Main Subjects : DNA Viruses

Isolation and identification of Circovirus in pigeon

Safwan Yousif Al-Baroodi; Mozahim yasen Al-Attar

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126706.1364

The purpose of this study is first trial to detect of pigeon circovirus, so 1sr group include 100 cloacal swabs were collected 55 healthy and 45 ill pigeons, 36 yearlings and 64 adults, the 2nd group included organs was liver, spleen, bursa of Fabricius from 41 young pigeons 10-30 days old and bursa of Fabricius, liver, spleen from 28 dead in shell pigeon embryo in the 3rd group. DNA extracted from this samples and detection of virus DNA was attempt using polymerase chain reaction, after DNA amplification, the final products of the amplicon with 331 bp was cleared by using electrophoresis using agarose gel at concentration 2%. Results of viral DNA amplification were positive, which revealed as band in 331 bp the results showed that ill yearling pigeons recording high infectivity rate (66.7%)compare with healthy yearling pigeons and adult once, the bursa of Fabricius samples of dead yearling pigeons recorded high prevalence (36.58%) when compare with liver and spleen samples, DNA of pigeon circovirus high detected (60.71%) in bursa of Fabricius of dead in shell pigeon embryo. inconclusion pigeon circovirus affected the racing pigeon in Mosul, Iraq.

Development of in-house Taqman qPCR assay to detect equine herpesvirus-2 in Al-Qadisiyah city

Mohammed H. Al-Saadi

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 365-371
DOI: 10.33899/ijvs.2019.126076.1229

EHV-2 is distributed in horses globally. It is clustered within gamma-herpesvirus subfamily and percavirus genus. EHV-2 infection has two phases: latent and lytic. In the later, EHV-2 mainly associated with respiratory and genital symptoms. However, in the quiescent phase of infection, EHV-2 stay dormant in the host till viral reactivation. Our previous study has showed that EHV-2 can be harboured by equine tendons, suggesting that leukocytes possibly carrying EHV-2 for the systemic dissemination. So far, numerous PCR protocols have been performed targeting the gB gene. However, this gene is heterogenic. Therefore, there is a need to develop a quantitative diagnostic approach to detect the quiescent EHV-2 strains. To do this, Taqman qPCR assay was developed to quantify the virus. This was performed by targeting a highly conserved gene known as DNA polymerase (DPOL) gene using constructed plasmid as a standard curve calibrator. The obtained results showed an infection frequency of 33% in which the EHV-2 load reached 6647 copies/100 ng DNA whereas the minimum load revealed as 2 copies/100 ng DNA. The median quantification was found as 141 copies/ 100 ng DNA. Establishment of a credited qPCR assay to quantify EHV-2 could be helpful in the control of the disease.

Prevalence of the bovine adenovirus type 3 by using direct fluorescent antibody technique in calves in Nineveh province

Abdulhakeem A. Sheet; Safwan Y. Al-Baroodi

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 53-57
DOI: 10.33899/ijvs.2019.125476.1009

A total of 200 samples were collected from the calves for different ages from local and exotic breed by using nasal swabs, to investigate the prevalence of the bovine adenoviruses. The results showed that about 44% of the examined calves gave positive to immunofluorescence test, and the calves aged 6-9 months showed highly significant prevalence compare with other ages. The prevalence of virus infection in the exotic breed was 50.3% compared with local breed 22.2%. The calves with respiratory affections showed a higher prevalence from those which seem to be healthy. The current study concluded that the bovine adenovirus-type 3-virus has an effect on the breeding of calves in Nineveh Governorate.