Keywords : Goat


Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk

Zahra K. Saeed; Basil A. Abbas; Rasha M. Othman

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 259-263
DOI: 10.33899/ijvs.2019.125896.1176

The aim of this study was to identify the genetic diversity of lactic acid bacteria (LAB) isolated from the local goat's milk. A total of 100 raw milk samples were collected from the different Basrah local markets. All the samples were cultured in the De man, Rogosa, and Sharpe (MRS) medium which enhances the growth of lactic acid bacteria. The result of the study showed that the only 64 lactic acid bacteria isolated gave the Gram-positive and catalase-negative were 64 (64%). All the suspected isolates were detected and identified by using polymerase chain reaction (PCR) targeting the 16S rRNA gene and DNA sequencing. The sequencing results showed that 9 strains belong to Lactococcus spp. and 6 strainsbelongto Lactobacillus spp. and all tested isolates had similarity over 99% with those recorded in the GenBank of The National Centre for Biotechnology.

Effect of season on embryo production in Iraqi local black goat

Abdulstar Majeed; Ihsaan H. Al-Timimi; Mudafer N. AL-Saigh

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 59-65
DOI: 10.33899/ijvs.2019.125514.1036

The aim of this study was to investigate the effect of season on embryo production in Iraqi local black goat. 599 female genital system of Iraqi local black goat were collected from AL-Fallujah abattoir, AL-Anbar province during the period from July 2010 to the 1st July 2011. The oocytes were collected via aspiration and slicing. Only good and fair quality oocytes were selected for in vitro maturation. Fresh semen were used from two Bucks of proven fertility for fertilization . TALP , MEM , and TCM-199 culture media were used of in-vitro maturation (IVM) and in-vitro fertilization (IFV). It has been shown that high recovery and maturation rate 87.3% and 45.6% respectively were observed at breeding season as compared with recovery rate 72.5% and maturation rate 35.2% respectively in non-breeding season. There was a significant difference (P˂0.05) in recovery rate and maturation rate between different season. It has been found that fertilization rate was 60.05% in breeding season. While it has 33.9% in non-breeding season. There was a significant difference in fertilization rate (P˂0.05) between breeding and non-breeding season. Blastocyst obtained during breeding season was 56.41% , while it was 43.24% in non-breeding season. There was a significant difference (P˂0.05) between different season. It was concluded that there is an effect of season on oocyte recovery.