Keywords : Cattle


Phylogenetic tree analysis study of bovine papillomaviruses type 1 based on L1 gene in Al-Qadisiyah governorate, Iraq

Khalefa Ali Mansour; Hassan Hachim Naser; Muthanna Hadi Hussain

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 1, Pages 151-155
DOI: 10.33899/ijvs.2019.125535.1057

Bovine fibropapilloma and papilloma occur in different parts of the skin of animals. Bovine Papillomavirus (BPV) is an oncogenic virus making benign tumor lesion of together mucosal and cutaneous tissue in cattle. In order to confirm the clinical diagnosis; the study planned to make the molecular detection of BPV (DNA) using Polymerase Chain Reaction (PCR) from skin lesions and the phylogenetic analysis. Thirty-eight samples of skin lesions were collected from cattle clinically suspected to be infected with bovine papilloma virus from herds in Al-Qadisiyah Governorate in 2016, the primary clinical diagnosis depended on the morphological appearance and features of the lesion. Deoxyribonucleic Acid (DNA) was extracted from skin lesions; the DNA was examined by PCR technique using specific primer to BPV-1 /L gene-1. Twenty-two samples out of 38 (57,9%), which were collected from different regions in Al-Qadisiyah Governorate, were positive. The sequences of four positive samples of DNA product amplification of (BPV) type-1, L1 gene confirmed the PCR results. These samples had the DNA presented in four accession numbers KY662042-1, KY662043-1, KY662040-1 and KY662041-1. This study proofed that cutaneous bovine papillomatosis related with BPV-1 infection in the cattle herds has affinity to solid skin rather than other epithelial and mucosal tissue.

Characterizing physiological status in three breeds of bulls reared under ecological and climate conditions of the Altai region

L.V. Osadchuk; M.A. Kleschev; O.I. Sebezhko; O.S. Korotkevich; N.I. Shishin; T.V. Konovalova; K.N. Narozhnykh; V.L. Petukhov

Iraqi Journal of Veterinary Sciences, Volume 31, Issue 1, Pages 35-42
DOI: 10.33899/ijvs.2017.126708

Ecological and climatic factors have an impact on the health, productivity and reproduction of the cattle. The goal of this work is the study of physiological status of servicing bulls reared under ecological and climate conditions of the Altai region, by defining the differences between Red-Steppe, Simmental and Black-and-White breeds in spermatogenic, hormonal, biochemical and hematological parameters. Samples of peripheral blood and ejaculates were taken from 48 grown-up servicing bulls (average age 5.6±0.3 years) in autumn period. It was established that Red-Steppe bulls have higher concentrations of nonorganic phosphorus, leucocytes, erythrocytes, hemoglobin, hematocrit and erythrocyte sedimentation rate in comparison with animals of Simmental breed, and the hemoglobin concentration and hematocrit are also higher in comparison with Black-and-White breed. It was noted that bulls of Black-and-White breed have a higher level of cortisol in comparison with the other breeds. The lowest level of serum urea and total protein and increased serum activity of creatine kinase and γ-glutamyl transferase, as well as the heaviest body weight were observed in Simmental breed bulls. The differences between breeds in a wide spectrum of physiological measures reflect not only genetically determined peculiarities of homeostatic mechanism, but also may reflect different ability to adapt to local ecological and climate conditions of the Altai region. Spermatogenic, biochemical and hematological measures in bull sires reared in the Altai region were similar to those in bulls bred in other Russian regions and some other countries. The measures reported could serve as reference values and therefore represent ‘normal’ values of physiological status for these bull sires reared in this ecological and climatic zone, but could be utilized in further studies for comprehensive monitoring of cattle breeding stock in other ecological and climatic zones of the Siberian region of Russia.

The first unequivocal report of Encephalitozoon cuniculi in rabbits and Encephalitozoon intestinalis in cattle in Mosul city-Iraq

Saevan Saad Al-Mahmood; Hafidh I. Al-Sadi

Iraqi Journal of Veterinary Sciences, Volume 29, Issue 1, Pages 0-0
DOI: 10.33899/ijvs.2015.116859

The aim of present study was to report spontaneous Encephalitozoon cuniculi (E. cuniculi) infection in rabbits and Encephalitozoon intestinalis (E. intestinalis) infection in cattle in Mosul city - Iraq. Fecal samples were collected randomly from 142 rabbits and 115 cattle during the period from June, 2012 to June, 2013. Thin smears were prepared from the fecal samples and stained with different fecal stains, as well as the indirect immunofluorescent antibody technique. Infected rabbits were necropsied and pathology of the infection was described. The result showed that microsporidiosis was detected in 41 (26.9%) rabbits and in 12 (10.4%) cattle. E. cuniculi spores were identified in 31 out of the 41 rabbits (73.2%) and E. intestinalis in 7 out of the 12 cattle (58.3%). Infected rabbits showed vasculitis, granulomatous encephalitis, chronic interstitial nephritis, granulomatous hepatitis, cholangitis, vasculitis and non – suppurative pneumonia, infiltration of mononuclear cells in most layers of the intestines, and hyperplasia of lymphoid tissues. E. cuniculi spores were seen in the liver, kidneys and lungs. This study considered the first one that report microsporidiosis (E. cuniculi, E. intestinalis) in Mosul city - Iraq.

Clinical, epidemiological and therapeutic studies on bovine tropical theileriosis in Faisalabad, Pakistan

M.I. Saleem; A. Tariq; A. Shazad; S.A. Mahfooz

Iraqi Journal of Veterinary Sciences, Volume 28, Issue 2, Pages 0-0
DOI: 10.33899/ijvs.2014.116957

While screening of selected herds of cattle (each having minimum 15 animals), 28 animals were found positive for tropical
theileriosis (Theileria annulata) from a total of 260. The overall bi-monthly incidence rate was 10.8% from April-July, 2011 in
Faisalabad. 89.3% and 10.71% in females and males respectively. To check breed wise prevalence, 4 out of all 28 positive
animals were of Red Sindhi and Dhanni showing 14.2% prevalence, and rests of all were crossbred with 85.71% prevalence.
The bi-monthly incidence during April-May was 28.57% while in June-July this was 71.42%. The suspected cattle were
subjected to analysis by making thin blood and lymph smears. After confirmation, 28 animals were divided into two equal
groups. The animals of group A were treated with extracts of Peganum harmala harmala at the dose rate of 7 mg/kg b.w
intramuscularly while those of group B were treated with buparvaqoune at-the dose rate of 2.5mg/kg b.w intramuscularly
(According to manufactured Comp.). Twelve animals were recovered after treatment with P. harmala (85.71%) and 2 could
not be cured and died (14.28%) while in case of animals treated with buparvaquone, 13 cattle recovered (92.85%) and one
animal died (7.14%). The obtained data were then subjected to Chi-Square Test for analysis of variance.

Comparative study of the auricle and external acoustic meatus of the cattle and buffalo

S. Al-Sadi; A. A. Hasso

Iraqi Journal of Veterinary Sciences, Volume 26, Issue 2, Pages 65-72
DOI: 10.33899/ijvs.2012.67456

The present work was design to study the anatomical features and radiographic appearance of auricle and external acoustic meatus of cattle and buffalo, collected from (12) sample of heads were used in this study, there were equally divided into three groups: first group to study the shape, position and relation of cartilage, muscles and ligament of auricle in both animals, second group to study the measurements of external acoustic meatus, and the third group study morphological and radiographical of external acoustic meatus in both animal, the study revealed that the auricle is a flapy flashy appendage attached to the side of the skull by muscles and ligaments, the auricle is funnel shape, distally is wide open, but more proximally, it is rolled up to form a tube that bend medially to be connected to the external acoustic meatus, the auricle in both animals is composed of three cartilage: the part auricular, scutiform and annular cartilage also auricular muscle and ligament which support the external ear. The result of this study shows that intrinsic auricular muscles is more developed in buffalo, while the greater parts of the extrinsic auricular muscles are developed in both animals. The present work shows that the frontoauricular muscles are distinguish into two parts in buffalo and cattle on the other hand the interscutularis muscle thin fibers and attached with scutuloauricularis prefunds muscle in buffalo, also the parotidauriculares narrower and thicker in cattle, but a ribbon -like muscle thin and wide in buffalo. The external acoustic meatus begins where the rolled up part of the annular cartilage narrows and ends, the meatus has cartilaginous and osseous parts it is lined with skin the study provided that acoustic meatus about 5.667±0.0816 cm of length in cattle and 6.500± 0.126 cm in buffalo, the aim of present work is to report more detailed information about the auricle and meatus in both animal for value importment can easily examined by the speculum the shortness of the meatus should be courses of the risk of injuring of the tympanic membrane and to be able to pass the otoscope tube through the external meatus and to recognize the eardrum when it is seen, to know the surgical anatomy of auricle and acoustic meatus and to know the relationship of vessels and nerve that must be avoid during surgery and to be able to recognize all parts of the temporal bone on radiographs.