Main Subjects : Reproduction Biotechnology

Effect of vitamin E and selenium injection on semen quality and some blood parameters of Karadi rams

Mohannad Mahdi Hameed; Abdulnasir Alkhashab

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 17-24
DOI: 10.33899/ijvs.2019.125554.1073

The Objective of this study was to determine the effect of vitamin E and selenium (Se) injection on semen characteristics and physiological and biochemical parameters of Karadi rams. fifteen Karadi Rams with average body weight 63 kg and 3-4 years of age were used in this study. The animals were randomly divided into three groups (5 rams / group) and the Rams were fed a standard diet equal in energy and protein and treated as follows. First control group, rams were injected with 1 ml normal saline once weekly. The second group, rams were injected with 2 ml (2.5 mg vitamin E plus 50 µg sodium selenite / kg body weight) once weekly. The third group rams were injected with 4 ml (5 mg vitamin E plus 100 µg sodium selenite /kg body weight) twice in week (2ml in one injection.) and the experiment continue for 3 months. Results revealed that treatment with vitamin E plus selenium Se led to a significant improvement (P≤0.05) on semen characteristics also a significant (P ≤ 0.05) increase in physiological and biochemical blood characters of Karadi Rams.

Role of spermatozoa in pH stability of caudal epididymis environment

Saleh W. Mahdi; Mahdi S. Al-Shamary; Zalzala S. Jaafir

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 111-116
DOI: 10.33899/ijvs.2019.125511.1033

50 ram testicles where been collected directly after slaughtering, preserved in cool box (8-10°C) and transferred to the andrological lab at the Veterinary Medicine Collage / University of Baghdad. Samples were washed thoroughly with distal water stripped from surrounding tissues. Epididymis were separated from the entire testicles, caudae were cut out and injected with warm normal saline then sliced, pH was collected daily (4-5 days). Result showed that: as the preservative periods proceed, the pH of caudal secretions is elevated as (6.22, 6.35, 6.45, 6.65, 6.66, 6.7, 6.77) at (24, 36, 48, 60, 72, 84, 96 hrs) while total motility percentage are (90, 85, 84.5, 77, 75, 75, 70%). Cold preservation after slaughtering showed decreased motility as preservation proceed as well as total alive spermatozoa decreased. In conclusion caudal secretion turned to be more acidic if cold preservation time is prolonged and total alive spermatozoa number decreased while dead one increased.

Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens

W.M. Saleh

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 1, Pages 55-65
DOI: 10.33899/ijvs.2017.126711

The aim of this study is designed to evaluate the best methods for cow oocytes collection from abattoir specimens which is the cheapest, easily obtained and bulky number. Forty five fresh cow genitalia specimens and testicle were collected directly after slaughter from Al-Shoáalla abattoir north-west of Baghdad the capital early morning, transported in cool box under (4-8) °C to the laboratory of theriogenology in the college of veterinary medicine/Baghdad University during the period from November 2016 to February 2017. Ovaries were separated from the surrounding tissues, washed thoroughly with dis. water repeatedly, then with normal saline and finally with MEM medium containing Antibiotics and Nystatin for contaminant elimination. Oocytes were collected with four methods aspiration, slashing, slicing after aspiration and slicing. The result showed that; the collected oocytes were 55, 68, 87 and 106 oocytes respectively; slicing methods yield more oocytes count. Period of time between slaughtering and samples processing significantly affect oocytes collected percentage and quality, periods as 2, 6, 12 and 24 hours yield 75%, 68%, 61% and 55% oocytes counts of good, fair, poor to aged and bad quality oocytes respectively. Two hours period yield an elevated oocytes count with good quality. Maturation index of oocytes according to the type of collected methods showed 44, 37, 39 and 42 with 12, 8, 6 and 6 good oocyte quality for the four methods respectively. In conclusion slicing methods yield more oocytes count with a moderate quality and embryos production while aspiration methods yield a moderate oocytes count with an elevated quality and good embryos production.

Examination of the reproductive system of cows using the ultrasonography

D.M. Aziz; I.H. Hamad

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 91-96
DOI: 10.33899/ijvs.2020.166879

The aim of this study was examination of normal and abnormal cow reproductive system using the ultrasonography. Twenty one Iraqi local breed cows were examined; 6 cows were normal with different phases of estrus cycle, 6 cows were pregnant at different stages, one cow at postpartum period, 4 cows suffering from metritis, 3 cows with pyometra and one cow having luteal cystic ovary. The cows were examined transrectally using the 7.5 MHz linear transducer. Results of study showed, that presence of small black spots on the ovary at proestrus which were indicated that presence of growing follicles, while the black area at the ovary was large at the estrus phase which was an indicator for mature follicles. The ovary of cow with luteal cystic ovary appears larger than normal ovary and has a large gray structure. The normal uterus appears with thin uterine wall and narrow uterine lumen, while in metritis the uterine wall was thicker about 2-5 times than normal uterus, also the thickness and ecogenisity of the cervix was increased. There was an excessive accumulation of fluid in the uterine cavity which was appearing in grayish color, while the accumulated fluid in cases of pyometra was unhomogenized and more ecogenic. Pregnancy diagnosis was very easy by using of ultrasonography, the pregnant uterus has a thin uterine wall and large black area which was the first indicator for pregnancy, also presence of caruncles was an indicator for pregnancy, while appearing of ecognic fetal parts was the best indicator for pregnancy diagnosis. We concluded that ultrasonography is a very useful tool for pregnancy diagnosis and reproductive disorders differentiation in cows.

Effect of reproductive season and semen collection on the concentration of some reproductive hormones of Awassi rams

Sh.H. Ali; M.B. Taha

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 75-81
DOI: 10.33899/ijvs.2020.166877

The study was conducted to estimate the concentration of Follicle Stimulating Hormone (FSH), Interstitial Cell Stimulating Hormone (ICSH) and Testosterone (T) in Awassi ram serum, the effect of reproductive seasons and semen collection on the concentration of these hormones. Six Awassi rams, aged 3-4 years and weighted 55-60 kg, were used in this study. Artificial vagina was used for semen collection every two weeks along the study period (July 2010 to February 2011). Semen samples were evaluated immediately after collection to determine volume, mass, individual motility and sperm concentration. Blood samples were collected before and after 30 min. of semen collection. The concentration of T was evaluated by ELISA, and concentration of FSH and ICSH were determined using the Immunofluorescence assay. Results showed that the average concentration of T, FSH and ICSH were 2.33 ± 0.22, 1.60 ± 0.20 and 0.53 ± 0.17 ng/ml, respectively. There was no significant variation between rams in all three determined hormones. The concentration of T and ICSH was highly significant (P<0.05) in autumn than in summer and winter. There was a significant (P<0.05) variation observed in concentration of T between summer and winter, while no variation in FSH concentration between the year seasons. The concentration of T during the reproductive season was highly significant (P<0.001) than its concentration out of season. There was a significant (P<0.001) increase in T concentration after semen collection. The concentration of T and ICSH has a positive relationship with ejaculate volume, and negative relationship with other semen parameters. The relationship was negative between FSH and sperm concentration and positive with other sperm parameters. Only a significant (P<0.05) negative correlation (r = -0.773) was observed between T and sperm concentration. The relationship was negative between T and ICSH and positive between T and FSH, and a significant (P<0.001) negative correlation (r = -0.663) was observed between ICSH and FSH. It be concluded that the higher sexual activity of Awassi rams was in autumn especially in October, and only the T was affected by reproductive season and increased after semen collection.