Keywords : follicular fluid

Effect of steroid-free follicular fluid antiserum on reproductive endocrine profile at estrous and metestrus phases in female rats

Jabber A. Al-Sa’aidi; Areej H. Al-Charack

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 273-278
DOI: 10.33899/ijvs.2019.125925.1187

Inhibins are important ovarian hormones that control the action of the pituitary gonadotropins which regulate the ovarian cycle. The present experiment aims to study the role of steroid-free follicular fluid-antibodies (S-FBFF-ab) on the reproductive hormone levels at estrus and metestrus phases in cycling female rats. Follicular fluid was collected from mature ovarian follicles, centrifuged and treatment with activated charcoal. S-FBFF was isolated and used for immunization of adult male rabbits. After 5 injections, blood was withdrawn for separation of S-FBFF-ab. Eighty mature female rats were assigned into control and treatment groups (40 each). At late metestrus phase, females of the control were injected intra-peritoneally with 100 μL of physiological saline, whereas treated females were injected intra-peritoneally with 100 μL of S-FBFF-ab. At early estrus and early metestrus phases of each estrous cycle, serum concentrations of inhibin-B, follicle stimulating hormone (FSH), estradiol (E2), and prolactin (PRL) were estimated. The results revealed that S-FBFF-ab has inhibin immuno-neutralization effect, which caused elevation in the concentrations of serum FSH and E2, and significant decline of serum PRL and inhibin-B, at early estrus, whereas significant elevation of E2 and PRL, and significant decline of serum FSH, at early metestrus. Furthermore, the results of gene expression revealed a significant elevation of ovarian aromatase gene at both early estrus and early metestrus phases, whereas pituitary PRL gene showed significant decline at early estrus phase and significant elevation at early metestrus phase. In conclusion, passive immunization against S-FBFF could augment the reproductive efficiency through increase reproductive endocrine activity.

Reproductive hormones profile of Iraqi Awassi ewes immunized against synthetic inhibin-α subunit or steroid-free bovine follicular fluid

S.S.A. Kafaji; J.A.A. Al-Sa; aidi; K.K. Khudair

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 2, Pages 123-128
DOI: 10.33899/ijvs.2017.145609

The current study was conducted to investigate the impacts of active and passive immunization against synthetic inhibin-α subunit and steroid-free bovine follicular fluid, respectively, on reproductive hormones profile out of breeding season in Iraqi Awassi ewes. Follicular fluid was aspired from mature bovine follicles, treated with activated charcoal, used for immunization of male rabbits, and obtaining of SFBFF antiserum. Forty non-pregnant Awassi ewes were allocated into 4 groups (n = 10 each). At day 38 of experiment, ewes were treated with intra-vaginal sponge impregated with medroxyprogesterone acetate 60 mg for 12 days. Ewes were treated at 0, 28 and 50 days with 4, 2 and 2 ml of normal saline (control; C-ve), 400, 200 and 200 µg of ovalbumine (C+ve), 400, 200 and 200 µg of inhibin (SI group), and 4 ml of normal saline at 0 day, and 4ml and 2ml of SFBFF antiserum, at 28 and 50 days, respectively (AI group). Blood samples were collected at 24 and 48 hours before and after sponge withdrawal for assessment of FSH, LH, inhibin-B, Activin-A, E2 and P4. Before sponge withdrawal, FSH level increased in SI ewes, whereas only after sponge withdrawal, FSH, LH, activin-A and E2 levels increased in SI and AI ewes. Opposite results were shown of inhibin-B level. In conclusion, active or passive immunization against inhibin in Awassi ewes could augment reproductive functions out of breeding season in Iraqi Awassi ewes.