Main Subjects : Infectious and Epidemic Animal Diseases


Phylogenetic analysis of ovine herpes virus-2 (OHV-2) in malignant catarrhal fever infected cattle in AL-Qadisiyah of Iraq

Yahia Khudhair; Hayder N. Aeyyz; Muthanna H. Hussain

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 1, Pages 51-58
DOI: 10.33899/ijvs.2019.125522.1044

Ovine herpesvirus-2 is a member of the gammaherpesviruses of the herpeseviridae, which is the etiologic agent of malignant catarrhal fever (MCF), a significant fatal disease of cattle. MCF disease was diagnosed in native Iraqi cattle of AL-Qadisiyah governorate of Iraq, during the period from April 2014 and August 2016. Twenty three blood samples were collected from clinically suspected cattle. The presence of the virus in samples was ascertained based on clinical pictures, postmortem examination and molecular assays. Pansystemic involvement included respiratory, digestive, urinary, nervous systems and ocular lesions were described. A molecular analysis based on a tegument protein gene by specific semi-nested-PCR, DNA sequence and multiple alignments of all PCR products confirmed the Ovine herpesvirus-2 (OHV-2) infection, and revealed a single and double nucleotide deletion, insertion and substitutions,. Some of these mutations were non-silent, resulting in changes at the predicted amino acids level into viral tegument protein. The phylogenetic analysis showed the disease was caused by two genovariants of OHV-2 including at one cluster and were related to other sequences from others countries was analysed. MCF is sporadically occurring in cattle in Iraq, the head and eye form is more pronounced form. It has been concluded that study is provides valuable information about the genetic variation among the OHV-2 genotypes in Iraqi cattle. Based on sequence and phylogenetic analysis of tegument protein gene, this paper elucidated genetic relationship between identified Iraqi OHV-2 with other strains detected in other geographical regions. These results provide new information on the epidemiological and genetically of OHV-2 in Iraq.

Mastitis in mare: case report

M.SH. Rhaymah; Q.T. Al-Obaidi; M.A. Hamad; M.A. Altalby

Iraqi Journal of Veterinary Sciences, Volume 32, Issue 1, Pages 109-111
DOI: 10.33899/ijvs.2018.153831

Mastitis is rare in mares compared with cattle, a mare suffered from mastitis was referred to the Veterinary Teaching Hospital, College of Veterinary Medicine, University of Mosul for examination with a history of abortion before two months and gradually loss of body weight. Clinical examination revealed, a swollen left mammary gland, painful to palpate, there was mild degree of edema in the tissue surrounding the gland, fluid with clot expressed from the gland, body temperature was 37.4ºC and heart rate was 40/ minute. Fluid (mammary secretions), milk samples were collected under sterile condition and submitted to the laboratory for bacterial culture and identification test. The results of the culture and biochemical tests showed that the Staphylococcus spp was the etiological agent. This case was treated with systemic and local antibiotic therapy (Penicillin preparation).

Molecular identification of peste des petits ruminants virus in wild goat and domestic small ruminants by real-time -PCR technique in Erbil-Iraq

E.P. Candlan; F.P. Khoran; L. Hana

Iraqi Journal of Veterinary Sciences, Volume 31, Issue 1, Pages 51-54
DOI: 10.33899/ijvs.2017.126710

In July 2010 outbreak was occurred in wild goat in Barzan, Sherwin mizzen and Mergasur in Kurdistan Region- Iraq. There were over 2700 deaths (both young and adult) during the period of July 2010 to October 2011. Based on the clinical signs and post-mortem findings, the involvement of peste des petits ruminants virus (PPRV) was suspected. This was confirmed by Real Time PCR technique using TaqMan®probes for the detection of Peste des petits ruminants. The results of Real-Time PCR for the 9 sample taken from 9 Wild goat there are 6 sample positive and 3 sample negative and 76 sample from domestic ruminants (sheep and goat) 63 samples was negative for PPR. This result confirms the diagnosis domestic ruminants in the region are routinely vaccinated with an attenuated vaccine based on the ‘Nigeria/75/1’ strain of PPRV.