Main Subjects : Animal Immunology

The inhibitory role of effective microorganisms on the growth of pathogenic bacteria

Mohammad A. Hamad; Saba A. Hussein; Ebtehal N. Mahmmoud; Ammar M. Al-AAlim

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 153-158
DOI: 10.33899/ijvs.2019.125653.1123

This study was conducted to evaluate the efficacy of Effective Microorganisms (EM1®) for inhibiting the growth of some pathogenic bacteria Staphylococcus aureus and E. coli were used in this study and isolated from pathological conditions. These bacteria were diagnosed in laboratory of microbiology, College of Veterinary Medicine, University of Mosul. The colonies that taken from blood agar were 5-7 and cultured in the nutrient broth and incubated at 37 ºC for 24 hours. Bacterial growth was calibrated with the second tube of the McFarland tubes 0.5%. Several concentrations of EM product were prepared 1, 0.5, 0.25 and 0.125%. Decimal dilutions were done for each concentration of EM product with bacterial suspension, except control group was done for bacterial suspension with nutrient broth. The bacterial count was done on nutrient agar, milk agar and EMB agar. The results of this study showed that the product of EM1® within concentrations 0.5-1% was highly efficient in inhibiting the growth of pathogenic bacteria under study. The bacterial count of both S. aureus and E. coli was 54x107 and 52x107 CFU/ ml respectively at 1% EM1®, and 67x107 and 86x107 CFU/ ml respectively at 0.5%, while the counting of the control group was 42x109 and 67x109 CFU/ ml respectively. This study concluded that EM1® at low concentrations have a clear role in inhibiting the growth of pathogenic bacteria, particularly S. aureus and E. coli.

Potency Syzygium cumini L as adjuvant therapy on mice model malaria

L. Maslachah; R. Sugihartuti

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 73-80
DOI: 10.33899/ijvs.2018.153801

The objective of the study was to prove the potential extract of Syzygium cumini L leaf and stem bark as an adjuvant to malaria modelling mice. Antimalarial effects were assessed by the percentage of parasitemia, growth inhibition, 50% dose level (ED50), Parasite Clearance Time (PCT), Recrudescence Time (RT) of Plasmodium berghei. Male albino Swiss mice infected with 1x105 P. berghei parasite in 0.2 ml intraperitoneally. Treatment with chloroquine 25 mg/kg body weight, chloroquine combination 25 mg/kg body weight with leaf and stem bark extract of Syzygium cumini L dose 600 mg / kg body weight for 4 days and 24 hours after infection, and then its activity as antimalarial and adjuvant therapy were observed. The results showed that the extract of Syzygium cumini L leaf combined with chloroquine gives highly significant result in inhibiting the growth of parasites than the chloroquine alone and the extract of Syzygium cumini L leaf combined with chloroquine gives the Parasite Clearance Time faster and Recrudescence Time (RT) longer than the other treatment.