Keywords : Rat

The effect of CO-Q10 on the testicular histological changes in rats induced by imatinib

Luma K. Al-Allaf; Hafidh A. Al-Ashoo

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126587.1347

The safety zone of imatinib, and specifically its relevancy to organ toxicity, has been discussed dialectically in current years. Oxidative stress may be one of the causes of imatinib -mediated toxicity. This study aimed to examine the possible role of co-enzyme Q10 in ameliorating the adverse effect of imatinib on the testicular histology of male albino rats -if it is present. Twenty-eight male Albino rats were used randomly assigned to 4 experimental groups: Group 1 includes 40-45 days aged rats (n=8) which were gavage a dose of 200 mg/kg/day/30 days of imatinib mesylate. Group 2 includes age matched control rats which were administered with distilled water(n=4). Group 3 includes eight rats were received Q10, 50mg/kg, alone, Q10-sorb capsule of 50 mg given with the same schedule. Group 4. Includes eight rats were co administrated orally with 50 mg/kg of Q10+200 mg/kg of imatinib (once/day/30days). Euthanizing of animals with ether 24 hours after the final dose was done. Testes of rats from each experimental group were obtained. The tissues processed and stained by routine histological method. Histological sections of testes's rats treated with 200mg/Kg of imatinib revealed different testicular lesions compared to those of control group (P<0.05). Six 6(75%) of these sections revealed degenerated tubules, detached Sertoli cells, and apoptosis. These histological sections also showed thick tunica albuginea, seminiferous tubules with thick basement membrane. sometimes only a few of Sertoli cells were appeared in histological sections of imatinib treated rats. Mean Johnsen’s scores in these sections was 5.1±0.1 (P˂0.001). Features of retained spermatid were also noticed in some sections. There was significant reduction in both seminiferous tubular diameter and the epithelial height of histological sections of group 2(P˂0.001) with mean of 140.2±3.2µm and 14.8±1.1 µm respectively. Moreover, the number of Sertoli cells/ seminiferous tubule were significantly increased (P˂0.001), with mean of 27.4±0.2 and Leydig cell number is also significantly raised with mean of 7.5±0.5. In conclusion, treatment of peripubertal rats with imatinib induced several testicular alterations (including Sertoli cells) in comparison to control rats indicated that this drug is a gonadotoxic agent as it affects the quality and quantity of spermatogenesis. An Ameliorating effect of co-enzyme Q10 co-administration on imatinib-induced testicular toxicity was concluded.

Biochemical and histopathological study of thioredoxin reductase isolation from blood serum in normal and oxidative stress-exposed rats

A.A. Hamdon; L.A. Al-Helaly

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 115-124
DOI: 10.33899/ijvs.2019.163243

The study included investigation of effects of the thioredoxin reductase isolated from serum of human on oxidative stress induced in rats, through histopathological examination of the heart and liver, and the measurement of the biochemical parameters, which included: thioredoxin reductase, creatine kinase, alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin and uric acid. Treating experimental animals with 1% hydrogen peroxide led to a significant increase in thioredoxin reductase, creatine kinase, aspartate aminotransferase and total bilirubin compared to control group, while a significant decrease in: albumin and uric acid, but non-significant in alanine aminotransferase. As well as, a different dose 2 and 4mgl Kg of rat body weight of isolated TrxR with 1% hydrogen peroxide improved parameter levels through decrease oxidative stress that induced in rats. The results of the histopathological examination revealed slight to moderate changes in the heart, while no distinguishing changes were observed in the heart of the group treated with hydrogen peroxide and injected with enzyme in intraperitoneally with 4 mg/ kg of rat weight compared to control group. In the liver, there was observed vascular degeneration and thickening of hepatic capsule as a result of chronic congestion and degeneration in the blood vessels which after treatment with 1% hydrogen peroxide compared to control group, but there was noticeable improvement in the liver of group treated with hydrogen peroxide and injected with the enzyme in intraperitoneally with 4 mg/kg of rat weight, and these results confirm the role of the enzyme in the protection of the body from oxidative stress, the use of the enzyme can reduce the severity of different diseases.

Trypanocidal efficacy of diminazene in diabetic rats

U. S. Chigozie; A. B. Maduka; J. G. Ifeanyi

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue 1, Pages 33-38
DOI: 10.33899/ijvs.2012.46950

The experiment was conducted to investigate the impact of hyperglycaemia on the trypanocidal efficacy of diminazene aceturate. Groups of alloxan-induced diabetic rats infected with T. brucei and T. congolense were treated with diminazene aceturate, and trypanocidal effects compared with normal non-diabetic controls. Results showed that the prepatent period was shorter in the diabetic (11.25±1.65 days) than non-diabetic-T. congolense (15.0±1.73 days), and also variations in responses to the trypanocidal therapy between the diabetic and non-diabetic groups were detected. Parasite clearance time did not differ significantly between the diabetic and non-diabetic (43.2±8.89 versus 52.8±8.89 hours in T. brucei and 33.6±5.9 versus 36.0±6.93 hours in T. congolense, respectively). The relapse intervals were shorter in the diabetic than non-diabetic (16 days versus 23 days in T. brucei, and 7 days versus 14 days in T. congolense, respectively). Proportion of relapses was greater in the diabetic- (100%) than non-diabetic-T. congolense (66.7%). We also find parasite species-related differences in susceptibility to the trypanocide, with a higher apparent cure rate in the T. brucei than T. congolense group. We conclude from the results of this study that the chemotherapeutic effectiveness of diminazene aceturate may be diminished in patients with diabetes mellitus. Further study is needed to validate this hypothesis.