Main Subjects : Fish Diseases


Effect of microalgae Spirulina spp. as food additive on some biological and blood parameters of common carp Cyprinus carpio L.

Nasreen Muhialddin Abdulrahman; Hawkar Jaafar Hama Ameen; Shamal Rasul Hama; Bakhan Rafiq Hassan; Pola Jalal Nader

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 1, Pages 27-31
DOI: 10.33899/ijvs.2019.125527.1049

This study was carried out to study the effect of adding different levels of the microalgae Spirulina spp. in fish laboratory of Animal Sciences Department, College of Agricultural Sciences of Sulaimani University, Sulaimani, Iraq. A total of 72 common carp fingerling weight 45±2 gm used to detect the effect of three different levels of the algae Spirulina spp. The control treatment T1 with 0%., T2 with 3 gm /kg diet and T3 with 5 gm Spirulina /kg diet. Each treatment in three replicates in which six fingerlings common carp were stocked in each aquarium. Results indicated no significant differences at PSpirulina) in T3 was higher significantly at PSpirulina was higher significantly at PSpirulina to common carp diets in different levels leads to significant differences in differential WBC counts, T3 with 5 gm Spirulina /kg diet was higher in WBC counts. Lymphocytes were higher in each of control and T3. Monocytes were higher in control, granulocytes in T2 with 3 gm Spirulina /kg diet. Adding of Spirulina affected significantly in cholesterol as compared to the control. Triglyceride was higher in control and T2. T3 with 5 gm Spirulina /kg diet leads to higher and lower significantly in HDL and LDL respectively. T1 and T3 were higher significantly in spleenosomatic index. Inclusion of S. platensis in fish diet as a feed additive or as a partial replacer of the expensive fishmeal imposes significant promotions in fish growth, coloration, reproduction and flesh quality.

Histopathological changes in diseased and treated catfish (Clarias gariepinus) by ciprofloxacin and clove oil

S.S. Abd El-Salam; M.F. Ghaly; D.M. Baraka; S.H. Mahmoud; A.A. El-Makhzangy

Iraqi Journal of Veterinary Sciences, Volume 32, Issue 1, Pages 13-19
DOI: 10.33899/ijvs.2018.153788

Bacteria pathogens may cause a serious loss in aquaculture and also health hazards to humans. On the basis of biochemical characteristics and API-20E system, all isolates of pathogens from diseased fish were identified as Aeromonas spp, the most predominant bacterial species were A. sobria 46.7%, A. caviae 19.03%, A. jandaei 8.6 % and A. veronii 8.6 %. One hundred and twenty Nile catfish Clarias gariepinus were divided into four equal groups (1,2,3 and 4) were challenged with Aeromonas sobria which predominant isolates, Gp. (1) was challenged only, while Gps. (2, 3 and 4) were treated after challenged with ciprofloxacin, clove extract and ciprofloxacin and clove extract in combination, respectively. The antibacterial activity of antibiotic and clove extract in their therapeutic doses decrease the mortality rate as (20, 16 and 13%) respectively compared with non-medicated Gp. (1) which recorded mortality 83%. Internal signs of infection with Aeromonas sobria in African catfish (Clarias gariepinus) include congestion of the hepatic blood vessels, areas of coagulative necrosis in the liver and hydropic degeneration of renal tubules according to the histopathological examination. In-vivo the combination of ciprofloxacin and clove extract was the choice as alternative bacterial therapeutic agent in infected Clarias gariepinus with Aeromonas sobria. Moreover histopathological changes have been widely used as biomarkers in the evaluation of the health of fish exposed to contaminants.

Effects of feeding frequency on common carp ( Cyprinus carpio L.) growth rate

S.A. Hama Amin; N.M. Abdulrahman; V.M. Ahmed; P.B. Ibrahim; R.R. Ismail; M.B. Ahmed; N.M. HamaGareeb

Iraqi Journal of Veterinary Sciences, Volume 32, Issue 1, Pages 1-4
DOI: 10.33899/ijvs.2018.153786

In present study the effect of feeding frequency on growth performance, food efficiency and survival rate of common carp fingerlings were investigated. Three groups of common carp fingerlings (average weight 22.88±1 gm) designed with three feeding frequencies one meal a day (D1), two meals a day (D2) and three meals a day (D3) with three replicates of each treatment. Fish in experimental treatments were fed by 4% of body weight. Feeding frequencies had significant effect on growth performance (P<0.05). The final body weight and specific growth rate (SGR) were significantly higher in group D3 (P<0.05) in this comparison. Similar responses were observed for body weight increased (BWI) and daily growth rate (DGR) and the best BWI and DGR were obtained in D3 group, that showed significantly different to other groups (P<0.05). The best feed conversion ratio (FCR) was obtained from three daily feeding (D3), however there were no significant different between D2 and D3 (P>0.05). Also feeding frequencies had positive effect on fish survival rate (P<0.05). The best results in growth performance and survival rate were obtained by feeding threemeals a day (D3).

Effect of some mycotoxin on growth performance and feed utilization of Nile tilapia (Oreochromis niloticus)

F. I. Magouz; M.S. Salem; M.A. Hashad

Iraqi Journal of Veterinary Sciences, Volume 32, Issue 1, Pages 99-108
DOI: 10.33899/ijvs.2018.153830

The present study was conducted in the fish lab, Sakha Aquaculture Research Unit, Central laboratory for Aquaculture Research-Abbassa, during season 2015, Feeding experiment was conducted for 16 weeks to study the effect of dietary contamination with aflatoxin B1 (150 ppb, AFB1) with and without the dietary supplementation of (1gm/kg) of Liquorice (medicinal plants), (2g) of MTB-100 (chemicals) and (0.5g) of Gromin-plus (chemicals) on growth performance and feed utilization of Nile tilapia (Oreochromis niloticus) fingerlings. A group of 150 Nile Tilapia fingerlings (O. niloticus) with an average initial body weight 13.50 gm were randomly allotted into 5 treatments in 10 glasses aquaria (80x35x40cm). Each treatment was applied in two aquaria. Fresh tap water was stored in fiberglass tanks for 24h under aeration for dechlorination. Prior to the start of the experiment, the fishes were adapted to a basal commercial diet (control diet) containing 31% crude protein and consisted of herring fish meal, soybean meal, yellow corn, wheat bran, sunflower oil and vitamins and minerals mixture for two weeks. These ingredients were pressed by manufactured machine (pellets size 1mm), milled and toxin AFB1 was added at a concentration of 150 ppb except the control. Fish in all treatments were daily fed the experimental diets at level of 3% of the fish biomass. The feed amount was given at two times daily, six days a week for 16 weeks. Fish were weighed weekly and the feed amounts were adjusted on the basis of the new weight. The aflatoxic dite has adversely affected the growth performance and survival rate, feed utilization and carcass composition in fish and residues of AFB1 in the whole body of fish and indices organs and blood biochemistry of the experimented fish. Dietary MTB-100, Liquorice and Gromin-plus inclusion alleviated aflatoxiccosis semptoms by fish, since it improved all the above tested parameters of aflatoxicated fish. Generally, obtained results in the present study indicated that the additives all the above could be used as detoxifying agents for aflatoxins.