Main Subjects : Veterinary Ectoparasite


Molecular identification of new circulating Hyalomma asiaticum asiaticum in the Sheep and goats in Duhok Province-Iraq

Shameeran Ismael; Lokman Tayib Omer

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126330.1298

Abstract
Ticks are obligate ectoparsite that infest both animal and human and causing many diseases, due to they act as a vector of some pathogens including: protozoa, bacteria and virus. There are about 877-878 tick species; most of these are under the two famous families including: Ixodidae and Argasidae. Because there was no such study done on identification of tick species by PCR technique in Kurdistan and particularly in Duhok Governorate, therefore present study was done to identify tick species by using molecular study by using of 16S rRNA and DNA sequencing. About 1000 ticks were collected from both sheep and goat respectively (500 and 500), form Duhok Governorate including: Barwaria, Zakho, Sumeil, Mangeshik, Sersin, Shekhan and Akre, Iraqi Kurdistan, between May and June 2016, between April and June 2017. The result found during this study were six species under two genera of the hard ticks were identified by molecular study and sequencing including: three species were under the genus Hyalomma and three species were under the genus Rhipiciphalus that infect small ruminants in Duhok Governorate from these species a new species under the Hylomma genra (Hyalomma asiatium asiaticum) with accession number (MN594484), was first time reported in Duhok Governorat, Iraqi and in Kurdistan. Also phylogenetic tree was constructed depend on the 16S rRNA. More advanced molecular study are need such as the PCR – RFLP method will be more accurate molecular tool for ticks differentiating of Duhok and will be helpful in epidemiological and geographical distribution .

Synergizing the deltamethrin larvicidal activity against Aedes albopictus larvae using cinnamaldehyde in Diwaniyah, Iraq

Mansour J. Ali; Azhar C. Karawan; Dhafer R. Al-Fetly; Monyer A. Alfatlawi

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 317-320
DOI: 10.33899/ijvs.2019.126026.1212

The current work on mosquito larvae was performed to evaluate the resistance status of larvae to deltamethrin (DM) and to detect if the larvicidal activity (LA) of this chemical could be synergized after exposing the larvae to cinnamaldehyde (CD). Here, 200 Aedes albopictus larvae were employed for the experiment and were divided randomly into 2 groups (100/each group and placed in petri-dishes (PD), 10 larvae/PD), and they are the DM group (1ml of 0.04 mg/l in 99ml of distilled water (DW) was placed to each PD) and the DM+CD group (1ml of 0.04 mg/l and 1ml of 0.9mg/l respectively were placed with 98ml DW in each PD). The experiment was lasted for 24hrs. Larvae were detected to have resistance against DM as 45% to 60% of the larvae were killed by the DM, 40% to 55% resistance rate. However, when evaluating DM activity with the use of CD, the LA was synergized showing mortality in 87% to 92% of the larvae in which a significant increase in the mortality in DM+CD group was noticed more than that in the DM group. Furthermore, RT-qPCR was run to identify the expression status of the P540 monooxygenase gene, Cyp6p15, and found that the gene expression was significantly inhibited in the DM+CD group when comparing that in the DM group that showed overexpression of this gene. This work results provide viable information about the potential activity of the cinnamaldehyde in synergizing the larvicidal activity of deltamethrin.