This study aims to investigate the effect of aspirin in methotrexate toxicity (increase or decrease) relating to oxidative stress and histopathological changes of the liver and kidney in male rats. Twenty rats were divided into equal 4 groups, the first is considered control group, the second was treated with methotrexate in a dose of 10 mg/kg, the third was treated with methotrexate and aspirin in doses of 10 and 30 mg/kg respectively, the fourth was treated with aspirin alone with a dose of 30 mg/kg. All doses were given by daily oral dosage for 4 constitutive days. The result revealed a significant decrease in the concentration of both glutathione and albumin and a significant increase in the concentration of both malondialdehyde and ALT enzyme in the two groups treated with methotrexate alone or in combination with aspirin as compared to the control group. The histopathology revealed that the severity of lesions was in the group of methotrexate with aspirin, group of methotrexate only and a group of aspirin respectively, which are representing by coagulative necrosis and hypertrophy of hepatocytes in the liver while the lesions of kidney were atrophy of some glomeruli and renal cystic formation. The study concludes that aspirin increases the toxic effect of methotrexate at the level of oxidative stress concomitant with the occurrence of hepatic and renal toxicity.
This study includes collection of 120 samples of sheep esophagus from slaughtered sheep in Mosul abattoir from December 2013 to March 2014 for detection of lesions and their incidence. Total 85 esophagus samples showed lesions from the total collected samples. The revealed lesions included disturbance of cell metabolism manifested by coagulative necrosis, sloughing of epithelial lining the esophagus and vacuolar degeneration at incidence rate 67.7, 23.5 and 17.6% respectively, circulatory disturbances like petechial, diffuse hemorrhage and edema were noticed at incidence rate 7.05, 4.7 and 3.5% respectively. Also results showed epithelial hyperplasia, fibroplasia and hyperkeratosis at 47, 24.7 and 31.7% of the total collected samples respectively, whereas eosinophilic inflammation of esophagus appeared at 3.5% of collected samples. Jaundice represent the disturbance in pigmentation reported at ratio of 16.4%, also additionally there was parasitic infestation represented by sarcocystosis and worm infestation which impeded in muscularis layer of esophagus at percentage ratio 50.5% and 3.5% respectively and a single case of esophageal diverticulosis was recorded at percentage ratio of 1.17%. Section that stained which Masson trichrome stain showed fibroplasia with proliferation of fibroblasts that take a bluish green color between muscle fibers.
One hundred fifteen samples of the urinary bladder were collected randomly from slaughtered local bovine calves in Mosul city during March 2018. Gross and histopathological changes revealed in 37 samples 23.1%. Gross changes represented by congestions, petechial hemorrhage, inflammation and cystic hernia. Histopathological changes included the disturbances of cell metabolism represented by cell swelling of transitional epithelial cells and smooth muscle cell 16.2%, and coagulative necrosis of epithelium of mucosa and Zenker's necrosis of muscular layer 40.5%. cell adaptation manifested by epithelial hyperplasia 18.9% and smooth muscle fibers atrophy 8.1%. Circulatory disturbances represented by hyperemia and congestion 51.3%, petechial hemorrhage, edema and thrombi 5.4% for each. Acute cystitis showed in percentage of 18.9% while chronic cystitis was 13% and fibrosis 21.6%. Congenital deformities represented by cystic hernia 13% and bladder wall intrusion 5.4%. This study concludes the presence of different types of pathological changes in the urinary bladder of slaughtered local bovine calves in Mosul city which indicates presenting different types of causative agents and infections.