Main Subjects : Pond's Fish Diseases

Potential of probiotics Bacillus subtilis to reduce ammonia levels, Vibrio sp abundance, and increased production performance of Seaworm (Nereis sp) under laboratory scale

Aditya Kamaruddin; Moch Nurhudah; Djumbuh Rukmono; Angga Wiradana

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 4, Pages 757-763
DOI: 10.33899/ijvs.2021.128408.1572

This study aims to determine the potential of Bacillus subtilis probiotic in reducing levels of ammonia, Vibrio sp, and increased production performance in seaworm cultivation (Nereis sp.) under laboratory scale. Observation of the performance of seaworms (Nereis sp.) was carried out every 10 days which included weight gain (gr), length (cm), total biomass (gr), total bacteria (CFU/mL), total Vibrio sp. (CFU/mL), and total Bacillus subtilis (CFU/mL). Water quality measurements include temperature, dissolved oxygen (DO), pH, ammonia, and total organic matter (TOM). Application of probiotics Bacillus subtilis has the potential to reduce ammonia concentration, increase growth, and reduce the abundance of Vibrio sp under laboratory-scale seaworm cultivation. P3 treatment (0.01 mL with a probiotic density of 106 CFU/mL) gave the best results by being able to reduce the ammonia concentration by 47.5%. In summary, the probiotic application using the bacteria Bacillus subtilis with different densities able to provide good results in supporting production performance, maintaining the abundance of Vibrio sp., and reducing ammonia concentration in seaworm cultivation. This is the first study to report the performance of seaworm production using probiotic agent, research is still needed to determine the digestive enzyme activity of seaworms given probiotics.

Pathological lesions of acute sodium chloride toxicity in common carp: Case report

Saevan S. Al-Mahmood; Asseel M. Rahawi; Hadil B. Al-Sabaawy; Ahmed M. Farhan

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 3, Pages 417-419
DOI: 10.33899/ijvs.2020.126917.1417

Sodium chloride (NaCl) have a major impact on fish farming, as it used to disinfect fish and their eggs, in contrast using NaCl in high dose can cause life threating toxicity with high mortalities in Cyprinus carpio. We aimed in current report to introduce for specialist in fish culturing and farmers a typical acute NaCl toxicity by presenting pathological lesions. The histopathological lesions composed from sever degenerative and necrotic changes in gill filaments with destruction in blood vessels and hyperplasia in secondary gill lamella. In conclusion, using NaCl in high uncalculated doses can cause significant pathological changes that lead to 100% mortalities in Cyprinus carpio.

Effect of some mycotoxin on growth performance and feed utilization of Nile tilapia (Oreochromis niloticus)

F. I. Magouz; M.S. Salem; M.A. Hashad

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 99-108
DOI: 10.33899/ijvs.2018.153830

The present study was conducted in the fish lab, Sakha Aquaculture Research Unit, Central laboratory for Aquaculture Research-Abbassa, during season 2015, Feeding experiment was conducted for 16 weeks to study the effect of dietary contamination with aflatoxin B1 (150 ppb, AFB1) with and without the dietary supplementation of (1gm/kg) of Liquorice (medicinal plants), (2g) of MTB-100 (chemicals) and (0.5g) of Gromin-plus (chemicals) on growth performance and feed utilization of Nile tilapia (Oreochromis niloticus) fingerlings. A group of 150 Nile Tilapia fingerlings (O. niloticus) with an average initial body weight 13.50 gm were randomly allotted into 5 treatments in 10 glasses aquaria (80x35x40cm). Each treatment was applied in two aquaria. Fresh tap water was stored in fiberglass tanks for 24h under aeration for dechlorination. Prior to the start of the experiment, the fishes were adapted to a basal commercial diet (control diet) containing 31% crude protein and consisted of herring fish meal, soybean meal, yellow corn, wheat bran, sunflower oil and vitamins and minerals mixture for two weeks. These ingredients were pressed by manufactured machine (pellets size 1mm), milled and toxin AFB1 was added at a concentration of 150 ppb except the control. Fish in all treatments were daily fed the experimental diets at level of 3% of the fish biomass. The feed amount was given at two times daily, six days a week for 16 weeks. Fish were weighed weekly and the feed amounts were adjusted on the basis of the new weight. The aflatoxic dite has adversely affected the growth performance and survival rate, feed utilization and carcass composition in fish and residues of AFB1 in the whole body of fish and indices organs and blood biochemistry of the experimented fish. Dietary MTB-100, Liquorice and Gromin-plus inclusion alleviated aflatoxiccosis semptoms by fish, since it improved all the above tested parameters of aflatoxicated fish. Generally, obtained results in the present study indicated that the additives all the above could be used as detoxifying agents for aflatoxins.

Effects of feeding frequency on common carp ( Cyprinus carpio L.) growth rate

S.A. Hama Amin; N.M. Abdulrahman; V.M. Ahmed; P.B. Ibrahim; R.R. Ismail; M.B. Ahmed; N.M. HamaGareeb

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 1-4
DOI: 10.33899/ijvs.2018.153786

In present study the effect of feeding frequency on growth performance, food efficiency and survival rate of common carp fingerlings were investigated. Three groups of common carp fingerlings (average weight 22.88±1 gm) designed with three feeding frequencies one meal a day (D1), two meals a day (D2) and three meals a day (D3) with three replicates of each treatment. Fish in experimental treatments were fed by 4% of body weight. Feeding frequencies had significant effect on growth performance (P<0.05). The final body weight and specific growth rate (SGR) were significantly higher in group D3 (P<0.05) in this comparison. Similar responses were observed for body weight increased (BWI) and daily growth rate (DGR) and the best BWI and DGR were obtained in D3 group, that showed significantly different to other groups (P<0.05). The best feed conversion ratio (FCR) was obtained from three daily feeding (D3), however there were no significant different between D2 and D3 (P>0.05). Also feeding frequencies had positive effect on fish survival rate (P<0.05). The best results in growth performance and survival rate were obtained by feeding threemeals a day (D3).