Main Subjects : Veterinary Viruses

Phylogenetic tree analysis study of bovine papillomaviruses type 1 based on L1 gene in Al-Qadisiyah governorate, Iraq

Khalefa Ali Mansour; Hassan Hachim Naser; Muthanna Hadi Hussain

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 1, Pages 151-155
DOI: 10.33899/ijvs.2019.125535.1057

Bovine fibropapilloma and papilloma occur in different parts of the skin of animals. Bovine Papillomavirus (BPV) is an oncogenic virus making benign tumor lesion of together mucosal and cutaneous tissue in cattle. In order to confirm the clinical diagnosis; the study planned to make the molecular detection of BPV (DNA) using Polymerase Chain Reaction (PCR) from skin lesions and the phylogenetic analysis. Thirty-eight samples of skin lesions were collected from cattle clinically suspected to be infected with bovine papilloma virus from herds in Al-Qadisiyah Governorate in 2016, the primary clinical diagnosis depended on the morphological appearance and features of the lesion. Deoxyribonucleic Acid (DNA) was extracted from skin lesions; the DNA was examined by PCR technique using specific primer to BPV-1 /L gene-1. Twenty-two samples out of 38 (57,9%), which were collected from different regions in Al-Qadisiyah Governorate, were positive. The sequences of four positive samples of DNA product amplification of (BPV) type-1, L1 gene confirmed the PCR results. These samples had the DNA presented in four accession numbers KY662042-1, KY662043-1, KY662040-1 and KY662041-1. This study proofed that cutaneous bovine papillomatosis related with BPV-1 infection in the cattle herds has affinity to solid skin rather than other epithelial and mucosal tissue.

First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province/ Iraq

Salah Mahdi Karim; Khalefa Ali Mansour; Ali Hassan Janabi; Nawras K. M. Al-Nakeeb

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 1, Pages 123-126
DOI: 10.33899/ijvs.2019.125525.1047

This study was initiated for the first time for identification, using sequencing and phylogenetic analyses, of pseudocowpox PCPV that inhabit dairy cows in Al-Qadisiyah province, Iraq. Scab sampling was performed to obtain specimens from udder and teats of 18 affected cows. Initially, a polymerase chain reaction (PCR) method was followed to target a 408-bp piece of the GM_CSF/IL-2 inhibition factor gene (GIF) that belongs to PCPV. Then, the PCR products were sent out to partial sequencing of the GIF gene. The results of the PCR have indicated the presence of the virus in only 3 out of 18 samples. When the sequences were studied using phylogeny, the results have revealed that one of our PCPV strains has a close matching with some of the world strains such as from New Zealand. While two of the current study strains have clustered together with a strain from Finland. The results of our study confirm the presence of the PCPV in dairy cows that induces milker’s nodules.

Molecular diagnosis and genetic relationship of foot and mouth disease virus serotype Asia1/Basne/Sul/2015

Jeza Muhamad Abdul aziz; Salih Ahmed Hama; Hawre Kamel Faraj

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 1, Pages 67-73
DOI: 10.33899/ijvs.2019.125519.1041

Foot and Mouth Disease (FMD) is the most economically important viral-induced livestock disease worldwide. From April to May of 2015, tongue epithelial tissue samples were collected from 36 cattle in six villages, which share the border with Iran. Samples were screened using RT-PCR to amplify a conserved region in the VP1 gene, and phylogenetic tree analysis was performed based on the VP1 nucleotide sequence results. Furthermore, the nucleotide sequence was converted to an amino acid sequence in order to detect similarities between the studied samples and those previously published in GenBank (NCBI). Epidemically, based on the amino acid residues, genetic similarity, and amino acid substitutions, the VP1 nucleotide sequences were determined to be close to a novel group, group VII, with 94% identity. The VP1 amino acid sequence analysis revealed a close relationship to the Asia/BAL/PAK/iso-2/2011 isolate (Accession no. JX435109), with 95.7% identity, which is not significantly different. Analysis of the studied samples revealed that the FMDV serotype Asia1 causing the outbreak in the Basne district belonged to group VII, which was introduced from the Balochistan province of Pakistan through illegal movement of animals from this region.

Molecular identification of peste des petits ruminants virus in wild goat and domestic small ruminants by real-time -PCR technique in Erbil-Iraq

E.P. Candlan; F.P. Khoran; L. Hana

Iraqi Journal of Veterinary Sciences, Volume 31, Issue 1, Pages 51-54
DOI: 10.33899/ijvs.2017.126710

In July 2010 outbreak was occurred in wild goat in Barzan, Sherwin mizzen and Mergasur in Kurdistan Region- Iraq. There were over 2700 deaths (both young and adult) during the period of July 2010 to October 2011. Based on the clinical signs and post-mortem findings, the involvement of peste des petits ruminants virus (PPRV) was suspected. This was confirmed by Real Time PCR technique using TaqMan®probes for the detection of Peste des petits ruminants. The results of Real-Time PCR for the 9 sample taken from 9 Wild goat there are 6 sample positive and 3 sample negative and 76 sample from domestic ruminants (sheep and goat) 63 samples was negative for PPR. This result confirms the diagnosis domestic ruminants in the region are routinely vaccinated with an attenuated vaccine based on the ‘Nigeria/75/1’ strain of PPRV.

Immunosuppressive effect of Marek's disease virus in broiler

Mozahim Yasen Al-Attar

Iraqi Journal of Veterinary Sciences, Volume 19, Issue 1, Pages 1-6
DOI: 10.33899/ijvs.2005.37262

تمت دراسة التأثیر المناعی المثبط للاصابة التجریبیة بفایروس مرض میرک فی فروج اللحم على مستوى المناعة المتکونة عند تلقیح حیوانات التجربة بلقاح مرض نیوکاسل وقد تم الکشف عن مستوى الأضداد المناعیة باستخدام اختبار تثبیط التلازن الدموی، حیث أظهرت نتائج هذا الاختبار انخفاض معیار الأضداد المتکونة ضد مرض نیوکاسل فی المجموعة الأولى المصابة تجریبیاً بفیروس مرض میرک والتی جرى تلقیحها بلقاح نیوکاسل بالمقارنة مع المجموعة الثانیة والتی اعطیت لقاح نیوکاسل فقط دون احداث الاصابة التجریبیة بفیروس مرض میرک، کما استخدم اختبار التحدی للتأکد من مستوى الحمایة التی أحدثها لقاح نیوکاسل حیث تبین أن هذا اللقاح أعطى حمایة بنسبة 50% فی المجموعة الأولى بینما کانت تلک النسبة 90% فی المجموعة الثانیة عند استخدام فیروس مرض نیوکاسل الضاری کجرعة التحدی لکلتا المجموعتین. وهذا یدل على أن الإصابة بفیروس مرض میرک فی الدواجن له تأثیر سلبی على مستوى المناعة التی یحدثها لقاح مرض نیوکاسل فی الدواجن.