Keywords : Histochemistry


Histomorphological and carbohydrate histochemical study of the pancreas in native ducks (Anas Platyrhynchos)

Saffanah K. Mahmood; Naziha S. Ahmed; Ghada A. Sultan; Mowaffak J. Yousif

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 4, Pages 1103-1110
DOI: 10.33899/ijvs.2022.133156.2183

In the current study, the histomorphological and histochemical characteristics of the pancreas in native ducks are examined with a particular emphasis on the structure of pancreatic acini and Langerhans islets (LI). Five adult male native ducks (Anas Platyrhynchos) are examined from the local market of Mosul. All samples are processed routinely for histological and histochemical analyses. Hematoxylin & Eosin, PAS-AB (pH 2.5), Toluidine blue, and Masson's Trichrome stains are used. The morphological examination reveals that male ducks’ pancreas is made up of three major lobes (dorsal, ventral, and third lobes). The pancreas is a small, semi-wide structure with a pale to white pinkish tone located between the duodenum branches. The findings show that the pancreas is enveloped in a thin layer of loose connective tissue capsule and mesothelial; septa extend from the capsule into parenchyma dividing it into many lobules. The study concludes that the pancreatic lobules are composed of a large area of exocrine acini and a small amount of LI, with an exocrine component consisting of round or oval acini with zymogen granules. The endocrine component is represented by three distinct LI types and lacks defined boundaries. Alpha islets are formed of alpha cells (ACs) and a few delta cells (DCs); beta islets are composed of peripherally located beta cells (BCs) and a few (DCs); and mixed islets are composed of alpha, beta, and delta cells. The present study sheds light on the morphological, histological, and histochemical characteristics of the indigenous duck pancreas. However, future research should focus on the pancreas of other bird species as well.

Histomorphometrical and histochemical study of the pancreas on the local dogs (Canis lupus familiaris)

Samyan L. Mostafa; Yahya Ahmed Mohammed

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 4, Pages 913-922
DOI: 10.33899/ijvs.2022.132567.2105

In the current study, ten adult healthy local dogs of both sexes were used to perform histomorphometrical study on their pancreases. The dog pancreas had an inverted V-shape consisting of left and right lobes joined by a body. The pancreas possesses both exocrine and endocrine parts. The exocrine portion was composed of numerous acini and fewer tubules as well as the duct system. The number, total diameter and cellular height of acini were significantly abundant, larger and taller in the right lobe compared those of the body and left lobe. Furthermore, the number and total diameter of large pancreatic islets were significantly abundant and larger in the left lobe despite with those of the body and right lobe. This result leads to consider the right lobe was a target region for enzymatic secretion, while the left lobe has a specific function for hormonal secretion. According to available literature, no such result and thus conclusion had been reported on dog pancreas previously. In all parts of the pancreas and with aldehyde fuchsin stain, only the large pancreatic islets contained α-, β-, δ-cells as well as non-staining cells. Only, the β-cells occurred as single cells between the pancreatic acini or even within the connective tissue septa. These single cells were more numerous in the left lobe, but they were infrequently located in the body and right lobe.

Structural study of uterine tubes of the rabbit (Oryctolagus cuniculus) at different postnatal periods

Faeq Jabar Al-Saffar; Massarat Almayahi

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 277-288
DOI: 10.33899/ijvs.2019.162911

This study was designed to investigate the histomorphological changes in the uterine tubes of the local rabbits during three different periods of ages that were kitten, immature and mature stages of life. For such study, twenty-four female rabbits were used. Procedure of routine histological technique was performed to prepare histological sections by using general and special histochemical stains. Gross findings revealed the presence of bilateral uterine tubes which comprised thick short coiled preampulla, thin long ampulla and the isthmus which was shortest and straight region joined to the uterus. Microscopically three regions were identified according to the differences in their histological characteristic features. Data revealed that the wall of uterine tubes in kittens was not fully developed, whereas in immature does, global histological and histochemical changes were identified compared to kittens while mature does indicated critical role of the post weaning period on the development of the animals. Changes were specially recognized by histochemical procedure which showed strong positive reactions especially isthmus which obviously reacted with PAS, Ab, combined PAS-AB. In conclusion, current data showed unique differences of the uterine tubes in the local rabbits compared to other mammals especially those of rodentia species.