Main Subjects : Animal Histology


The effect of CO-Q10 on the testicular histological changes in rats induced by imatinib

Luma K. Al-Allaf; Hafidh A. Al-Ashoo

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126587.1347

The safety zone of imatinib, and specifically its relevancy to organ toxicity, has been discussed dialectically in current years. Oxidative stress may be one of the causes of imatinib -mediated toxicity. This study aimed to examine the possible role of co-enzyme Q10 in ameliorating the adverse effect of imatinib on the testicular histology of male albino rats -if it is present. Twenty-eight male Albino rats were used randomly assigned to 4 experimental groups: Group 1 includes 40-45 days aged rats (n=8) which were gavage a dose of 200 mg/kg/day/30 days of imatinib mesylate. Group 2 includes age matched control rats which were administered with distilled water(n=4). Group 3 includes eight rats were received Q10, 50mg/kg, alone, Q10-sorb capsule of 50 mg given with the same schedule. Group 4. Includes eight rats were co administrated orally with 50 mg/kg of Q10+200 mg/kg of imatinib (once/day/30days). Euthanizing of animals with ether 24 hours after the final dose was done. Testes of rats from each experimental group were obtained. The tissues processed and stained by routine histological method. Histological sections of testes's rats treated with 200mg/Kg of imatinib revealed different testicular lesions compared to those of control group (P<0.05). Six 6(75%) of these sections revealed degenerated tubules, detached Sertoli cells, and apoptosis. These histological sections also showed thick tunica albuginea, seminiferous tubules with thick basement membrane. sometimes only a few of Sertoli cells were appeared in histological sections of imatinib treated rats. Mean Johnsen’s scores in these sections was 5.1±0.1 (P˂0.001). Features of retained spermatid were also noticed in some sections. There was significant reduction in both seminiferous tubular diameter and the epithelial height of histological sections of group 2(P˂0.001) with mean of 140.2±3.2µm and 14.8±1.1 µm respectively. Moreover, the number of Sertoli cells/ seminiferous tubule were significantly increased (P˂0.001), with mean of 27.4±0.2 and Leydig cell number is also significantly raised with mean of 7.5±0.5. In conclusion, treatment of peripubertal rats with imatinib induced several testicular alterations (including Sertoli cells) in comparison to control rats indicated that this drug is a gonadotoxic agent as it affects the quality and quantity of spermatogenesis. An Ameliorating effect of co-enzyme Q10 co-administration on imatinib-induced testicular toxicity was concluded.

Development of the wing bones in quail’s embryo; Coturnix japonica

Hadia K. Zorab; Kamal A. Salih

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126438.1324

Quail is an essential model in avian research because of economic importance in poultry industries around the world. Furthermore, its use in the experimental embryology research field. Therefore, this study aimed to identify the onset of chondrification and ossification of the wing bones also to summarize the main histological sequences related to the formation of the humerus in Japanese quail. Six embryos were taken every 24 hrs from 3-16 days of incubation. Three embryos were prepared and stained with Alcian blue and Alizarin red for cartilage and bone, respectively. The other three embryos have been processed for histological examination. The Macroscopical findings showed that the humerus, radius, ulna, coracoid, scapula, third, and fourth metacarpals were chondrified on 5th day. However, first signs of ossification were observed in the humerus, radius, and ulna on 8th day. While the minor digit remained none ossified at prehatching. The microscopical stages of developing humerus associated with the limb bud, apical ectodermal ridge formation, and chondrocyte differentiation on 3rd, 4th, and 5th day, respectively. The cartilage template of the humerus was established on 6th day. The diaphysis and epiphyses of the humerus were formed on 7th and 8th day, correspondingly. The periosteal-bone collar was formed on 8th day, and vascularization of chondroepiphysis has occurred on 9th day. There was a difference in the timing of chondrification and ossification in the forelimb skeleton and humerus developed by the endochondral mechanism. The obtained results should be considered in teratological and molecular studies in skeletogenesis.

Histological and antimicrobial effects of tramadol infiltration on incisional oral mucosal wound healing in rabbits

F.A. Al-Mashhadane; E.A. Mustafa; G.A. Taqa

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 335-340
DOI: 10.33899/ijvs.2019.162996

The present study compared effects of tramadol with lidocaine on healing processes of incisional intraoral mucosal wounds in rabbits and to study the antimicrobial effect of tramadol. For this purpose, fifteen apparently healthy male rabbits of 5-6 months old were used. They were classified into three groups, first group treated with 0.5 ml distilled water infiltration as control (n=5), second group treated with 0.5 ml tramadol infiltration in dose of 100 mg/2 ml (n=5), and third group treated with 0.5 ml of 2% vasoconstrictor-free lidocaine infiltration, then an incision was done to the buccal mucosa of all animals and they slaughtered on day 10 and their wounds were evaluated by histopathologic for presence of edema, vascularity, and inflammatory cells. For antimicrobial effect, swabs from tramadol group were taken from sites of wounds before incision and other swabs were taken after surgical incision on the first day, third day and seventh day, then the colony forming units (CFU)/ml of microorganisms were counted. The present study found that the infiltration with tramadol in dose of 100mg/2ml result in new epithelialization and wound healing occurred faster than infiltration with lidocaine 2% group also this study found that tramadol has significant decrease in the means of colony forming units of oral microorganisms. This study demonstrated that tramadol accelerated stages of buccal wound healing faster than lidocaine in rabbits.