Main Subjects : Veterinary Obstetrics

Creatine kinase and C reactive protein as an indicator for tissue damage in the retained placenta in cows

Barra Al-Wataar; Eman Lazim; Osama H. Al-Hyani

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126496.1338

The current study aimed to investigate concentrations of Creatine Kinase (CK) and C Reactive Protein (CRP) in blood serum of cows with the retained placenta to compare with cows of normal parturition, also, to compare their concentrations in association with fetal sex, previous parturition and time to placenta expel. A total of sixty-three cows suffered from retained placenta with cows of normal parturition (n=10) were included in the current study during the period starting from December 2018 till September 2019. Blood samples (10 ml) were collected from the jugular vein to determine the concentration of CRP and CK in blood serum by enzyme-linked immunosorbent assay (ELIZA). The result showed that the serum concentrations of CRP and CK in cows suffering from retained placenta was 28.03 μg/ml and 238.93 ng/ml significantly higher than in cows with normal parturition 22.80 µg/ml and 137.50 ng/ml. The result of ELIZA showed that the serum concentration of CRP and CK in cows did not significantly affect depending upon the sex of the fetus and previous parturition history. On other hands, the serum concentration of CRP and CK in cows significantly differed depending upon the time to expel the placenta. In conclusion, the serum concentration of CRP and CK in cows with retained placenta was markedly higher than recorded in cows with normal parturition, and this can be used to identify days past since cows with retained placenta and to give a prognosis for their health and reproductive status.

Effect of lecirelin acetate, hCG or progesterone administration on day 7 post-insemination on conception rate and progesterone concentration in cross-bred cattle

M. S. Akhtar; S. Saleem; T. Ahmad; E. Ahmad; M. H. Lashari; M. M. Ayaz; L. A. Lodhi; I. Ahmad; I. Hussain; M. A khtar

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 2, Pages 149-153
DOI: 10.33899/ijvs.2019.153842

The present study was designed to evaluate the effect of administration of lecirelin acetate, hCG and progesterone after AI on ovarian picture, serum progesterone concentrations and first service conception rate in cross-bred cattle. A total of 160 lactating cross bred (Friesian x Sahiwal) cattle were divided into 4 groups after AI. The groups were treated as follows: control (injected i.m with normal saline 2ml, n=40), d 7-LA (injected i.m with lecirelin acetate100 µg, n=40), d 7-hCG (injected with hCG 3300 IU, n=40) and d 7-P4 (injected i.m with Progesterone 0.5 mgdaily for 4 days, n=40) group. The hormonal treatments were given to animals on day 7 after AI. The ultrasonography and blood sampling was done before treatment and then 7 days later. All animals were examined for pregnancy through ultrasonography at 40 ± 1 day after AI. The diameter of SCL on 7 days after treatment was higher (P < 0.01) in group d 7-LA and d 7-hCG group cows as compared to control cows. In contrast, the diameter of SCL in d 7- P4 treated cows did not differ from control cows. Formation of ACL was observed only in 50 % & 80 % cows in d 7-LA and d 7-hCG group respectively. No ACL was observed in control and d 7- P4 cattle on day 7 after treatment. The P4 concentrations were significantly higher (P < 0.01) in d 7-hCG treated cattle as compared to control at 7 days after treatment. As compared to 10 percent First Service Conception Rate (FSCR) in controls, an increase (P < 0.05) of 50 percent and 40 percent was observed in each treatment group (d 7-hCG & d 7-LA), non-significant (P > 0.05) difference in FSCR was observed between control and d 7-P4 group. It was concluded that the use of hCG or LA, 7 days after AI is a beneficial tool to improve conception rate in cross-bred cattle whereas use of parental P4 post-breeding has no effect on SCL diameter and conception rate.

Assessment of alterations in some blood biochemical and mineral contents concentration before and during pregnancy period in Iraqi ewes of Salah-edin province

M. A. Ismaeel; A. H. Awad; N. N. Dhahir

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 2, Pages 161-165
DOI: 10.33899/ijvs.2019.153844

Thirty local Iraqi ewes reared in Tikrit – Salah-edin province from first the of October-2016 to the first of March-2017 were used in the current study to investigate the influence of pregnancy on levels of some biochemical and minerals contents in the blood serum. Blood samples were collected from ewes before and monthly during pregnancy (first, second, third, fourth and fifth month), serum separated and stored at 5 °C until samples analysis. All samples were analyzed by spectrophotometer with special kits for each parameter. Results demonstrate significant decrease (P ≤0.05) in concentration of total protein, cholesterol and glucose as pregnancy advanced, while there is no effect of pregnancy on the concentration of creatinine and magnesium, however, there is a significant increase (P ≤0.05) in calcium concentration as pregnancy proceed. In conclusion the pregnancy has clear influences on the concentration of total protein, cholesterol, glucose and calcium. The measurements of these parameters give best assessment for nutritional and health status of Iraqi ewes during pregnancy.

Diagnosis of pregnancy and pathological conditions in the genital system of small ruminants by ultrasonography

Barra D. Al-Wataar; Rania M. Abdullah; Yasmen A. Omer

Iraqi Journal of Veterinary Sciences, 2016, Volume 30, Issue 1, Pages 9-13
DOI: 10.33899/ijvs.2016.119165

The current study aimed to investigate the possibility of using ultrasonography through abdominal probe in recording percentages of pregnancy, twins and genital pathological condition which affect the uterus for cases admitted to veterinary hospital in Kirkuk province as well as regulated visits to breeding projects of small ruminant and college of agriculture and forestry / Kirkuk university for the period from 01/10/2015 to 01/04/2016, which included 281 of ewes and does. The result of current study showed that that ratio of pregnancy was 75.9% in ewes and in does 47.6% while in case of twins in ewes 19.0% and in does 38.1%., while the genital pathological cases like endometritis and mucometritis (after birth) ratio in ewes 2.5% and in does 9.5%, in cases of pyometra in ewes 1.3% and in does 0.0%, while cases of embryonic deaths ratio in ewes 1.3% and in does 4.8%. It was concluded from the current study that; the possibility of diagnosis the genital pathological cases which affect the uterus as well as the identification of the single and twin pregnancy with investigation of the viability of embryos in both ewes and does by using ultrasonography with abdominal probe.

Alteration of serum haptoglobin concentration in normal parturition and dystocia affected cows

D.M. Aziz; S.H. Mohammed

Iraqi Journal of Veterinary Sciences, 2016, Volume 30, Issue 1, Pages 19-22
DOI: 10.33899/ijvs.2016.116864

This study was designed to investigate the alteration in serum haptoglobin (Hp) concentration in dystocia affected cows in comparison to those having normal parturition. Thirty cows included in this study, seventeen with normal parturition and thirteen with difficult parturition (dystocia). Blood samples, from cows that having normal parturition, were collected every three days during the last two weeks of gestation, at calving, 3, 7, and 10 days postpartum. Blood samples from dystocia affected cows were collected at calving, 3, 7, and 10 days postpartum. Serum Hp concentration was determined using ELISA. The results gradual increase of Hp concentration prepartum from 190±70 mg/L at the 10th day before calving to 250±30 and 260±100 mg/L at 7th and 3rd day prepartum, respectively. At day of parturition, Hp concentration increased to 300±140 mg/L, to reach 330±150 mg/L at 3rd day postpartum. Hp concentration at 7th and 10th day postpartum declined to 230±90 and 220±160 mg/L, respectively. Serum Hp of dystocia affected cows was 360±240 mg/L at calving. At 3rd day postpartum, Hp increased to 660±220 mg/L. At 7th and 10th days postpartum, Hp concentration declined to 510±300 and 400±110 mg/L, respectively. No variation in Hp was observed between the cows giving twins and those giving single calve. There was no significant effect of dystocia causes on serum Hp at calving and at 3rd day postpartum. It be concluded that normal calving and dystocia elevated the serum Hp concentration, and insufficient dilatation of birth canal was the most effective cause of dystocia. Also comparison of serum Hp concentration at 3rd and 7th day postpartum can be used as an indicator for the development of complications.