Main Subjects : Veterinary Internal Medicine

Detection of canine distemper virus in stray and pet dogs in Mosul city, Iraq

Muhanad M. Saaed; Qaes T. Alsarhan

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 2, Pages 315-319
DOI: 10.33899/ijvs.2021.130127.1739

The current work was carried out during the period from September, 2020 to March, 2021 in Mosul city, Iraq, with the objectives to detection of canine distemper virus (CDV) for the first time in Mosul using microscopic examination of blood smears, rapid serum antigen test and sandwich ELISA test as well as determine the agreement between the different diagnostic methods. A total of 92 blood samples were collected from suspected dogs with CDV (69 stray dogs and 23 Pet dogs). A primarily detection of CDV in blood smear by the presence of inclusion bodies in erythrocytes and leukocytes, followed by detection of CDV antigen in serum using rapid test and sandwich ELISA test. Results indicate that the infection rates of CDV in dogs were 32.6%, 13%, and 19.5% using microscopic examination, rapid test and sandwich ELISA test respectively. A statistically significant higher infection rate was reported in stray dogs compared to pet dogs based on all diagnostic tests used in this study. Based on Kappa values 0.413, 0.675, 0.745, there were moderate agreement between microscopic examination and rapid test, and substantial agreement between microscopic examination and sandwich ELISA test, also between rapid test and sandwich ELISA test respectively. In conclusions, CDV is widespread in dogs in Mosul city, Iraq and all tests used in this study are efficient for detection CDV based on compatibility between them.

Ultrasound image analysis for detection of fatty liver and grading its severity in buffaloes at Mosul, Iraq

Ziena H. Hasan; Osamah M. aliraqi; Ielaf O. Abdul Majjed

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 2, Pages 507-511
DOI: 10.33899/ijvs.2021.130666.1864

The study aimed to (ⅰ) examines whether quantitative and qualitative digital analysis of hepatic ultrasound images can be used to detect fatty liver and grading its severity, (ⅱ) to estimate the concentration of serum β-hydroxybutyrate content in normal and diseased animals. A total of 50 buffaloes (15 clinically normal and 35 suffered from decreased milk production, loss of appetite, and loss of body weight) 2-4 weeks after parturition were studied. Buffaloes were examined by real-time ultrasound using a 3.5 MHz convex probe. Ultrasound images were saved for qualitative and quantitative analysis, using computer aid Fatty-Liver software (programmed in Matlab language). Based on the digital analysis of ultrasound images, the diseased buffaloes were classified as mild (n=20), moderate (n=7) and severe (n=8) fatty liver affection. Results indicated that the concentration of β-hydroxybutyrate estimated via ELISA were 0.409, 0.735, 0.923, 1.708 mmol/dL in normal, mild, moderate, and severe diseased buffaloes respectively. However, a significant difference has been encountered among normal and diseased animals. It has been concluded that buffaloes at Mosul suffered from fatty liver and digital analysis of ultrasound images was a suitable method for clinical diagnosis, furthermore, estimation of β-hydroxybutyrate was a suitable method for laboratory diagnosis.

Subclinical ketosis: Prevalence and some risk factors in cross breed and imported breed dairy cows in Mosul, Iraq

Mohsin H. Ali; Sadam D. Hassan

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 2, Pages 273-277
DOI: 10.33899/ijvs.2021.129949.1707

Although ongoing control strategies are attempting to minimize certain metabolic disorders such as hyperketonemia, factors such as genetics, management, environment, and geography still present a potential challenge to the achievement of the desired results. In light of this, this study was conducted (i) to determine the prevalence of subclinical and clinical ketosis based on the concentration of ketone bodies (BHB) using ELISA Kit, and (ii) to examine a number of epidemiological risk factors related to the conditions. From October, 2020 to the January, 2021. A 80 blood samples were collected in a cross-sectional survey in different areas of Mosul. Data included age, management systems, parity, and origin. At the cut-off point ≥1.2-≤ 1.4 mmol / L, the prevalence of subclinical ketosis was 27.5% and for clinical ketosis it was 5%. Results revealed that the animals aged > 3 years were more at risk for subclinical ketosis compared to animals aged ≤ 3 years RR = 2.6471. Multiparous animals were more at risk compared to the primiparous (two or less births) RR = 2.0652. No significant difference between the animals’ origins RR = 0.6863. Indoor and outdoor system animals were more at risk for subclinical ketosis compared to the indoor system only RR = 2.1389. In conclusion, hyperketonemia is prevalent in Mosul. Certain risk factors should be taking into account when planning control programs. Attention should be paid to diagnosis, management, and control of this disease during the risk stage to avert economic loss to the dairy farmers.

Orf disease in local goat; clinical and phylogenetic study in Al-Qadisiyah governorate, Iraq

Khalefa A. Mansour; Muthanna H. Hussain; Asaad J. Abid; Qassim H. Kshash

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 1, Pages 117-121
DOI: 10.33899/ijvs.2021.129489.1651

This study aimed to diagnose orf virus ORFV from infected goats in Al-Qadisiyah Governorate of Iraq, using polymerase chain reaction and construction of phylogenetic tree. Clinical examination was made on 400 goats in different herds and crusty scabs were collected from forty infected goats. All scabs were subjected to (PCR), sequencing, and phylogenetic analysis involving the presence of GIF gene GM-CSF* inhibitor factor, a characteristic gene of the ORFV. The typical proliferative skin lesion with overall orf prevalence results by PCR was 10% with low mortality rates and high significance variation with age and gender. Two positive PCR amplicons were sent for partial-gene-based sequencing and Phylogenetic analysis. Multiple alignments of sequences presented close identities with two Iraqi global isolates of the virus. This study didn't only report novel sequences from the local orf virus isolates, but it also provides important data about transmission of local sheep orf virus into local goat during closely rearing and grazing and orf outbreak in small ruminant in Iraq might be suspected. 

Clinical and subclinical mastitis in buffalue in Mosul area, Iraq

Abdulsatar S. Sadoon

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 1, Pages 177-186
DOI: 10.33899/ijvs.2021.129644.1671

This paper aims to investigate the occurrence of clinical and sub-clinical cases of mastitis in buffaloes, and to identify the differences in the components of the mastitis milk, to facilitate the investigation of a number of epidemiological risk aspects in relation to the occurrence of mastitis and to detect the different bacterial species associated with the disease. Eight hundred buffalo milk samples were randomly extracted from the animals of different ages in Mosul city, Iraq and examined by California mastitis test (CMT) and Ultrasonic milk analyzer. According to the clinical signs and CMT, the results indicated the prevalence of the clinical and subclinical cases of the disease in buffaloes to be 10.62% and 27.37% respectively. There was a substantial rise in the population of somatic cells /ml in both clinical and subclinical cases of mastitis. The risk factors included third trimester of lactation period, old ages 10-11 years, outdoor feeding animals, > 30 buffaloes/herd, northern and southern parts of the city, Spring and Winter seasons. In clinical instances of mastitis, the occurrence of Staphylococcus aureus was 25.88%, Streptococcus agalactiae 18.82%, Streptococcus pyogenes 16.47%, Corynebacterium bovis 14.11%, Escherichia coli 10.58%, Pseudomonus aeruginosa 7.05%, and Pasteurella multocida 7.05%. In the subclinical cases of mastitis, the prevalence of Staphylococcus chromogens was 14.61%, Staphylococcus xylosus 12.78%, Streptococcus agalactiae 11.87%, Streptococcus dysagalactiae 11.41%, Streptococcus uberis 10.04%, Proteus vulgaris 10.04%, Klebsiella pneumonae 9.58%, Escherichia coli 8.21%, Corynebacterium bovis 7.30%, and Pasteurella multocida 4.10%. It is concluded that mastitis leads to a significant reduction in the levels of total solids, lactose, protein, fat, density, and a significant rise in the levels of pH values and electrical conductivity of the milk samples of clinical and subclinical cases of mastitis.

The effectiveness of biogenic silver nanoparticles in the treatment of caprine mastitis induced by Staphylococcus aureus

Ali H. Al Dujaily; Alaa K. Mahmood

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue Supplement I-III, Pages 73-78
DOI: 10.33899/ijvs.2021.131415.1946

The aim of the present study is to determine the efficiency of silver nanoparticles in mastitis treatment induced by Staphylococcus aureus, 20 lactating goats were infected intramammaryinoculated with 1.5×108 cfu/ml of virulence Staphylococcus aureus isolated from milk of caprine mastitis and at 48hr post inoculation, the animals were divided randomly into four groups equally, 1st group left without treatment as control positive, 2nd group treated intramammaryinfusion with 5 ml Silver nanoparticle after 2h from infection daily for 3 consecutive days, 3rd group treated intramammaryinfusion with 5 ml Silver nanoparticle after 72h from infection daily for 3 consecutive days, 4th group treated intramammaryinfusion with 5 ml Silver nanoparticle mixed with gentamycin after 72h from infection daily for 3 consecutive days. The silver nanoparticles used in this study were created using a green method from Bacillus clausii. The Scanning electron microscope was spherical and homogeneous form, with a size range of 25 to 45 nm. Results showed severe clinical signs of the mammary gland with heavy bacterial isolation in 1st control groups at 3, 6-, 9-, 14- and 21-days post infection, while animals that were intramammary treated with Silver nanoparticle alone or combination with gentamycin showed a disappearance in clinical signs of the mammary gland and bacterial isolation, it was concluded that Silver nanoparticle had a strong action against mastitis caused by Staphylococcus aureus.

Seroprevalence of Crimean Congo Hemorrhagic Fever in cows by ELISA in Mosul city

Salam A. Esmaeel; Khder J. Hussain; Mohammad A. Al-Taliby

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 4, Pages 803-807
DOI: 10.33899/ijvs.2021.128668.1595

Crimean-Congo hemorrhagic fever (CCHF) is described as a tick-borne viral zoonosis highly prevalent in Africa, Asia, Russia and the Balkans within the distribution range of ticks that belong to the genus Hyalomma. This research aimed to verify the seroprevalence of CCHF in cows employing Indirect enzyme-linked immunosorbent assay (I-ELISA) in Mosul city / Iraq, to ​​examine some epidemiological risk factors related to the incidence of CCHF. From October 2019 - September 2020, one hundred eighty-four blood samples were taken from 3-8-year-old cattle of both sexes, from several management systems and origins, from various parts in Mosul city, 10 ml of blood was taken from the jugular vein in test tubes without anticoagulant to obtain the sera for I-ELISA test, while epidemiological data were obtained by interviewing the farm-owners. I-ELISA was applied to detect the antibodies of CCHF in the serum. The overall seroprevalence of CCHF in cows was 40/184 (21.7%). As for the risk factors associated with increased seroprevalence of the disease, an increase in seroprevalence rates was observed at ages 6, 7 and 8 years 30.5%, among females 26.3% and imported animals 27.5%. which showed that the serological prevalence in indoor feeding and outdoor feeding, was not significantly different (p<0.05). This study concluded that there is a higher seroprevalence of CCHF in Mosul city along with many risk factors related to its incidence.

Ultrasonography of left displaced abomasum in local cattle breed

Osama M. Aliraqi

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 1, Pages 139-144
DOI: 10.33899/ijvs.2020.126447.1328

The aims of the current study were a definitive diagnosis of left displaced abomasum by using ultrasonography furthermore determination of abomasal position and measurements. Study includes sixteen local cattle breed, six have anorexia, weakness and drop milk production, on clinical examination there was decrease rumen contractions 3.6 \ 5 minutes, furthermore a pinging sound heard on auscultation of left intercostal spaces there were diagnosed as left displaced abomasum, other ten clinically healthy cows considered as normal control group. Ultrasonographic imaging was performed for stand nonsedated cows by use 3.5-5 MHz transducer at the ventral midline and in the intercostal spaces 9, 10, 11, and 12 from the left side. Ultrasonography revealed a left side displaced abomasum to an area between the abdominal wall and the rumen, which appeared as hypoechoic fluid content ventrally and hyperechoic parallel echogenic lines of gas gap dorsally, abomasal folds appear as filiform echogenic lines. While in healthy cows an abomasum located in the ventral midline and directed mainly to the right side. In conclusion, ultrasonography as a non-invasive technique could be used as a confirmative diagnosis of left displaced abomasal and help for differential diagnosis of left abomasum displacement a well as can be useful for measurement of abomasum in healthy local breed cattle.

Clinical, serological and antigenic study of feline panleukopenia virus in cats in Baghdad, Iraq

Mohammad M. Zenad; Amanee M. Radhy

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 435-439
DOI: 10.33899/ijvs.2019.125960.1201

 (FPL) is a common contagious disease with high morbidity and mortality rates. This study was performed in the Baghdad capital city of Iraq from January 2018 -to- February 2019. Fecal and blood samples were collected from both diarrheic and non-diarrheic 180 cats, of both sexes. Hundred pet and eighty stray cats were divided into 2 groups according to their ages: > 1-year and < 1-year. Fecal samples were checked for presence of FPL virus antigens by rapid antigen test kit (immunochromatography assay) and blood samples were tested for presence of FPL virus specific antibodies by ELISA test as well as the study of blood parameters of cats. Forty cats 22.2% were infected with FPL virus by ICG assay, while a high percentage of total seropositive rate 65 (36.1%) was founded by ELISA test. Significant higher infection 27.5% and seropositive 36.7% rates were observed in cats less than one-year age. Clinically the infected cats showed multi-systemic signs and the vomiting was the more frequent sign 87.5%, hematological changes showed significant decrease in hemogram values and prolonged clotting time, the total leukocytic count was lowered in infected cats and this owing to significant decrease in absolute numbers of lymphocytes and neutrophils. In conclusion FPL virus was widely spread in Baghdad and higher infection rate was recorded in a stray cat.

Molecular characterization of heat shock protein 70 gene in Iraqi buffalo

Hassan N. Habib

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 139-143
DOI: 10.33899/ijvs.2019.125633.1116

The heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the hsp 70 gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism of the hsp 70 gene in Iraqi buffaloes and study bits effects on the resistance to stress. This study was conducted during from November 2018 to February 2019. The number of buffalo females used was 35 at the age of 4 - 6 years, which belonged to the local farmers from Basra city, Iraq. The DNA was extraction from the blood samples then the polymerase chain reaction (PCR) amplification was performed. The DNA sequences were analyzed by using bioinformatics analysis. The results of the molecular analysis showed that there were two groups of the hsp 70 gene as a compare with the same genes in GenBank due to silent and missense mutations. Based on these findings, it can be concluded that the Iraqi buffaloes have adapted to the surrounding environmental conditions as a result of the action of HSP 70 proliferation. The hsp 70 gene was a useful biomarker of stress tolerance in buffaloes.

Prevalence and some risk factors of bovine heamotropic mycoplasma in Nineveh province - Iraq

Salam Abd Esmaeel; Basima albadrani

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 427-431
DOI: 10.33899/ijvs.2019.163170

The objective of current study was to determine the prevalence of bovine heamotropic mycoplasma in cattle using conventional polymerase chain reaction (PCR) in Nineveh province/ Iraq, to investigate some of the epidemiological risk factors associated with occurrence of the disease. From September 2017 - September 2018, four hundred blood samples were obtained from cattle both sexes, different ages, origin, different management systems and from different regions in Nineveh province, ten milliliter blood were collected from the jugular vein for PCR test, whatever epidemiological data were collected through interview with the farms owners. The results of this study appear that the prevalence of bovine heamotropic mycoplasma was 75%. The risk factors associated with increased prevalence of disease include age, increased prevalence was at >3 years, females, imported animals, indoor animals which were 86.8, 80, 91.7, 77.5% respectively. The significantly increased prevalence of the disease 93.7% in the western regions of Nineveh province. In spring and summer months a significantly increased prevalence of disease which were 79.6 and 78.95 respectively. In conclusions, this study detected that bovine heamotropic mycoplasma was widely distributed in Nineveh province associated with several risk factors.

Possibilities of urolithiasis crystallodiagnostics

A.K. Martusevich; L.M. Kozlova

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 1, Pages 23-27
DOI: 10.33899/ijvs.2017.126706

Nowadays, one of the most common groups of diseases in veterinary medicine is the urinary system pathology. Urolithiasis is widespread disease, could be found in many species, including cats, dogs, rabbits, guinea-pigs, turtles etc. Despite the large scale of this pathology in animal world, there are some challenges with diagnostic process and diagnosis’s verification. The aim of our study is estimation of diagnostic value of advanced urine tesiocrystalloscopy in urolithiasis (by the example of cats). We studied crystallogenic and initiated properties of 24 healthy cats and 32 animals with urolithiasis. Own and initiated crystallogenesis of the urine specimens was studied. For teziographic test we used sodium chloride solution (0.45%, 0.9%, 3% consequently), hydrochloric acid solution (0.1H) and sodium hydroxide (0.1H) as a crystal-forming substances. We used the original criterions to estimate crystalloscopic and tezigraphic facias. As the main parameters structure index (SI), crystallizability (Cr), facia's destruction degree (FDD) and edge belt intensity (EB) were used to describe free crystallogenesis, and main tezigraphic coefficient (Q), belt coefficient (B) and FDD were used for the comparative tezigraphy data. Results showed that Cat’s urine in normal conditions has moderate crystallogenic activity, but in urolithiasis it acquires high level of crystallizing, with intermedium value of structure index, and significant destruction of crystal-forming elements. A similar changes of physical-chemical biomedium properties are detected during analysis of tezigraphic microslides of urines of cats with urolithiasis, that was prepared using 0.9% sodium chloride as basis substance. In conclusion, we fixed that tesiocristalloscopic „pattern“ of cats’ urine in urolithiasis significantly transforms into activation of crystal formation and increasing of biomedium’s initiating potential. So, the investigation of free or initialized urine crystallization in urolithiasis has diagnostic value.

Evaluation of role of probiotic IMBO reduce the effect of collibacilosis in newborn calves

A.J. Aletabi; J.M. Khalaf; A.S. Albana

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. IV, Pages 321-328
DOI: 10.33899/ijvs.2012.168754

The aim of this sutdy is to evaluate the effect of using the (IMBO) as probiotic in the reduction of diarrhea caused by E.coli in newborn calves. Using 150 newborn calves naturally infected with E.coli which is divided into three groups 50 animals in each group. First group, the animals of this group were inoculated Biomin IMBO as probioric orally given daily for 16 day with 6 gm\animal, while second group, the animals of this group were inoculated IMBO as probitic with same previous dose after natural infected with E.coli and suffering from diarrhea. While the third group (control group) which was not inoculated the IMBO as probiotic. The E.coli was isolated and it is virulence was evaluated by k99 pili test, the bacterial count for the feces sample of the calves was done as many hematological test which include (leukocyte count, differential WBCs count, packed cell volume, as well as TPP concentration and albumin and globulin for day (1,7,16) of the study. The probiotic was positively effective in the decrease of the severity of clinical sings and significantly increase calves weight which were treated by the probiotic, in the day (16) of the study and increase in the mortality rate compared with control group. The study shows probiotics use contributed in decrease of the number of E.coli excreted by feces of naturally infected calves with colibacillosis. Hematology test results showed significant increase in total leukocyte count, neutrophil, monocyte count and significant decrease in pact cell volume in the first and second group compared with control group. While serum test showed significant increase in total protein and globulin and significant decrease in albumin value. From the results of the study we notice the positive effect of probiotic IMBO on the health state after causing infection by colibacillosis and decrease the severity of clinical signs and increase the immunity respond.

Prevalence and pathological study of schistosomiasis in sheep in Akra/Dohuk province, northern Iraq

I.K. Zangana; K.J. Aziz

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. IV, Pages 125-130
DOI: 10.33899/ijvs.2012.168750

The occurrence of Schistosomiasis was detected by fecal examination (Formalin- ether centrifugalsedimentation technique) and abattoir Survey during a period from October 2006 to March 2007,andhistopathological study. Schistosomabovis was identified with prevalence rate 11.37% by fecal examination where as 1.6% by abattoir survey. Diseased animals exhibited acute clinical signs, which included emaciation, sunken eye, pale mucus membrane and diarrhea. The Infections was more prevalent in age 1-3 years 15.9%. The histopathological findings reveal characteristic lesions in blood vessels and some tissues including thrombosis in veinuoles and mesenteric vein and minute granuloma around the eggs in portal area and fatty necrosis in the liver, cystic structure of some mucosal glandsof the intestine containing eggs, with infiltration of mononuclear inflammatory cells in different tissues.

Changes in blood gases and electrolytes in local calves affected with diarrhea

Israa A. Al-Robaiee; Maab I. Al-Farwachi

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 41-45
DOI: 10.33899/ijvs.2012.166411

 The aim of this study was to investigate the changes in the blood pH, blood gases concentration , electrolytes and changes in some haematological parameters and total protein in Iraqi local breed calves affected with diarrhea from both sexes, aged between 1– 30 days old during the period from November 2010 to March 2011. The study was conducted on 40 blood samples (30 calves affected with diarrhea and ten clinically normal animals served as a control group). Results of the hematological analysis revealed significant increased in the means of hemoglobin concentration, packed cell volume and total protein concentration in those affected with diarrhea in comparison with the mean of the control animals. The results also indicated a significant decreased in the means of the blood pH, Partial pressure of oxygen concentration base excess and the sodium and bicarbonate ions concentrations with a significant increased in the mean of anion gap in calves affected with diarrhea in comparison with the means of the control animals. After calculation of the bicarbonate ion: carbonic acid ratio the results showed significantly decreased in this ratio in the calves affected with diarrhea in comparison with the values in the control animals. On the basis of this result, it have been concluded that there are three types of acidosis including uncompensated metabolic acidosis, mixed acidosis (respiratory and metabolic) and compensate metabolic acidosis.

Comparison between ELISA and other serological tests for diagnosis of brucellosis in sheep in Ninevah Province

O.KH. Al-Hankawe; M.S. Rhaymah

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 97-103
DOI: 10.33899/ijvs.2020.166880

The study was conducted to compare between the ELISA test and other serological tests (Rose bengal, modified rose bengal, tube agglutination and 2-mercapto-ethanol tests), for diagnosis of Brucellosis in sheep, and to identify the prevalence of the disease. The study included 5723 heads representing 48 flocks, 364 sera were collected from adults of both sexes from different seven geographical areas in NinevahProvince. The total prevalence of abortion was (%2.4) this value varied according to different areas. The highest in Telkef and Mosul center was (3.5%) and the lowest in Bahsheka and Shamseat was (1.9%). using the Rose-bengal test as a screening test to identify the prevalence of the disease, the total prevalence was (%11.8) and this value varied according to the areas, the highest prevalence was in Al-Shekan (22.7%), then Telkef (18.5%), while the lowest was in Mosul city center (6%). The study revealed that the prevalence of the disease varied according to the type of the serological tests were used. ELISA recorded the highest (15.9%) then the modified Rose-bengal test (13.4%), Rose-bengal test (11.8%), tube agglutination test (6.9%) and 2-Mercapto-ethanol test recorded (8.2%). When comparing the positive results of ELISA and other serological tests (Rose-Bengal, Modified rose-bengal, Tube agglutination, 2-mercapto-ethanol tests) the degree of agreement was (74.1%), (84.5%), (43.1%) and (51.7%) respectively. The results reveals significant (P<0.05) difference between ELISA and other serological tests.

Detection of brucellosis in sheep using PCR with other serological tests

H.A. Mohamed; A N. Saleem

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. IV, Pages 359-363
DOI: 10.33899/ijvs.2012.168760

The study aimed to investigate the brucellosis in sheep herds that showed, disease and signs of abortion in different regions of the Nineveh province. The study involved randomly collection of three thousand blood samples from 22 herds of sheep and from each blood samples, 485 (sheep 425 and rams 60) were tested for Rose-Bengal plate test (RBPT) as well as Tube agglutination test (TAT), then confirmed these diagnosis of the positive and negative samples of RBPT and TAT by using the polymerase chain reaction technique. The results showed variation in abortion rates 11.9% in herds under study depending on the history of the condition, which amounted from 1.6 - 21.1 %, while the abortion rates in herds based on the examination of a Rose-Bengal reach 5.4%. It was also noted a significant increase in the proportion of infected males (rams) brucellosis which reached 50%. The study found that the percentage of compatibility between the RBPT and TAT of the serum was 90.7%. Using two pairs of primers, the first one, Brucella OMP2 (193 base pairs) use to identify the genius of the Brucella, while the other pairs of primers use as specific (731 base pairs) to identify the strain of Br. melitensis only. Application of Brucella OMP2 primers on some selected result of positive and negative samples of RBPT and TAT by using PCR technique gives compatibility rate reach 94.4%.

Effect of cypermethrin and ivermectin against psoroptic mange in sheep

M. Kh. Shindala; B. A. Al- Badrani; O. A. Abdulla; N. A. Younis; R. K. Peters

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. III, Pages 263-267
DOI: 10.33899/ijvs.2012.168735

The therapeutic efficacy of cypermethrin and ivermectin against Psoroptes ovis mites that caused psoroptic mange in sheep was evaluated in 10 naturally infected sheep of both sex 2-5 years old. Cypermethrin solution in 1/1000 dilution at once time for treatment of first group (n=5), showed better therapeutic efficacy against natural infection with Psoroptic mange, than ivermectin which used for treatment of sheep in second group (n=5) at dose of 0.4mg/kg body weight subcutaneously for once time also, the results was evaluated dependent upon the order of severity of skin lesion during 3, 7 and 14 day post treatment. Conclusion the results of that study was showed ivermectin can be used for treatment of mild and moderate cases of Psoropteic mange of sheep, while cypermethrin was used against sever cases.