Main Subjects : Veterinary Internal Medicine

Ultrasonography of left displaced abomasum in local cattle breed

Osama M. Aliraqi

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126447.1328

The aims of the current study were a definitive diagnosis of left displaced abomasum by using ultrasonography furthermore determination of abomasal position and measurements. Study includes sixteen local cattle breed, six have anorexia, weakness and drop milk production, on clinical examination there was decrease rumen contractions 3.6 \ 5 minutes, furthermore a pinging sound heard on auscultation of left intercostal spaces there were diagnosed as left displaced abomasum, other ten clinically healthy cows considered as normal control group. Ultrasonographic imaging was performed for stand nonsedated cows by use 3.5-5 MHz transducer at the ventral midline and in the intercostal spaces 9, 10, 11, and 12 from the left side. Ultrasonography revealed a left side displaced abomasum to an area between the abdominal wall and the rumen, which appeared as hypoechoic fluid content ventrally and hyperechoic parallel echogenic lines of gas gap dorsally, abomasal folds appear as filiform echogenic lines. While in healthy cows an abomasum located in the ventral midline and directed mainly to the right side. In conclusion, ultrasonography as a non-invasive technique could be used as a confirmative diagnosis of left displaced abomasal and help for differential diagnosis of left abomasum displacement a well as can be useful for measurement of abomasum in healthy local breed cattle.

Clinical, serological and antigenic study of feline panleukopenia virus in cats in Baghdad, Iraq

Mohammad M. Zenad; Amanee M. Radhy

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 435-439
DOI: 10.33899/ijvs.2019.125960.1201

 (FPL) is a common contagious disease with high morbidity and mortality rates. This study was performed in the Baghdad capital city of Iraq from January 2018 -to- February 2019. Fecal and blood samples were collected from both diarrheic and non-diarrheic 180 cats, of both sexes. Hundred pet and eighty stray cats were divided into 2 groups according to their ages: > 1-year and < 1-year. Fecal samples were checked for presence of FPL virus antigens by rapid antigen test kit (immunochromatography assay) and blood samples were tested for presence of FPL virus specific antibodies by ELISA test as well as the study of blood parameters of cats. Forty cats 22.2% were infected with FPL virus by ICG assay, while a high percentage of total seropositive rate 65 (36.1%) was founded by ELISA test. Significant higher infection 27.5% and seropositive 36.7% rates were observed in cats less than one-year age. Clinically the infected cats showed multi-systemic signs and the vomiting was the more frequent sign 87.5%, hematological changes showed significant decrease in hemogram values and prolonged clotting time, the total leukocytic count was lowered in infected cats and this owing to significant decrease in absolute numbers of lymphocytes and neutrophils. In conclusion FPL virus was widely spread in Baghdad and higher infection rate was recorded in a stray cat.

Molecular characterization of heat shock protein 70 gene in Iraqi buffalo

Hassan N. Habib

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 139-143
DOI: 10.33899/ijvs.2019.125633.1116

The heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the hsp 70 gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism of the hsp 70 gene in Iraqi buffaloes and study bits effects on the resistance to stress. This study was conducted during from November 2018 to February 2019. The number of buffalo females used was 35 at the age of 4 - 6 years, which belonged to the local farmers from Basra city, Iraq. The DNA was extraction from the blood samples then the polymerase chain reaction (PCR) amplification was performed. The DNA sequences were analyzed by using bioinformatics analysis. The results of the molecular analysis showed that there were two groups of the hsp 70 gene as a compare with the same genes in GenBank due to silent and missense mutations. Based on these findings, it can be concluded that the Iraqi buffaloes have adapted to the surrounding environmental conditions as a result of the action of HSP 70 proliferation. The hsp 70 gene was a useful biomarker of stress tolerance in buffaloes.

Prevalence and some risk factors of bovine heamotropic mycoplasma in Nineveh province - Iraq

Salam Abd Esmaeel; Basima albadrani

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 427-431
DOI: 10.33899/ijvs.2019.163170

The objective of current study was to determine the prevalence of bovine heamotropic mycoplasma in cattle using conventional polymerase chain reaction (PCR) in Nineveh province/ Iraq, to investigate some of the epidemiological risk factors associated with occurrence of the disease. From September 2017 - September 2018, four hundred blood samples were obtained from cattle both sexes, different ages, origin, different management systems and from different regions in Nineveh province, ten milliliter blood were collected from the jugular vein for PCR test, whatever epidemiological data were collected through interview with the farms owners. The results of this study appear that the prevalence of bovine heamotropic mycoplasma was 75%. The risk factors associated with increased prevalence of disease include age, increased prevalence was at >3 years, females, imported animals, indoor animals which were 86.8, 80, 91.7, 77.5% respectively. The significantly increased prevalence of the disease 93.7% in the western regions of Nineveh province. In spring and summer months a significantly increased prevalence of disease which were 79.6 and 78.95 respectively. In conclusions, this study detected that bovine heamotropic mycoplasma was widely distributed in Nineveh province associated with several risk factors.

Possibilities of urolithiasis crystallodiagnostics

A.K. Martusevich; L.M. Kozlova

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 1, Pages 23-27
DOI: 10.33899/ijvs.2017.126706

Nowadays, one of the most common groups of diseases in veterinary medicine is the urinary system pathology. Urolithiasis is widespread disease, could be found in many species, including cats, dogs, rabbits, guinea-pigs, turtles etc. Despite the large scale of this pathology in animal world, there are some challenges with diagnostic process and diagnosis’s verification. The aim of our study is estimation of diagnostic value of advanced urine tesiocrystalloscopy in urolithiasis (by the example of cats). We studied crystallogenic and initiated properties of 24 healthy cats and 32 animals with urolithiasis. Own and initiated crystallogenesis of the urine specimens was studied. For teziographic test we used sodium chloride solution (0.45%, 0.9%, 3% consequently), hydrochloric acid solution (0.1H) and sodium hydroxide (0.1H) as a crystal-forming substances. We used the original criterions to estimate crystalloscopic and tezigraphic facias. As the main parameters structure index (SI), crystallizability (Cr), facia's destruction degree (FDD) and edge belt intensity (EB) were used to describe free crystallogenesis, and main tezigraphic coefficient (Q), belt coefficient (B) and FDD were used for the comparative tezigraphy data. Results showed that Cat’s urine in normal conditions has moderate crystallogenic activity, but in urolithiasis it acquires high level of crystallizing, with intermedium value of structure index, and significant destruction of crystal-forming elements. A similar changes of physical-chemical biomedium properties are detected during analysis of tezigraphic microslides of urines of cats with urolithiasis, that was prepared using 0.9% sodium chloride as basis substance. In conclusion, we fixed that tesiocristalloscopic „pattern“ of cats’ urine in urolithiasis significantly transforms into activation of crystal formation and increasing of biomedium’s initiating potential. So, the investigation of free or initialized urine crystallization in urolithiasis has diagnostic value.

Changes in blood gases and electrolytes in local calves affected with diarrhea

Israa A. Al-Robaiee; Maab I. Al-Farwachi

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 41-45
DOI: 10.33899/ijvs.2012.166411

 The aim of this study was to investigate the changes in the blood pH, blood gases concentration , electrolytes and changes in some haematological parameters and total protein in Iraqi local breed calves affected with diarrhea from both sexes, aged between 1– 30 days old during the period from November 2010 to March 2011. The study was conducted on 40 blood samples (30 calves affected with diarrhea and ten clinically normal animals served as a control group). Results of the hematological analysis revealed significant increased in the means of hemoglobin concentration, packed cell volume and total protein concentration in those affected with diarrhea in comparison with the mean of the control animals. The results also indicated a significant decreased in the means of the blood pH, Partial pressure of oxygen concentration base excess and the sodium and bicarbonate ions concentrations with a significant increased in the mean of anion gap in calves affected with diarrhea in comparison with the means of the control animals. After calculation of the bicarbonate ion: carbonic acid ratio the results showed significantly decreased in this ratio in the calves affected with diarrhea in comparison with the values in the control animals. On the basis of this result, it have been concluded that there are three types of acidosis including uncompensated metabolic acidosis, mixed acidosis (respiratory and metabolic) and compensate metabolic acidosis.

Comparison between ELISA and other serological tests for diagnosis of brucellosis in sheep in Ninevah Province

O.KH. Al-Hankawe; M.S. Rhaymah

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 97-103
DOI: 10.33899/ijvs.2020.166880

The study was conducted to compare between the ELISA test and other serological tests (Rose bengal, modified rose bengal, tube agglutination and 2-mercapto-ethanol tests), for diagnosis of Brucellosis in sheep, and to identify the prevalence of the disease. The study included 5723 heads representing 48 flocks, 364 sera were collected from adults of both sexes from different seven geographical areas in NinevahProvince. The total prevalence of abortion was (%2.4) this value varied according to different areas. The highest in Telkef and Mosul center was (3.5%) and the lowest in Bahsheka and Shamseat was (1.9%). using the Rose-bengal test as a screening test to identify the prevalence of the disease, the total prevalence was (%11.8) and this value varied according to the areas, the highest prevalence was in Al-Shekan (22.7%), then Telkef (18.5%), while the lowest was in Mosul city center (6%). The study revealed that the prevalence of the disease varied according to the type of the serological tests were used. ELISA recorded the highest (15.9%) then the modified Rose-bengal test (13.4%), Rose-bengal test (11.8%), tube agglutination test (6.9%) and 2-Mercapto-ethanol test recorded (8.2%). When comparing the positive results of ELISA and other serological tests (Rose-Bengal, Modified rose-bengal, Tube agglutination, 2-mercapto-ethanol tests) the degree of agreement was (74.1%), (84.5%), (43.1%) and (51.7%) respectively. The results reveals significant (P<0.05) difference between ELISA and other serological tests.