Keywords : Polymorphism

Detection of similarity and genetic distance between Iraqi chicken varieties and different standard strains

Thamer A. Ezzulddin; Dhyaa M. Jwher; Sufian A. Dabdoub

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 333-337
DOI: 10.33899/ijvs.2019.126109.1235

Forty-eight wing vein blood samples were collected from different locations of poultry rearing farms and back yard chickens of Nineveh governorate from the of local and exotic chicken. The chicken divided into twelve groups four birds each according to colors and phenotype for the local and exotic chicken respectively. Blood DNA was extracted and amplified by thermocycler apparatus and the electrophoresis was done using 1.2% agarose gel for DNA bands exhibiting. The results showed high genetic similarity within the local chickens ranged between 0.78- 0.96 at an average of 0.88, while it ranged between 0.73- 0.86 at an average of 0.78 in exotic breeds. The degree of similarity between Iraqi and exotic breeds was 0.74-0.88 at average of 0.80. The calculated average of differences among each of Iraqi and exotic chickens and in between were 0.12, 0.22 and 0.20, respectively. However, the genetic distance within the local chicken, exotic breed and in between them was 0.128, 0.24 and 0.21 respectively. The study concluded that the genetic similarity was higher within local chicken groups than those of exotic breeds.

Molecular characterization of heat shock protein 70 gene in Iraqi buffalo

Hassan N. Habib

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 139-143
DOI: 10.33899/ijvs.2019.125633.1116

The heat shock protein 70 (HSP 70) has important roles in protecting cells and keeping them alive when exposed to different stress conditions. The polymorphism of the hsp 70 gene could be linked with the ability of stress tolerance. This study aimed to determine the polymorphism of the hsp 70 gene in Iraqi buffaloes and study bits effects on the resistance to stress. This study was conducted during from November 2018 to February 2019. The number of buffalo females used was 35 at the age of 4 - 6 years, which belonged to the local farmers from Basra city, Iraq. The DNA was extraction from the blood samples then the polymerase chain reaction (PCR) amplification was performed. The DNA sequences were analyzed by using bioinformatics analysis. The results of the molecular analysis showed that there were two groups of the hsp 70 gene as a compare with the same genes in GenBank due to silent and missense mutations. Based on these findings, it can be concluded that the Iraqi buffaloes have adapted to the surrounding environmental conditions as a result of the action of HSP 70 proliferation. The hsp 70 gene was a useful biomarker of stress tolerance in buffaloes.

Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen as a reference for genetic selection

B. Utomo; E. Safitri

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 113-118
DOI: 10.33899/ijvs.2018.153832

Research on genetic polymorphism of growth hormone (GH) and receptor growth hormone (rGH) has not been done in crossbred of Limousin cattle, so it is interesting to be examined. Blood samples were taken from 14 Madura calves were artificially inseminated with Limousin cement. DNA amplification is done by using Polymerase Chain Reaction (PCR) method, Restriction Fragment Length Polymorphism (RFLP) method to determine the genotype. DNA sequencing was done to determine nucleotide sequences of GH unit genes. The results showed that identification of GH and rGH gene polymorphisms was done by breaking DNA fragments from 432 and 298 bp in Madura and Limousin cattle (Madrasin) ie, L and V alleles have a frequency of 0.67 and 0.33 for the GH gene, respectively. This proves that the crossed-breeding of Madrasin have V allele that is not owned by the Madura cattle. While in the rGH gene, the A allele is 0.92 and the G allele is 0.08, with the frequency of the A allele larger than the G allele. This research concluded: that GH and rGH undergo changes on polymorphisms in Madrasin cattle can be used as a basis for selection.

Influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition in beef calves

T.A. Sedykh; L.A. Kalashnikova; I.V. Gusev; I.Yu. Pavlova; R.S. Gizatullin; I.Yu. Dolmatova

Iraqi Journal of Veterinary Sciences, 2016, Volume 30, Issue 2, Pages 41-48
DOI: 10.33899/ijvs.2016.121382

The authors investigated the influence of TG5 and LEP gene polymorphism on quantitative and qualitative meat composition of 20 month old bull calves of the Hereford (n=38) and Limousine (n=26) breeds, which were bred in the climate of Cis-Ural steppe zone from 2013 to 2015. The Hereford calves were the offspring of the cattle from the southeastern states of Australia and Tasmania (3rd descent of the main lines: Baz-Gol-Sol 2U 6827, Domino 325676, Ardmors-Domino 56, Silverlend 31432); the Limousine calves were the descendants of the offspring resulting from the accumulation cross breeding of Simmental cattle with the French Reproductive Recognized bulls (4th descent of the Reper 433 and other lines). The analysis of TG5 genotype frequency in the examined populations reveals that the animals have significant (P<0.01 or P<0.05) potential for increased taste and nutritional qualities of beef associated with a high proportion of desirable TT genotype, probably related to the foreign origin of the cattle. There were no carriers of the BB genotype of LEP genes in the examined populations. Significant (P<0.01 or P<0.05) dependence between the studied SNP in TG5 and the rates of total body fat, the proportion of adipose tissue in the morphological carcass composition (or meat composition), and the content of intramuscular fat in the longissimus, as well as the correlation between the studied SNP in LEP and the rates of raw visceral fat, and fat outcome, were established. LEP polymorphism was significantly (P<0.01 or P<0.05) associated with the proportion of adipose tissue in the morphological carcass composition in Hereford calves, and with the content of intramuscular fat in the longissimus in Limousine calves. The results of our study of TG5 and LEP polymorphism demonstrate considerable genetic potential of the given populations of the Hereford and Limousine breeds in relation to the quantitative and qualitative composition of their meat; they are to be used in improvement of genetic potential of meat cattle in the Cis-Ural steppe zone.