Veterinary Microbiology
Multi-drug resistant phenotypes of ESBL-producing Escherichia coli from layer chickens

Wale Olopade; Asinamai Athliamai Bitrus; Asabe Halimat Momoh-Zekeri; Pwaveno Huladeino Bamaiyi

Articles in Press, Accepted Manuscript, Available Online from 03 August 2022

Abstract
  Antimicrobial resistance (AMR) is recognized as a growing and emerging public health problem worldwide. This research determines the occurrence of ESBL E. coli and antimicrobial resistance profiles of Escherichia coli on eggshells from selected layer chickens. The shells of 270 egg samples were swabbed ...  Read More

Veterinary Microbiology
Detection of Campylobacter fetus in aborted ewes in Sulaimani province by PCR

Eman D. Arif

Volume 36, Issue 3 , June 2022, , Pages 647-651

Abstract
  Abortion is one of the most critical factors affecting lambing rates and, as a result, sheep farm profitability. It is also significant from a zoonotic viewpoint, in addition to financial losses. In sheep flocks, Campylobacter fetus causes infectious infertility, embryonic death, and miscarriages. The ...  Read More

Veterinary Microbiology
Control of biofilm-producing Aeromonas bacteria in the water tanks and drinkers of broiler poultry farms using chitosan nanoparticle-based coating thyme oil

Asmaa N. Mohammed; Amira S. Attia

Volume 36, Issue 3 , June 2022, , Pages 659-669

Abstract
  In a drinking water distribution system, biofilm-producing bacteria are considered an alarm bell for increased emergence of waterborne pathogens. This study aims to monitor the prevalence of biofilm-forming Aeromonas species in the drinking water distribution systems in different broiler chicken farms. ...  Read More

Veterinary Microbiology
Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study)

Maimonah Q. Yahya; Zahraa Khairi-Aldeen Mohi-Aldeen

Volume 36, Issue 3 , June 2022, , Pages 761-768

Abstract
  Isolation and identificationof the bacteria-infected hydatid cystsof livestock animals were carried out. The study lasted for five months. A total of 302 slaughtered livestock were checked to find hydatid cyst naturally infected livers and lungs of sheep and goat at local Mosul abattoir/ Nineveh/ Iraq. ...  Read More

Veterinary Microbiology
Phenotypic characterization and antibiogram of extended spectrum ß-lactamase (ESBL)/AmpC-producing Escherichia coli isolated from sheep

Fatma R. Mahmood; Ihsan M. Ahmed

Volume 36, Issue 2 , April 2022, , Pages 303-307

Abstract
  This study aimed to isolate and characterize extended-spectrum ß-lactamases (ESBLs) and AmpC producing E. coli in sheep in Mosul city. A total of 260 milk and fecal samples were collected aseptically from healthy ewes (n=60), their respective lambs (n=60), and ewes with clinical mastitis (n=40). ...  Read More

Veterinary Microbiology
Molecular characterization of extended spectrum beta-lactamase producing Klebsiella pneumoniae isolated from cows in Mosul city, Iraq

Sumaya Y. Aldabbagh

Volume 36, Issue 2 , April 2022, , Pages 375-380

Abstract
  Cows are considered as reservoir hosts for many pathogenic bacteria that are resistant to broad-spectrum beta lactamase(ESBL). Presence of ESBL resistant K. pneumoniae in nasal of cows and beef meat constitutes a risk for public health due to transfer of antibiotic resistance gene from cows to environment, ...  Read More

Veterinary Microbiology
Molecular detection of ESBL/AmpC ß-Lactamase Escherichia coli isolated from sheep in Mosul city

Fatma R. Rafee Mahmood; Ihsan M. Ahmed

Volume 36, Issue 2 , April 2022, , Pages 387-392

Abstract
  Globally, extended-spectrum ß-lactamase (ESBL)/Ampicillin ß-lactamase (AmpC) producing Escherichia coli has become the greatest threat for distributing antibiotic resistance. Accordingly, this study was designed to detect and screen the genes that confer resistance in E. coli isolated from ...  Read More

Veterinary Microbiology
Some immunological responses in rats injected with prepared bacterin toxoid of local methicillin resistant Staphylococcus aureus

Abdullah A. Abdulazeez Sheehan; Mohammed H. Khudor; Fanar A. Isihak

Volume 36, Issue 2 , April 2022, , Pages 401-406

Abstract
  This study was aimed to prepare bacterin toxoid from local isolate of methicillin resistant Staphylococcus aureus (MRSA) isolated from bovine mastitis in Basrah province. Isolated MRSA was detected and confirmed using polymerase chain reaction. Then inactivated bacterin toxoid was prepared. A total of ...  Read More

Veterinary Microbiology
Extraction and purification of lipopolysaccharide from Escherichia coli (local Isolate) and study its pyrogenic activity

Ammar M. Al-AAlim; Ali A. Al-Iedani; Mohammad A. Hamad

Volume 36, Issue 1 , January 2022, , Pages 45-51

Abstract
  In this study, we tried to extract and purify the LPS from E. coli local isolate and determine the molecular weight, purity, and pyrogenic effect of the product and compare it with standard E. coli O55:B5 LPS, the E. coli LPS was extracted by using hot phenol method then SDS- PAGE was used with both ...  Read More

Veterinary Microbiology
Major-surface-protein-4-gene-based detection of Anaplasma marginale isolated from sheep in Al-Diwaniyah province, Iraq

Saba F. Klaif; Asaad J. Abid; Monyer A. Al-Fatlawi; Mansoor J. Ali

Volume 36, Issue 1 , January 2022, , Pages 85-88

Abstract
  This study was purposed for confirming detection and typing of Anaplasma spp in infected sheep from Al-Diwaniyah province, Iraq. Sampling of 50 blood specimens was performed from clinically-identified infection of anaplasmosis. The samples of the blood were subjected to DNA extraction followed by polymerase-chain-reaction ...  Read More

Veterinary Microbiology
Molecular characterization and phylogenetic analysis of Escherichia coli isolated from milk of cattle affected by mastitis

Azhar A. Neamah; Khilood H. Fahad; Jenan N. Sadeq; Monyer A. Al-Fatlawi

Volume 36, Issue 1 , January 2022, , Pages 251-254

Abstract
  This evolution-based study aimed to reliably identify the epidemiological prevalence of Escherichia coli that wasrecovered from affected milk of cattle by mastitis, study the evolution of this bacterium, and describe some isolates using polymerase chain reaction (PCR) technique and DNA sequencing. Here, ...  Read More

Veterinary Microbiology
Multi-drug resistance profile of extended spectrum β-Lactamases producing Escherichia coli isolated from sheep in Mosul city

Fatma R. Mahmood; Ihsan M. Ahmed

Volume 35, Supplement I-III , December 2021, , Pages 37-40

Abstract
  Multi-drug resistance (MDR) Escherichia coli have become a major threat due to their ability to overcome different types of antibiotics. However, Extended Spectrum β-lactamase E. coli (ESBLE) imposes an additional threat due to their ability to resist the 3rd generation cephalosporins. Accordingly, ...  Read More

Veterinary Microbiology
Isolation and antimicrobial resistance of Staphylococcus spp., enteric bacteria and Pseudomonas spp. associated with respiratory tract infections of sheep

Teba A. Aziz; Inam J. Lafta

Volume 35, Supplement I-III , December 2021, , Pages 53-58

Abstract
  Sheep are considered as an important part of livestock in the worldwide, particularly in Iraq, as they provide meat, milk, leather, wool, and manure. The present study aim is isolation and identification of staphylococci, enteric bacteria and Pseudomonas spp. Totally, 115 samples were collected from ...  Read More

Veterinary Microbiology
Isolation and molecular detection of enterotoxigenic Staphylococcus aureus from raw milk of cows

Dunya D. Taher; Safana A. Yassin; Mariam H. Abdulkareem

Volume 35, Supplement I-III , December 2021, , Pages 137-141

Abstract
  Staphylococcus aureus is a microbe associated with human’s food poisoning. It is caused by isolates producing different heat stable enterotoxins which act as one of the most spread worldwide gastroenteritis causes. The goal of the current study was to assess the incidence of S. aureus (enterotoxigenic) ...  Read More

Veterinary Microbiology
Immunotherapeutic effect of chitosan and listeriolysin O on Listeria monocytogenes infection in mice

Hero I. Ali; Shaimaa N. Yasssein

Volume 35, Supplement I-III , December 2021, , Pages 149-155

Abstract
  This study aimed to evaluate the effect of dietary chitosan and listeriolysin O (LLO) on the immune response against Listeria monocytogenes infection in mice. L. monocytogenes isolate was obtained from the unit of Zoonotic Disease, College of Veterinary Medicine, University of Baghdad. It was reactivated ...  Read More

Veterinary Microbiology
Genetic diversity and clonal relatedness of Aeromonas hydrophila strains isolated from hemorrhagic septicemia’s cases in common Carp (Cyprinus carpio) farms

Zanan M. Taha

Volume 35, Issue 4 , October 2021, , Pages 643-648

Abstract
  The objective of this study was to determine molecular typing and comparison analysis of 24 Aeromonas hydrophila isolated from the diseased fish with hemorrhagic septicemia in freshwater ponds and cage in Mosul and Duhok cities, Iraq. A total of 24 A. hydrophila isolates that were collected from various ...  Read More

Veterinary Microbiology
Molecular characterization of extended spectrum cephalosporin resistant Escherichia coli isolated from dogs

Ihsan M. Ahmed; Sumaya Y. Aldabbagh; Dhyaa M. Jwher

Volume 35, Issue 3 , July 2021, , Pages 473-478

Abstract
  Shepherd dogs have been implemented in the transmission and distribution of many threatening pathogens. The presence of extended-spectrum-cephalosporin resistant Escherichia coli (ESCR E. coli) in dog feces can constitute a significance risk to human health due to transmission of antibiotics resistance ...  Read More

Veterinary Microbiology
Molecular analysis of ompA gene Pasteurella multocida Indonesia local isolates

A. T. Soelih Estoepangestie; Arini Rahmi Dewi; Suwarno Suwarno; Didik Handijatno; Rahaju Ernawati; Wiwiek Tyasningsih

Volume 35, Issue 2 , April 2021, , Pages 211-216

Abstract
  The aim of this research was to analyze ompA molecular gene of Pasteurella multocida buffalo isolate and bovine isolate from Nusa Tenggara Timur, Indonesia and Katha strain isolate from hemorrhagic septicemia vaccine. Determinant of P. multocida local isolates ompA ...  Read More

Veterinary Microbiology
Study the effect of cloned pET-32a(+) plasmid by Lysostaphin gene against Staphylococcus aureus

Jalal Yseen Mustafa

Volume 35, Issue 2 , April 2021, , Pages 233-238

Abstract
  Lysostaphin is a protein zinc metalloproteinase, extracted from Staphylococcus simulans, which disrupting peptide layer of S. aureus. In this study, Lysostaphin gene was detected in the S. simulans isolates. The molecular weight of the Lysostaphin gene is 750 bp. We were used the pET-32a(+) plasmid to ...  Read More

Veterinary Microbiology
Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms

Zanan M Taha; Shaaban Tayar Sadiq; Warheen Aziz Khalil; Kaheen Yusif Muhammad-Ali; Hayam Salih Yosif; Hozan Nizar Shamil

Volume 35, Issue 2 , April 2021, , Pages 375-380

Abstract
  The significance of Aeromonas hydrophila concerning hemorrhagic septicemia in aquaculture farms production in Duhok province, Iraq was investigated. Antibiotic-resistant profiles of isolates were also investigated with 8 antibiotics. Bacterial isolates were identified by using morphological and biochemical ...  Read More

Veterinary Microbiology
Detection of CTX-M gene in extended spectrum β-lactamases producing Enterobacteriaceae isolated from bovine milk

Ihsan M. Ahmed

Volume 35, Issue 2 , April 2021, , Pages 397-402

Abstract
  Extended spectrum β-lactamases producing Enterobacteriaceae (ESBL-E) have emerged recently as the main cause that facilitates the spreading of antibiotic resistance worldwide. Due to its composition and nutritive values, raw cow milk is vulnerable to bacterial contamination from different sources, ...  Read More

Veterinary Microbiology
Physiological effects of lactic acid bacteria against melamine induced toxicity in female albino rats

Ali H. Jameel; Mohammed J. Mohammed; Manal S. Mahdi; Karkaz M. Thalj

Volume 35, Issue 1 , January 2021, , Pages 1-7

Abstract
  The aim of this study was to investigate the ameliorative effect of two type of lactic acid bacteria Lactobacillus casei and Lactobacillus acidophilus against melamine toxicity by some physiological indicators in mature female rats after 21 days. In this study using 35 of female mature rats and divided ...  Read More

Veterinary Microbiology
Incidence of internal parasites of the slaughtered local breeds of ducks and geese

Baydaa Younis Al-lahaibi; Manal Himmadi Hasan; Ahlam Fathi Altaee

Volume 35, Issue 1 , January 2021, , Pages 39-44

Abstract
  This study was carried out to determine the presence of gastrointestinal parasites of local ducks and geese in Nineveh province. Sixty-four ducks and seventy geese of different ages and sexes were purchased from local markets. Necropsy findings in ducks reviled a total infection rate of 68.8% was with ...  Read More

Veterinary Microbiology
Evaluation the safety and synergistic effect of NiFe2O4 nanoparticles with antibiotic against Pseudomonas aeruginosa

Hala M. Majeed; Heba Younis Khalef; Halah Abdulkhaleq Awadh; Bashar Sadeq Noomi; Nihad Abdul-Hussain jafar; Khaild Ahmad Hadi

Volume 35, Issue 1 , January 2021, , Pages 71-77

Abstract
  The antimicrobial resistance currently impedes and threatens the future of effective prevention and treatment of the continually expanding range of infections caused by bacteria. This study aimed to identify the bacterial causes the wound infection among animals and using the antibiotic/nanoparticles ...  Read More

Veterinary Microbiology
Molecular fingerprinting of methicillin resistant Staphylococcus aureus strains isolated from human and poultry in Duhok, Iraq

Hishiyar A Hado; Mahde S Assafi

Volume 35, Issue 1 , January 2021, , Pages 99-103

Abstract
  Methicillin-resistant Staphylococcus aureus (MRSA) has been recently identified in poultry and farm workers. The aim of this work was to investigate the epidemiological relatedness of MRSA among chickens and farmworker. MRSA isolates (n=50) from human (n=14) and from chikens (n=36) were tested for molecular ...  Read More