Keywords : Mosul

Isolation and identification of Circovirus in pigeon

Safwan Yousif Al-Baroodi; Mozahim yasen Al-Attar

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126706.1364

The purpose of this study is first trial to detect of pigeon circovirus, so 1sr group include 100 cloacal swabs were collected 55 healthy and 45 ill pigeons, 36 yearlings and 64 adults, the 2nd group included organs was liver, spleen, bursa of Fabricius from 41 young pigeons 10-30 days old and bursa of Fabricius, liver, spleen from 28 dead in shell pigeon embryo in the 3rd group. DNA extracted from this samples and detection of virus DNA was attempt using polymerase chain reaction, after DNA amplification, the final products of the amplicon with 331 bp was cleared by using electrophoresis using agarose gel at concentration 2%. Results of viral DNA amplification were positive, which revealed as band in 331 bp the results showed that ill yearling pigeons recording high infectivity rate (66.7%)compare with healthy yearling pigeons and adult once, the bursa of Fabricius samples of dead yearling pigeons recorded high prevalence (36.58%) when compare with liver and spleen samples, DNA of pigeon circovirus high detected (60.71%) in bursa of Fabricius of dead in shell pigeon embryo. inconclusion pigeon circovirus affected the racing pigeon in Mosul, Iraq.

Molecular detection of Hammondia heydorni in dogs in Mosul city

Wasan A. Alobaidii

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 329-332
DOI: 10.33899/ijvs.2019.126052.1219

Through this study 122 individual fecal samples were collected from dogs, with different ages, sexes, and breeding management (stray and pet house hold). Some dogs were with healthy appearances while others were suffered from diarrhea, anorexia, depression, dehydration. Infection was investigated by light microscope after flotation technique by using Sheather's sugar solution. if the sample gives positive to oocyst (H. heydorni or other relative group), were mixed with potassium dichromate solution a rate of 2.5% for the stimulation of sporulation. DNA extraction was done using 2% sarcosyl, pronase E followed by phenol/chloroform extraction then precipitation attempt using ethanol. DNA amplification was attempt using H. heydorni primers JS4 and JS5 n microscope examination give 78 sample positives to the presence of oocyst, while polymerase chain reaction showed total percentage of infection with H. heydorni was 34.6% (27 case give positive reaction to PCR). Dogs less than 6 months of age show high infective rate 17.9%, dogs which suffer from gastrointestinal tract troubles (diarrhea, anorexia and dehydration) gave high infective rate 24.4%. Stray dogs gave high prevalence of infection to H. heydorni 25.6% when compared with pet house hold dogs which recorded 9% percentage of infection. This study is the first recorded H. heydorni in dogs in Mosul city.