V from the very beginning of the disease. The ultrasonography usually shows: inhomogeneous hypoechoic structure containing inclusions in the form of cords or foci of a higher acoustic solidity; also clearly defined lateral acoustic shadows and dorsal echo enhancement; as well as unevenness and indistinctness of borders. The performed histological study within the current research revealed a multifocal, infiltrating epithelial neoplasm with distinct edges, surrounded by a large amount of fibrous tissue. The tumor consisted of a number of acini with a tendency of forming a network structure. The shape of cells was detected from round to irregular, the cytoplasm was found scarce, often with uneven borders, with the large central core, with chromophilic substance and 1-2 nucleoli. The cells were characterized by the pronounced anisocytosis and anisocariosis, and a great number of mitotic figures. Lymphatic invasion was observed. Ultrasound investigation of the mammary gland allows for performing percutaneous minimally invasive interventions and differential diagnostics of cystic and solid lesions of the mammary gland (96-100% accuracy), mastopathy and carcinoma conditions.

Vulvar and vaginal tumors in Hawshar dogs

B.T. Abbas; S.M.A. Hassan; A.K. Saeed; D.M. Amin

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 67-72
DOI: 10.33899/ijvs.2018.153800

Twelve Hawshar bitches were (3-5 years in aged) examined clinically and vulvovaginal tumor masses were diagnosed histopathologically revealed the three types of tumor which include; (three cases of vulvovaginal diffuse small cell lymphoma, six cases of vaginal transmissible venereal tumor, and three cases of leiomyoma), these masses were removed surgically with local excision with or without episiotomy and recurrence of the tumor showed in some cases after following up for different duration. In conclusion, this study revealed that the age with physical condition of the dog were played an important role in cancer occurrence, and the surgical excision was not regarded as the ideal treatment especially in malignant tumor.