Keywords : Brucellosis

A serological study of brucellosis in camels south of Kirkuk, Iraq

M. Yawoz; S. E. Jaafar; A. I. Salih; M. H. Abdullah

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue 2, Pages 105-107
DOI: 10.33899/ijvs.2012.67484

A study of the prevalence of antibodies to camel brucellosis has been carried out in the south of Kirkuk city during March 2011. A total of 66 camels (6 male, 60 female) involved in this study with age between 6 months to 22 years. Blood samples were collected from jugular vein and the sera samples were screened by using Rose Bengal Plate Test (RBPT). The sera that were positive by using RBPT have been tested again by using the 2-Mercaptoethanol (2ME) test. The results of this study revealed that two camels from 66 camels (3.03%) were seropositive for brucella antibodies in South of Kirkuk city.

Antioxidant status in pregnant ewes vaccinated with Rev 1 against brucellosis

W. S. Al- Khafaji; M. I. Al-Farwachi

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue 1, Pages 15-19
DOI: 10.33899/ijvs.2012.46890

The aim of this study was to examine the changes in the indicators of free radicals and antioxidant activity, represented by malondialdehyde and glutathione peroxidase in the sera of ewes vaccinated with Rev 1 vaccine. The experiment included 28 animals which were divided into four equal groups. Animals of the first and second groups were vaccinated subcutaneously with 2×109 and 2×107 colony forming units (CFU), respectively, whereas the animals of third and fourth groups were vaccinated conjunctively with 2×109 and 2×107 CFU, respectively. Sera were collected monthly for 6 months. Antibody responses were assessed by classical tests (Rose Bengal test, tube agglutination and 2-mercaptoethanol tests) in comparison with competitive ELISA. The antibody titers were higher and remained for along period in the subcutaneously vaccinated groups with the two doses compared those vaccinated conjunctively. There was a significant increase in serum glutathione peroxidase activity in the 8th week post vaccination in subcutaneously vaccinated groups and during the 12th week in those vaccinated conjunctively. Significant increase of serum malondialdehyde levels occurred during the 4th week in those vaccinated conjunctively and in 8th week in those vaccinated subcutaneously. This study concluded that the route of administration of the vaccine affects glutathione peroxidase activity and malondialdehyde level, which act as indicators of oxidative stress response, more than the vaccine dose.