Keywords : Wound healing

Histological and antimicrobial effects of tramadol infiltration on incisional oral mucosal wound healing in rabbits

F.A. Al-Mashhadane; E.A. Mustafa; G.A. Taqa

Iraqi Journal of Veterinary Sciences, Volume 33, Issue 2, Pages 335-340
DOI: 10.33899/ijvs.2019.162996

The present study compared effects of tramadol with lidocaine on healing processes of incisional intraoral mucosal wounds in rabbits and to study the antimicrobial effect of tramadol. For this purpose, fifteen apparently healthy male rabbits of 5-6 months old were used. They were classified into three groups, first group treated with 0.5 ml distilled water infiltration as control (n=5), second group treated with 0.5 ml tramadol infiltration in dose of 100 mg/2 ml (n=5), and third group treated with 0.5 ml of 2% vasoconstrictor-free lidocaine infiltration, then an incision was done to the buccal mucosa of all animals and they slaughtered on day 10 and their wounds were evaluated by histopathologic for presence of edema, vascularity, and inflammatory cells. For antimicrobial effect, swabs from tramadol group were taken from sites of wounds before incision and other swabs were taken after surgical incision on the first day, third day and seventh day, then the colony forming units (CFU)/ml of microorganisms were counted. The present study found that the infiltration with tramadol in dose of 100mg/2ml result in new epithelialization and wound healing occurred faster than infiltration with lidocaine 2% group also this study found that tramadol has significant decrease in the means of colony forming units of oral microorganisms. This study demonstrated that tramadol accelerated stages of buccal wound healing faster than lidocaine in rabbits.

Effects of autologous platelet-rich plasma on skin healing in dogs

S. M. Hussein

Iraqi Journal of Veterinary Sciences, Volume 32, Issue 2, Pages 275-283
DOI: 10.33899/ijvs.2019.153864

This study aimed to evaluate the benefits of using platelet– rich plasma (PRP) on wound healing. The study performed on ten adult male dogs, aged 2-3 years and their weight were 18-25 kg. Under the effect of deep sedation and local subcutaneous anesthesia at the site of wound, four of (2x2) cm of full-thickness square cutaneous wounds were induced, two on each side of the lateral thoracic region of each animal. A pair of left wounds was treated by injection with 5 mL of autonomous PRP (treatment group), 2 mm lateral to the wound edges and in the wound center., immediately after wounding while, right wounds, were injected with a single dose of 5ml of sterile saline (control group) by using the same procedure as in treatment group. The clinical and histopathological evaluation of each group for five weeks, histological biopsies were taken in the days (3, 7, 14, 21, 28 and 35) of the experiment. The clinical evaluation of PRP treated wounds showed that the wound healing process (% contraction, % re-epithelization and % total wound healing) were significantly more (P<0.05) than the control wounds during the five weeks study. The histopathological results revealed that PRP treated wounds have enhanced cellularity, increased vascularity, with increased amount of granulation tissue accompanied by increase numbers of skin appendages suggesting enhanced cutaneous regeneration, than those in untreated wounds. Depending on the clinical and histopathological findings, this study confirms that local implantation of PRP leads to acceleration and improvement cutaneous wound healing.