Keywords : ELISA


Aflatoxin B1 residues in imported and local broiler,s breast and thigh muscle in Kurdistan region

A.M. Shareef; S.N. Taheer; M.Y. Yalda; I.Q. Sito; E.P. Candlan

Iraqi Journal of Veterinary Sciences, 2015, Volume 29, Issue 1, Pages 0-0
DOI: 10.33899/ijvs.2015.116854

Residues of Aflatoxins and their metabolites might be present in meat and other products of animals receiving Aflatoxin
contaminated feeds which could subsequently create health problems in man. Eighty nine imported (Iran/Khosh pokht);
(Turkey/Yam-tapilic, Lades, Senplic, Kapidac, Kozoa, Oznesilpilic) and (Brazil, hilal, Sadia), and 90 locally produced
(Hoshiar poultry farm, Nihad poultry farm, Hokar poultry farm, Mansoor poultry farm, AL-Shimal poultry house, Mardin
poultry house and AL-Eetimad poultry slaughterhouse) broiler breast and thigh muscle samples were examined for residual
Aflatoxin B1 using ELIZA test. Results revealed that out of 89 imported samples only 21 (23.59%) were positive, but only 2
(2.24%) were rejected, while the remaining 87 samples (97.75%) were acceptable. Concerning the local samples, results
showed that 19 samples (21.11%) were positive, but 10 (11.11%) were rejected, while the remaining 80 samples (88.88%)
were accepted. The public health importance of residual AFB1 in broiler meat samples was discussed.

Detection of streptomycin residues in local meat of bovine and ovine

O. A. Abdullah; A. M. Shareef; O. H. Sheet

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue 1, Pages 43-46
DOI: 10.33899/ijvs.2012.46958

From meat retails in Mosul province, forty-five meat samples of local ovine and bovine (23 bovine samples and 22 ovine samples) were collected. The period of collection was during November 2010 to May 2011, by means of multistage random sampling for detection of streptomycin residues. Enzyme linked immunosorbent assay (ELISA) was used for detection of streptomycin residues. The results revealed that eleven ovine meat samples (50%) were positive to streptomycin residue, with a mean value 35.06 µg kg-1, while 14 bovine meat samples (60.86%) were positive to residual streptomycin with a mean value 59.56 µg kg-1. From the results, it is clear that all tested meat samples (ovine and bovine) were safe enough for human consumption.

Use of Enzyme Linked Immunosorbent Assay for detection of aflatoxin M1 in milk powder

S. D. Al-Sawaf; O. A. Abdullah; O. H. Sheet

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue 1, Pages 39-42
DOI: 10.33899/ijvs.2012.46952

Thirty five samples were collected from seven types of milk powder in Mosul city markets, and surveyed for the presence of aflatoxin M1 (AFM1), by using Enzyme Linked Immunosorbent Assay (ELISA) technique. Analytical results showed that 82.8% of the samples were contaminated with AFM1. The incidence of AFM1 in Al-mudhish, Angolac, Dielac, Lona, Nido, Melgro and Multi samples were 40, 50, 80, 100, 100, 100 and 100%, respectively. The occurrence of AFM1 in milk powder, 79.3% (23 out of 29) of positive samples were higher than the permissible limits, according to the European Commission (50 ng/kg), whereas 6.8% (2 out of 29) of positive samples were above the prescribed limit of US regulation (500 ng/kg). The level of AFM1 concentration in Melgro and Multi types was higher than Al-mudhish type and it had a low level of contamination, compared with other types of milk powder.