Main Subjects : Animal Toxicology


Evaluation of the heavy metal content in the muscle tissue of common carp (Cyprinus carpio L.) reared in groundwater in Basrah province, Iraq

Arafat Ahmed

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126491.1336

The concentration of heavy metals (Cu, Zn, Cr, Ni, Hg, Pb, and Cd) in the muscles of common carp (Cyprinus carpio) reared in groundwater in Khor Al-Zubair, Basrah province (in the south of Iraq) were assessed using X-ray fluorescence (XRF) spectroscopy. XRF is a powerful technique for element analysis in different environmental samples with many advantages compared with conventional laboratory methods. The mean concentration of the studied metals in the edible parts of the fish (Cr= 11.42, Ni= 2.75, Hg=1.53, Pb= 1.93, and Cd=4.42 mg/ kg dry weight) exceeded the recommended maximum acceptable levels proposed by the Food and Agriculture Organization (FAO)/World Health Organization (WHO), The commission of the European Communities (EC), and Food and Drug Administration (FDA). The results suggest that the tested fish muscle tissue was not safe for human consumption and that the groundwater in the Khor al-Zubair area is possibly contaminated with heavy metals, mainly owing to industrial activity.

Anticlastogenic properties of Quercus infectoria galls extract against DMBA induced genotoxicity in bone marrow cells of mice in vivo

Sherzad I Amedi; Bushra M. Mohammed

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 279-285
DOI: 10.33899/ijvs.2019.125930.1188

This study aimed to evaluate the aqueous extract of Quercus infectoria galls extract (QIGE) as anticlastogenic. The effect of QIGE was tested in mice (5 groups for each test) treated with 7, 12-dimethylbenz (a) anthracene (DMBA), the strong site-specific carcinogenic agent. In this study, the QIGE show no signs of toxicity, a single dose of DMBA (50 mg/kg) was injected intraperitoneally to Swiss albino mice caused a great increase in number of chromosomal aberrations, micronucleated polychromatic erythrocytes (MnPCEs) and reduction in the percentage of mitotic index (MI) (cytogenetic markers). Oral pre-treatment and post-treatment of QIGE for 14 days at dose 2 gm/kg b.w. daily to DMBA-treated animals greatly reduced in number of micronucleus formation, chromosomal abnormalities such as chromosomal break, chromatid breaks, ring chromosome, dicentric chromosome and fragments. Besides, mitotic index frequency increased comparing with the positive control. The data suggest that QIGE has potent anti-clastogenic effect against DMBA-induced genotoxicity in bone marrow cells of albino male mice and it may have a protective effect against the mutagenicity of the polynuclear aromatic hydrocarbons (PAH).

Toxicological and neurobehavioral effects of chlorpyrifos and deltamethrin insecticides in mice

Khaerea A. Mustafa; Banan Kh. Al-Baggou

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 189-196
DOI: 10.33899/ijvs.2019.125738.1144

The aim of the present study was to determine the acute toxicity of chlorpyrifos and deltamethrin in mice separately and to study their toxic and neurobehavioral effects. Median Lethal Doses (LD50) of chlorpyrifos and deltamethrin were determined depending on up and down method. The oral LD50 of chlorpyrifos was 193.05 mg/kg and of deltamethrin was 15.71 mg/kg in mice. The oral administration of chlorpyrifos 155 mg/kg and deltamethrin 12.56 mg/kg represent 80% of LD50 resulted in acute signs of poisoning that manifested by dyspnea, salivation and lacrimation at 100%, piloerection, straub tail, tremors, convulsions and death at 70% for chlorpyrifos and 60% for deltamethrin and writhing reflex at 20% for chlorpyrifos. Oral administration of chlorpyrifos 310 mg/kg and deltamethrin 24 mg/kg increased severity of toxicosis signs as a percentage of piloerection, straub tail, tremors, seizures and death 100%. As well as decrease the onset of tremors, convulsions and death, writhing reflex which appears at 20% for chlorpyrifos and 10% for deltamethrin. After three hours of chlorpyrifos and deltamethrin oral administration at doses represent 20% and 10% of LD50 there are significantly hypoactivation in open-field activity, significantly increased in the duration of negative geotaxis performance, significantly decreased in head pocking and swimming scores compared to control group. In conclusion we found that deltamethrin was more toxic than chlorpyrifos this is based on the LD50 value. However, the signs of toxicosis and neurobehavioral effects produced by both toxicants were not differential and could not be associated with the toxic level.

The effect of nicotine per inhalation on the diameter and epithelium thickness of the seminiferous tubules of rats

Yew S. En; Hardany Primarizky; Widjiati Widjiati; Epy Luqman

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 209-215
DOI: 10.33899/ijvs.2019.125725.1141

This study aimed to assess the level of testicular damage by observing the changes in the diameter and epithelium thickness of seminiferous tubules in rats that exposure to nicotine per inhalation. Thirty adult male rats were used and divided into five equal groups and treatment as follows for 20 days; Control group NaCl 0.9%, P1 nicotine 0.5 mg/kg, P2 nicotine 1.0 mg/kg, P3 nicotine 2.0 mg/kg and P4 nicotine 4.0 mg/kg. All groups were given treatment per inhalation for twenty days. At the end of treatment and the rats were sacrificed testes were collected for histopathological preparation. The testes were processed for routine paraffin embedding and staining and the sections were examined for histopathological changes. There results showed that nicotine administration induced varying degrees of structural damage to the seminiferous tubules, as the decreased in diamater and epithelium thickness of seminiferous tubules. The diameter and epithelium thickness of seminiferous tubules in four experimental groups reduced compared to the control group. This study proves that nicotine administration does decreases the spermatogenesis of rats by reducing the diameter and epithelium thickness of seminiferous tubules in testes. It also proves that the level of testicular damage is directly proportional to the dosage of nicotine administrated to male rats.