Keywords : Escherichia coli

The effect of thyme (Thymus daenensis) supplement on growth and hygienic parameters of broilers meat

Majid Gholami Ahangaran; Niloofar Peimani; Asiye Ahmadi Dastgerdi

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 87-92
DOI: 10.33899/ijvs.2019.125526.1048

The antibacterial effect of Thyme species was relatively approved but the effect of this plant on bacterial population of intestine in chickens and consequently on bacterial load of chicken meat following administration of thyme was not investigated. For evaluation of thyme effect on growth indices, microbial contamination of meat and microbial population of cecal content, 180 broiler chicks were divided into 4 equal groups with 3 replicates and were reared until 42 days. Chickens in different groups were treated with different doses of thyme (0, 0.25, 0.50 and 1 percent) in diets. The weight gain, feed consumption and feed conversion rate were calculated weekly. In the end of 42 days old, all chickens were slaughtered. The population of Escherichia coli per gram of cecal contents and contamination of meat with Escherichia coli were assayed. The data showed that supplement of 0. 5 and 1 percent of thyme to diet elevated weight gain and decreased feed conversion rate. Furthermore, addition of 0.5 and 1 percent of thyme to diet can decrease microbial population of cecal content and meat. In conclusion, the addition of thyme to diet may improve growth rate and hygienic parameters in chicken meat.

Detection of Escherichia coli hlyA gene and Staphylococcus aureus Sea gene in raw milk of buffaloes using RT-PCR technique in AL- Qadisiyah province

J.N. Sadeq; Kh.H. Fahed; H.J. Hassan

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 87-91
DOI: 10.33899/ijvs.2018.153815

The aim of this study was to determines the prevalence of virulence gene hemolysin A (hly A) Escherichia coli and Staphylococcal enterotoxins (sea) in Staphylococcus aureus in raw milk buffaloes. In molecular laboratory, real-time polymerase chain reaction (RT-PCR) technique has been performed for 24 samples which have been taken randomly from Buffaloes milk, using primers of high specificity for Escherichia coli hlyA gene and Staphylococcus aureus Sea genes. The results showed different degrees of the studied genes activities. Four out of 24 samples represented S. aureus Sea gene (16.6%) whereas 16 out of 24 samples represented E. coli hlyA gene (66.6%). this study concluded that buffaloes milk might be a source of contamination with pathogenic bacteria of virulent genes which may have different levels of activities.