Main Subjects : Large Ruminant

Ultrasonography of left displaced abomasum in local cattle breed

Osama M. Aliraqi

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126447.1328

The aims of the current study were a definitive diagnosis of left displaced abomasum by using ultrasonography furthermore determination of abomasal position and measurements. Study includes sixteen local cattle breed, six have anorexia, weakness and drop milk production, on clinical examination there was decrease rumen contractions 3.6 \ 5 minutes, furthermore a pinging sound heard on auscultation of left intercostal spaces there were diagnosed as left displaced abomasum, other ten clinically healthy cows considered as normal control group. Ultrasonographic imaging was performed for stand nonsedated cows by use 3.5-5 MHz transducer at the ventral midline and in the intercostal spaces 9, 10, 11, and 12 from the left side. Ultrasonography revealed a left side displaced abomasum to an area between the abdominal wall and the rumen, which appeared as hypoechoic fluid content ventrally and hyperechoic parallel echogenic lines of gas gap dorsally, abomasal folds appear as filiform echogenic lines. While in healthy cows an abomasum located in the ventral midline and directed mainly to the right side. In conclusion, ultrasonography as a non-invasive technique could be used as a confirmative diagnosis of left displaced abomasal and help for differential diagnosis of left abomasum displacement a well as can be useful for measurement of abomasum in healthy local breed cattle.

Molecular characterization of heat shock protein 70 gene in Iraqi buffalo

Hassan N. Habib

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 139-143
DOI: 10.33899/ijvs.2019.125633.1116

The heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the hsp 70 gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism of the hsp 70 gene in Iraqi buffaloes and study bits effects on the resistance to stress. This study was conducted during from November 2018 to February 2019. The number of buffalo females used was 35 at the age of 4 - 6 years, which belonged to the local farmers from Basra city, Iraq. The DNA was extraction from the blood samples then the polymerase chain reaction (PCR) amplification was performed. The DNA sequences were analyzed by using bioinformatics analysis. The results of the molecular analysis showed that there were two groups of the hsp 70 gene as a compare with the same genes in GenBank due to silent and missense mutations. Based on these findings, it can be concluded that the Iraqi buffaloes have adapted to the surrounding environmental conditions as a result of the action of HSP 70 proliferation. The hsp 70 gene was a useful biomarker of stress tolerance in buffaloes.