Main Subjects : Veterinary Protozoa


Molecular Detection Of Hammondia heydorni in Dogs In Mosul city

Wasan Amjad Alobaidii

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2019.126052.1219

Through this study 122 individual fecal samples was collected from dogs , with different ages ,sexes, and breeding management ( stray and pet house hold ). some dogs were with healthy appearances while others were suffered from diarrhea ,anorexia ,depression , dehydration. Infection was investigated by light microscope after flotation technique by using Sheather's sugar solution. if the sample gives positive to oocyst (H. heydorni or other relative group),were mixed with potassium dichromate solution a rate of 2.5% for the stimulation of sporulation. DNA extraction was done using 2% sarcosyl ,pronase E followed by phenol/chloroform extraction then precipitation attempt using ethanol. DNA amplification was attempt using H. heydorni primers JS4 and JS5 n microscope examination give 78 sample positive to the presence of oocyst , while polymerase chain reaction showed total percentage of infection with H.hydorni was 34.6% ( 27 case give positive reaction to PCR). Dogs less than 6 months of age show high infective rate (17.9%), dogs which suffer from gastrointestinal tract troubles (diarrhea , anorexia and dehydration) gave high infective rate (24.4%). Stray dogs gave high prevalence of infection to H .hydoeni (25.6%) when compared with pet house hold dogs which recorded (9%) percentage of infection. This study is the first recorded H.hydorni in dogs in Mosul city.

Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province

Noor Jarad

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2019.126159.1249

This study were conducted in Al-Diwaniya province, in south Iraq during the period from February to July (2019) to determine the rate of infection of Cryptosporidium parvum in domestic chicken, study the effect of some epidemiological factors such as sex and months on the rate of infection, addition to the molecular Identification of Cryptococcus parvum by amplification HSP70 gene by conventional PCR for first time in Iraq. Number of collected fecal sample was (210) from domestic chicken and processed by Ziehl-Neelsen method. The results of the microscopic examination showed that 108 (51.4 %) out of 210 fecal samples were infected with Cryptosporidium spp. The statistical analysis founded no marked difference in prevalence of infection between sexes. Significant difference (P<0.05) was recorded between infection rate during the months of the study and higher prevalence of infection rate was observed in March (11.9%), while lowest infection rate was observed in July (5.23%). and June (5.23%). Genomic DNA was Genomic DNA was extracted from 108 fecal samples and HSP70 gene for C. parvum was amplified by polymerase chain reaction (PCR). PCR technique is showed that out of 108 fecal samples (21.3%) were positive for C.parvum.

Molecular study to detect the Eimeria Species in Sheep in Al-Diwaniyah province / Iraq

Noaman Naji A&amp;#039;aiz; Noora Majeed; Ahmed Neama

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2019.126064.1225

Sheep eimriosis is one of the most important and common disease which infects sheep in all ages but it is more effective in lambs. The diarrhea with or without blood is the main signs of infection. Eimeria protozoan required single host to complete its life cycle which pass in different stages including schizogony, gametogony and sporogony. The study was designed for detection of sheep Eimeria species through the molecular method.
This study was conducted in Al-Diwanyah province during the winter months of 2019. In which 200 sheep fecal samples were collected and examined traditionally to investigate the Eimeria oocytsts. Ninety seven samples of highly intensity infection with Eimeria oocysts were selected to subject for DNA extraction process. The extracted DNAs were tested through amplification of internal transcribed spacer 1 (ITS-1) gene by conventional PCR, and then phylogenetic analysis was made to diagnose the sheep Eimeria species.
All samples that examined microscopically were showed positive results of infections with Eimeria protozoan. Out of 97 molecularly examined samples, forty five (46.39%) were given positive result in conventional PCR technique, where Eimeria spp. detected through succeeded amplification of internal transcribed spacer 1 (ITS-1) gene. Then phylogenetic analysis referred to that there are five species of Eimeria confirmed in sheep in Al-Diwanyah province including 6 (33.33%) samples diagnosed as E. ahsata , 4 (22.22%) samples E. weybridgensis, 3 (16.66%) samples E. ovinoidalis, 3 (16.66%) samples E. bovis and 2 (11.11%) samples E. auburnensis.

Microscopic identification, molecular and phylogenetic analysis of Babesia species in buffalo from slaughter house in Al-Najaf city of Iraq

Rashaa Ateaa; Mansour Alkhaled

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 251-258
DOI: 10.33899/ijvs.2019.162882

Babesia is one of hemoprotozoan parasite transmitted by arthropod vectors which responsible for causing of Babesiosis disease in bovine worldwide. The present study was designed for microscopic identification, molecular, and phylogenetic analysis of Babesia species in buffalo from slaughter house in Al-Najaf city of Iraq. The study performed in three months of summer season (August into September 2017) and animals ages and sex were included in this study. The direct microscopic prevalence results were show highest prevalence of haemoprotozoa prevalence at Babesia sp. 45.74%. The prevalence of Babesia sp. related to animal sex, were show in male 43.48% and female was 52%, with non-significant differences. The Prevalence of Babesia sp. related to age were show 12.50%, 92.86% and 30% in young, adult and old age respectively with significant differences (P<0.05). The prevalence of Babesia sp. related to month of study were show. 28.57%, 62.50% and 42.86 in August, September and October respectively and with non-significant differences. Molecular study results were based on PCR and DNA sequencing method by phylogenetic tree analysis (MEGA 6.0) and NCBI-BLAST Homology Sequence Identity to differentiation Babesia species typing. The Babesia species prevalence results were show identified two Babesia species, high prevalence of Babesia bovis (38.30%) were closed related to NCBI-Blast Babesia bovis (HQ264126.1) with homology sequence identity 97-100% and Babesia bigemina 7.45% were closed related to NCBI-Blast Babesia bigemina (KU206291.1) with homology sequence identity 95-99%, then 43 Babesia species includes (B. bovis and B. bigemina) were submitted into NCBI-Genbank and provided accession numbers (MH503811-MH503853). In conclusion, this study concluded that Phylogenetic tree and homology sequences identity was show accurate in differentiation of Babesia species, and these species can be isolated at from local water buffalo from slaughter house in Al-Najaf city, of Iraq.

Microscopic study for prevalence of Babesia bovis and Babesia bigemina in cattle in Mosul city

A.F. Altaee; E.G. Suleiman

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 2, Pages 57-66
DOI: 10.33899/ijvs.2017.145579

The current study included examination of Babesia bovis and Babesia bigemina in 300 blood smears stained with 5% of Giemsa stain which collected from cattle in Mosul city from both sexes with different ages, the total percentage of infection with Babesia spp was 42.33% and the percentage of B. bovis and B. bigemina 30.66% and 24.33%. The blood smears which stained with Giemsa stain contain 0.5% Triton X-100 appeared cleaner and easily recognized the blood cells and parasites. High percentage of infection was appeared with single infection with B. bovis was 42.51% followed by mixed infection with B. bovis and B. bigemina and infection with B. bigemina only was 29.92% and 27.55%. The results of this study showed no significant differences in the percentage of infection between males and females of cattle and different groups of ages at the significant level p<0.05.

Detection the some developmental stages of Babesia spp in hemolymph and midgut of adult females of Boophilus microplus naturally on cattle in Mosul city

E.G. Suleiman; A.F. Altaee

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 2, Pages 67-75
DOI: 10.33899/ijvs.2017.145600

This study involved the detection of vermicules of Babesia spp in the hemolymph of Boophilus microplus adult females naturally fed on cattle after their remaining a live for 5 to 8 days under laboratory conditions and the detection of some developmental stages (schizonts, rupture of schizonts, small dark collar bodies and migration of vermicules from the hemolymph to different tissues of tick). Both vermicules of B. bovis and B. bigemina were diagnosed. The vermivules of B. bovis appeared as croissant or club-shaped bodies with a broad anterior ending and pointed posterior one and having a central nucleus and a vacuole in the anterior end with a curved or semi curved tail. The vermicules of B. bigemina had a croissant or club –shaped bodies with a lesser width in their anterior end than in B. bovis with no vacuole and with a straight tail. The average length of B. bovis was 13.92 ±1.34 µ with a range of 11.8-15.8µ and average width of 3.23±0.44 µ with a range of 2.7-4µ. The average length of B. bigemina was 11.39±1.12µ with a range of 9.5-13µ and average width of 2.2±0.51µ with a range of 1.5-3µ. The results of current study revealed that their was a direct and proportional relationship between the parasitemia of B. bovis and B. bigemina in cattle blood smears and the number of vermicules in the hemolymph of female of Boophilus microplus. No significant differences were noticed between (number and percentage of Boophilus microplus females) infected with vermicules of Babesia spp at different aged cattle.

Prevalence of Anaplasma ovis infection in Angora goats of Duhok province, Kurdistan region-Iraq

I.A. Naqid

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 2, Pages 73-79
DOI: 10.33899/ijvs.2017.145599

Acute Anaplasma ovis infections can cause severe clinical symptoms and might lead to significant economic losses in small ruminant flocks. However, little information has been provided related the prevalence of anaplasmosis in Angora goats. The study was designed to investigate the prevalence of Anaplasma ovis serologically (cELISA) and microscopically (Giemsa stained blood smears) among Angora goats from Duhok districts of the northern part of Iraq. A total of 92 blood samples were randomly collected from three localities of Duhok city; Zakho, Batel and Sumil during the study period from April to October 2009. The infection rate of A. ovis was 38.04% by Giemsa stained blood smear and 66.3% by cELISA. The prevalence of A. ovis in female goats was higher than that in males, but statistically not significant difference (P>0.05) by using both methods. The prevalence was also significantly higher (P<0.05) in goats more than three years old than in younger ones. The highest prevalence of A. ovis was found in Zakho, whereas the lowest was reported in Sumail. Results of hematological parameters indicated microcytic hypochromic type of anemia.It is concluded that A. ovis can infected Angora goats in district Duhok, Kurdistan region, Iraq and this might be due to high distribution of the disease and its transmitters which were lead to substantial effect followed by high mortalities